A microfluidic paper-based sensor for iodine monitoring as a public health indicator
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Tipo de documento: | Dissertação |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.14/43907 |
Resumo: | In this work, a new microfluidic paper-based analytical device (µPAD) was developed for on-site iodide quantification. As iodine is an essential micronutrient for humans, due to its fundamental role in producing the thyroid hormones, it is a key parameter to assess health condition. Iodine deficiency (ID) has contributed to continued underdevelopment for millions of people, making it the single most important and preventable cause of brain damage. In case of not fulfilling the iodine requirements for the human body, the synthesis of thyroid hormones is impaired, causing several iodine deficiency disorders. Consequently, iodine intake needs to be monitored to prevent ID. The paper-based sensor was based on the reaction of 3,3’,5,5’-tetramethylbenzidine (TMB) with H2O2, in which iodide is a catalyst. This reaction results in the oxidation of TMB, which goes from colourless to its oxidized blue colour. The design of the µPAD consisted of two layer of filter paper discs, the top layer (Whatman 4 paper) embedded in a mixture of acetic acid and hydrogen peroxide and the bottom later (Whatman 1 paper) impregnated with TMB. The µPAD was scanned at 25 minutes and analysed in ImageJ software, where the intensity of the formed colour product was converted to absorbance values. After optimization, a limit of detection of 0.89 mg/L (7.0 µM) and a limit of quantification of 2.8 mg/L (22 µM) were attained. Different types of samples were targeted, such as urine, salt and dietary supplements, corresponding to the intake and outtake of iodide. However, the salt samples presented some interferences from chloride, and the urine samples proved to have a significantly lower iodide content than the current limits of detection of the developed method. Lastly, this method was validated by comparing the obtained results for dietary supplements with potentiometric method and no significant differences between both methods were found. The µPAD was successfully developed, as it allowed for a rapid response for analysing iodide in different samples and still being a low-cost approach, since the consumables for each device cost approximately 0.12 €. The developed device allows for an economic, simpler and faster determination of iodide, which until now required complicated and time-consuming methods, adding the advantage of not needing to pre-treatment the samples to be used. |
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A microfluidic paper-based sensor for iodine monitoring as a public health indicatorMicrofluidic paper-based analytical device (μPAD)Iodide determinationIodineIodine deficiency disordersOn-site analysisDietary supplementsDispositivo microfluídico baseado em papel (μPAD)Determinação de iodetoIodoDoenças por deficiência de iodoAnálises on siteSuplementos alimentaresDomínio/Área Científica::Ciências Médicas::Biotecnologia MédicaIn this work, a new microfluidic paper-based analytical device (µPAD) was developed for on-site iodide quantification. As iodine is an essential micronutrient for humans, due to its fundamental role in producing the thyroid hormones, it is a key parameter to assess health condition. Iodine deficiency (ID) has contributed to continued underdevelopment for millions of people, making it the single most important and preventable cause of brain damage. In case of not fulfilling the iodine requirements for the human body, the synthesis of thyroid hormones is impaired, causing several iodine deficiency disorders. Consequently, iodine intake needs to be monitored to prevent ID. The paper-based sensor was based on the reaction of 3,3’,5,5’-tetramethylbenzidine (TMB) with H2O2, in which iodide is a catalyst. This reaction results in the oxidation of TMB, which goes from colourless to its oxidized blue colour. The design of the µPAD consisted of two layer of filter paper discs, the top layer (Whatman 4 paper) embedded in a mixture of acetic acid and hydrogen peroxide and the bottom later (Whatman 1 paper) impregnated with TMB. The µPAD was scanned at 25 minutes and analysed in ImageJ software, where the intensity of the formed colour product was converted to absorbance values. After optimization, a limit of detection of 0.89 mg/L (7.0 µM) and a limit of quantification of 2.8 mg/L (22 µM) were attained. Different types of samples were targeted, such as urine, salt and dietary supplements, corresponding to the intake and outtake of iodide. However, the salt samples presented some interferences from chloride, and the urine samples proved to have a significantly lower iodide content than the current limits of detection of the developed method. Lastly, this method was validated by comparing the obtained results for dietary supplements with potentiometric method and no significant differences between both methods were found. The µPAD was successfully developed, as it allowed for a rapid response for analysing iodide in different samples and still being a low-cost approach, since the consumables for each device cost approximately 0.12 €. The developed device allows for an economic, simpler and faster determination of iodide, which until now required complicated and time-consuming methods, adding the advantage of not needing to pre-treatment the samples to be used.Neste trabalho foi desenvolvido um novo dispositivo microfluídico baseado em papel (µPAD) para a quantificação de iodeto. Como o iodo tem um papel fundamental para o funcionamento da tiroide, torna-se um micronutriente essencial para os seres humanos, revelando-se um parâmetro fundamental para avaliar o estado de saúde. A deficiência de iodo (ID) tem contribuído para o subdesenvolvimento contínuo de milhões de pessoas, tornando-se a causa mais incidente de danos cerebrais. Se os níveis de iodo necessários para o corpo humano não forem satisfeitos, diversas doenças por deficiência de iodo podem surgir. Posto isto, a ingestão de iodo deve ser monitorizada para prevenir a ID. O dispositivo em papel foi baseado na reação de 3,3’,5,5’-tetrametilbenzidina (TMB) com H2O2, na qual o iodeto é catalisador. A reação resulta na oxidação do TMB, que passa a estar na sua forma oxidada azul. O µPAD desenvolvido consistiu em duas camadas de discos de papel de filtro, em que a camada superior (Whatman 4) foi embebida numa mistura de ácido acético e peróxido de hidrogénio e a camada inferior (Whatman 1), impregnado com TMB. O dispositivo foi digitalizado aos 25 minutos e analisado no software ImageJ, onde a intensidade da cor formada foi convertida em valor de absorvância. Após otimização, um limite de deteção de 0,89 mg/L (7.0 µM) e um limite de quantificação de 2,8 mg/L (22 µM) foram obtidos. Para monitorizar a ingestão/excreção de iodeto, várias amostras foram testadas, como urina, sal e suplementos alimentares. No entanto, as amostras de sal apresentaram interferências devido ao cloreto, e as amostras de urina apresentaram níveis de iodeto abaixo do limite de deteção do método desenvolvido. Por último, este método foi validado através da comparação dos resultados obtidos para suplementos alimentares com um método e verificou-se que não havia diferenças significativas. O µPAD foi desenvolvido com sucesso, visto que permitiu uma resposta rápida para a análise de iodeto em diferentes amostras, apresentando um baixo custo, uma vez que os consumíveis de cada dispositivo custam aproximadamente 0,12€. Assim, é possível uma determinação económica, simples e rápida de iodeto, que até agora exigia métodos complicados e morosos, e sem a necessidade de pré-tratamentos de amostras.Mesquita, Raquel Beatriz Ribeiro deRangel, António Osmaro Santos SilvaVeritati - Repositório Institucional da Universidade Católica PortuguesaPereira, Mafalda Gomes2023-12-112023-112025-02-01T00:00:00Z2023-12-11T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfhttp://hdl.handle.net/10400.14/43907TID:203443128enginfo:eu-repo/semantics/embargoedAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-02-13T01:34:32Zoai:repositorio.ucp.pt:10400.14/43907Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T02:37:58.556179Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
A microfluidic paper-based sensor for iodine monitoring as a public health indicator |
title |
A microfluidic paper-based sensor for iodine monitoring as a public health indicator |
spellingShingle |
A microfluidic paper-based sensor for iodine monitoring as a public health indicator Pereira, Mafalda Gomes Microfluidic paper-based analytical device (μPAD) Iodide determination Iodine Iodine deficiency disorders On-site analysis Dietary supplements Dispositivo microfluídico baseado em papel (μPAD) Determinação de iodeto Iodo Doenças por deficiência de iodo Análises on site Suplementos alimentares Domínio/Área Científica::Ciências Médicas::Biotecnologia Médica |
title_short |
A microfluidic paper-based sensor for iodine monitoring as a public health indicator |
title_full |
A microfluidic paper-based sensor for iodine monitoring as a public health indicator |
title_fullStr |
A microfluidic paper-based sensor for iodine monitoring as a public health indicator |
title_full_unstemmed |
A microfluidic paper-based sensor for iodine monitoring as a public health indicator |
title_sort |
A microfluidic paper-based sensor for iodine monitoring as a public health indicator |
author |
Pereira, Mafalda Gomes |
author_facet |
Pereira, Mafalda Gomes |
author_role |
author |
dc.contributor.none.fl_str_mv |
Mesquita, Raquel Beatriz Ribeiro de Rangel, António Osmaro Santos Silva Veritati - Repositório Institucional da Universidade Católica Portuguesa |
dc.contributor.author.fl_str_mv |
Pereira, Mafalda Gomes |
dc.subject.por.fl_str_mv |
Microfluidic paper-based analytical device (μPAD) Iodide determination Iodine Iodine deficiency disorders On-site analysis Dietary supplements Dispositivo microfluídico baseado em papel (μPAD) Determinação de iodeto Iodo Doenças por deficiência de iodo Análises on site Suplementos alimentares Domínio/Área Científica::Ciências Médicas::Biotecnologia Médica |
topic |
Microfluidic paper-based analytical device (μPAD) Iodide determination Iodine Iodine deficiency disorders On-site analysis Dietary supplements Dispositivo microfluídico baseado em papel (μPAD) Determinação de iodeto Iodo Doenças por deficiência de iodo Análises on site Suplementos alimentares Domínio/Área Científica::Ciências Médicas::Biotecnologia Médica |
description |
In this work, a new microfluidic paper-based analytical device (µPAD) was developed for on-site iodide quantification. As iodine is an essential micronutrient for humans, due to its fundamental role in producing the thyroid hormones, it is a key parameter to assess health condition. Iodine deficiency (ID) has contributed to continued underdevelopment for millions of people, making it the single most important and preventable cause of brain damage. In case of not fulfilling the iodine requirements for the human body, the synthesis of thyroid hormones is impaired, causing several iodine deficiency disorders. Consequently, iodine intake needs to be monitored to prevent ID. The paper-based sensor was based on the reaction of 3,3’,5,5’-tetramethylbenzidine (TMB) with H2O2, in which iodide is a catalyst. This reaction results in the oxidation of TMB, which goes from colourless to its oxidized blue colour. The design of the µPAD consisted of two layer of filter paper discs, the top layer (Whatman 4 paper) embedded in a mixture of acetic acid and hydrogen peroxide and the bottom later (Whatman 1 paper) impregnated with TMB. The µPAD was scanned at 25 minutes and analysed in ImageJ software, where the intensity of the formed colour product was converted to absorbance values. After optimization, a limit of detection of 0.89 mg/L (7.0 µM) and a limit of quantification of 2.8 mg/L (22 µM) were attained. Different types of samples were targeted, such as urine, salt and dietary supplements, corresponding to the intake and outtake of iodide. However, the salt samples presented some interferences from chloride, and the urine samples proved to have a significantly lower iodide content than the current limits of detection of the developed method. Lastly, this method was validated by comparing the obtained results for dietary supplements with potentiometric method and no significant differences between both methods were found. The µPAD was successfully developed, as it allowed for a rapid response for analysing iodide in different samples and still being a low-cost approach, since the consumables for each device cost approximately 0.12 €. The developed device allows for an economic, simpler and faster determination of iodide, which until now required complicated and time-consuming methods, adding the advantage of not needing to pre-treatment the samples to be used. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-12-11 2023-11 2023-12-11T00:00:00Z 2025-02-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.14/43907 TID:203443128 |
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http://hdl.handle.net/10400.14/43907 |
identifier_str_mv |
TID:203443128 |
dc.language.iso.fl_str_mv |
eng |
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eng |
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application/pdf |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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