Are coffee silverskin extracts safe for topical use? An in vitro and in vivo approach
Autor(a) principal: | |
---|---|
Data de Publicação: | 2014 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.22/7269 |
Resumo: | Recent changes in regulatory requirements and social views on animal testing have incremented the development of reliable alternative tests for predicting skin and ocular irritation potential of products based on new raw materials. In this regard, botanical ingredients used in cosmetic products are among those materials, and should be carefully reviewed concerning the potential presence of irritant constituents. In particular, cosmetic products used on the face, in vicinity of the eyes or that may come in contact with mucous membranes, should avoid botanical ingredients that contain, or are suspected to contain, such ingredients. In this study, we aimed to evaluate the effect of a new cosmetic ingredient, namely, coffee silverskin (CS), with an in vitro skin and ocular irritation assay using reconstructed human epidermis, EpiSkin™, and human corneal epithelial model, SkinEthics™ HCE, and an in vivo assay. Three different extracts of CS were evaluated. The histology of the models after extracts applications was analysed. The in vitro results demonstrated that extracts were not classified as irritant and the histological analyses proved that extracts did not affect both models structure. The content of caffeine, 5-hydroxymethyl furfural and chlorogenic acid was quantified after the epidermal assay. The in vivo test carried out with the most promising extract (hydroalcoholic) showed that, with respect to irritant effects, these extracts can be regarded as safe for topical application. |
id |
RCAP_a31c13c275ce03d5b90dc409a052e07c |
---|---|
oai_identifier_str |
oai:recipp.ipp.pt:10400.22/7269 |
network_acronym_str |
RCAP |
network_name_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository_id_str |
7160 |
spelling |
Are coffee silverskin extracts safe for topical use? An in vitro and in vivo approachIn vitro skin culturesInterleukin-1α (IL-1α)Skin irritation potentialCoffee silverskinEpiSkinSkinEthicsRecent changes in regulatory requirements and social views on animal testing have incremented the development of reliable alternative tests for predicting skin and ocular irritation potential of products based on new raw materials. In this regard, botanical ingredients used in cosmetic products are among those materials, and should be carefully reviewed concerning the potential presence of irritant constituents. In particular, cosmetic products used on the face, in vicinity of the eyes or that may come in contact with mucous membranes, should avoid botanical ingredients that contain, or are suspected to contain, such ingredients. In this study, we aimed to evaluate the effect of a new cosmetic ingredient, namely, coffee silverskin (CS), with an in vitro skin and ocular irritation assay using reconstructed human epidermis, EpiSkin™, and human corneal epithelial model, SkinEthics™ HCE, and an in vivo assay. Three different extracts of CS were evaluated. The histology of the models after extracts applications was analysed. The in vitro results demonstrated that extracts were not classified as irritant and the histological analyses proved that extracts did not affect both models structure. The content of caffeine, 5-hydroxymethyl furfural and chlorogenic acid was quantified after the epidermal assay. The in vivo test carried out with the most promising extract (hydroalcoholic) showed that, with respect to irritant effects, these extracts can be regarded as safe for topical application.ElsevierRepositório Científico do Instituto Politécnico do PortoRodrigues, F.Pereira, C.Pimentel, F. B.Alves, R. C.Ferreira, M.Sarmento, B.Amaral, Maria HelenaBeatriz, M.Oliveira, P. P.2015-12-28T15:45:53Z2014-012014-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.22/7269eng10.1016/j.indcrop.2014.10.014info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-03-13T12:47:37Zoai:recipp.ipp.pt:10400.22/7269Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T17:27:44.425710Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Are coffee silverskin extracts safe for topical use? An in vitro and in vivo approach |
title |
Are coffee silverskin extracts safe for topical use? An in vitro and in vivo approach |
spellingShingle |
Are coffee silverskin extracts safe for topical use? An in vitro and in vivo approach Rodrigues, F. In vitro skin cultures Interleukin-1α (IL-1α) Skin irritation potential Coffee silverskin EpiSkin SkinEthics |
title_short |
Are coffee silverskin extracts safe for topical use? An in vitro and in vivo approach |
title_full |
Are coffee silverskin extracts safe for topical use? An in vitro and in vivo approach |
title_fullStr |
Are coffee silverskin extracts safe for topical use? An in vitro and in vivo approach |
title_full_unstemmed |
Are coffee silverskin extracts safe for topical use? An in vitro and in vivo approach |
title_sort |
Are coffee silverskin extracts safe for topical use? An in vitro and in vivo approach |
author |
Rodrigues, F. |
author_facet |
Rodrigues, F. Pereira, C. Pimentel, F. B. Alves, R. C. Ferreira, M. Sarmento, B. Amaral, Maria Helena Beatriz, M. Oliveira, P. P. |
author_role |
author |
author2 |
Pereira, C. Pimentel, F. B. Alves, R. C. Ferreira, M. Sarmento, B. Amaral, Maria Helena Beatriz, M. Oliveira, P. P. |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Repositório Científico do Instituto Politécnico do Porto |
dc.contributor.author.fl_str_mv |
Rodrigues, F. Pereira, C. Pimentel, F. B. Alves, R. C. Ferreira, M. Sarmento, B. Amaral, Maria Helena Beatriz, M. Oliveira, P. P. |
dc.subject.por.fl_str_mv |
In vitro skin cultures Interleukin-1α (IL-1α) Skin irritation potential Coffee silverskin EpiSkin SkinEthics |
topic |
In vitro skin cultures Interleukin-1α (IL-1α) Skin irritation potential Coffee silverskin EpiSkin SkinEthics |
description |
Recent changes in regulatory requirements and social views on animal testing have incremented the development of reliable alternative tests for predicting skin and ocular irritation potential of products based on new raw materials. In this regard, botanical ingredients used in cosmetic products are among those materials, and should be carefully reviewed concerning the potential presence of irritant constituents. In particular, cosmetic products used on the face, in vicinity of the eyes or that may come in contact with mucous membranes, should avoid botanical ingredients that contain, or are suspected to contain, such ingredients. In this study, we aimed to evaluate the effect of a new cosmetic ingredient, namely, coffee silverskin (CS), with an in vitro skin and ocular irritation assay using reconstructed human epidermis, EpiSkin™, and human corneal epithelial model, SkinEthics™ HCE, and an in vivo assay. Three different extracts of CS were evaluated. The histology of the models after extracts applications was analysed. The in vitro results demonstrated that extracts were not classified as irritant and the histological analyses proved that extracts did not affect both models structure. The content of caffeine, 5-hydroxymethyl furfural and chlorogenic acid was quantified after the epidermal assay. The in vivo test carried out with the most promising extract (hydroalcoholic) showed that, with respect to irritant effects, these extracts can be regarded as safe for topical application. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-01 2014-01-01T00:00:00Z 2015-12-28T15:45:53Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.22/7269 |
url |
http://hdl.handle.net/10400.22/7269 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1016/j.indcrop.2014.10.014 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
|
_version_ |
1799131372487442432 |