Enhancing the bioconversion of winery and olive mill waste mixtures into lignocellulolytic enzymes and animal feed by Aspergillus uvarum using a packed-bed bioreactor
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/37913 |
Resumo: | Wineries and olive oil industries are dominant agro-industrial activities in southern European regions. Olive pomace, exhausted grape marc and vine shoot trimmings are lignocellulosic residues generated by these industries, which could be valued biotechnologically. In the present work these residues were used as substrate to produce cellulases and xylanases through solid-state fermentation using Aspergillus uvarum. For that, two factorial designs (32) were first planned to optimize substrate composition, temperature and initial moisture level. Subsequently, the kinectics of cellulolytic enzymes production, fungal growth and fermented solid were characterized, Finally, the process was performed in a packed-bed bioreactor. The results showed that cellulase activity improved with the optimization processes, reaching 33.56 U/g, and with the packed-bed bioreactor aeration of 0.2 L/min, reaching 38.51 U/g. The composition of fermented solids indicated their potential use for animal feed because cellulose, hemicellulose, lignin and phenolic compounds were partially degraded 28.08, 10.78, 13.3 and 28.32% respectively, crude protein was increased from 8.47% to 17.08%, and, the mineral contents meet the requirements of main livestock. |
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Enhancing the bioconversion of winery and olive mill waste mixtures into lignocellulolytic enzymes and animal feed by Aspergillus uvarum using a packed-bed bioreactorSolid-state fermentationPacked-bed bioreactorCellulasesWinery wastesOlive mill wastesAnimal feedScience & TechnologyWineries and olive oil industries are dominant agro-industrial activities in southern European regions. Olive pomace, exhausted grape marc and vine shoot trimmings are lignocellulosic residues generated by these industries, which could be valued biotechnologically. In the present work these residues were used as substrate to produce cellulases and xylanases through solid-state fermentation using Aspergillus uvarum. For that, two factorial designs (32) were first planned to optimize substrate composition, temperature and initial moisture level. Subsequently, the kinectics of cellulolytic enzymes production, fungal growth and fermented solid were characterized, Finally, the process was performed in a packed-bed bioreactor. The results showed that cellulase activity improved with the optimization processes, reaching 33.56 U/g, and with the packed-bed bioreactor aeration of 0.2 L/min, reaching 38.51 U/g. The composition of fermented solids indicated their potential use for animal feed because cellulose, hemicellulose, lignin and phenolic compounds were partially degraded 28.08, 10.78, 13.3 and 28.32% respectively, crude protein was increased from 8.47% to 17.08%, and, the mineral contents meet the requirements of main livestock.J.M.S. was supported by Grant SFRH/BPD/84440/2012 from Fundacao para a Ciencia e Tecnologia (FCT), Portugal. L.A. was supported by Grant Incentivo/EQB/LA0023/2014 from O Novo Norte (ON.2). We thank FCT Strategic Project PEst-OE/EQB/LA0023/2013 and the Project "BioInd - Biotechnology and Bioengineering for improved Industrial and Agro-Food processes, Rer. NORTE-07-0124-FEDER-000028" co-funded by the Programa Operacional Regional do Norte (ON.2), QREN, FEDERAmerican Chemical SocietyUniversidade do MinhoSalgado, José ManuelAbrunhosa, LuísVenâncio, ArmandoDomínguez, José ManuelBelo, Isabel2015-07-132015-07-13T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/37913engSalgado, José Manuel; Abrunhosa, Luís; Dominguez, Jose M.; Venâncio, Armando; Belo, Isabel, Enhancing the bioconversion of winery and olive mill waste mixtures into lignocellulolytic enzymes and animal feed by Aspergillus uvarum using a packed-bed bioreactor. Journal of Agricultural and Food Chemistry, 63(42), 9306-9314, 20150021-85610021-856110.1021/acs.jafc.5b0213126165254info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:16:16Zoai:repositorium.sdum.uminho.pt:1822/37913Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:08:47.522476Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Enhancing the bioconversion of winery and olive mill waste mixtures into lignocellulolytic enzymes and animal feed by Aspergillus uvarum using a packed-bed bioreactor |
title |
Enhancing the bioconversion of winery and olive mill waste mixtures into lignocellulolytic enzymes and animal feed by Aspergillus uvarum using a packed-bed bioreactor |
spellingShingle |
Enhancing the bioconversion of winery and olive mill waste mixtures into lignocellulolytic enzymes and animal feed by Aspergillus uvarum using a packed-bed bioreactor Salgado, José Manuel Solid-state fermentation Packed-bed bioreactor Cellulases Winery wastes Olive mill wastes Animal feed Science & Technology |
title_short |
Enhancing the bioconversion of winery and olive mill waste mixtures into lignocellulolytic enzymes and animal feed by Aspergillus uvarum using a packed-bed bioreactor |
title_full |
Enhancing the bioconversion of winery and olive mill waste mixtures into lignocellulolytic enzymes and animal feed by Aspergillus uvarum using a packed-bed bioreactor |
title_fullStr |
Enhancing the bioconversion of winery and olive mill waste mixtures into lignocellulolytic enzymes and animal feed by Aspergillus uvarum using a packed-bed bioreactor |
title_full_unstemmed |
Enhancing the bioconversion of winery and olive mill waste mixtures into lignocellulolytic enzymes and animal feed by Aspergillus uvarum using a packed-bed bioreactor |
title_sort |
Enhancing the bioconversion of winery and olive mill waste mixtures into lignocellulolytic enzymes and animal feed by Aspergillus uvarum using a packed-bed bioreactor |
author |
Salgado, José Manuel |
author_facet |
Salgado, José Manuel Abrunhosa, Luís Venâncio, Armando Domínguez, José Manuel Belo, Isabel |
author_role |
author |
author2 |
Abrunhosa, Luís Venâncio, Armando Domínguez, José Manuel Belo, Isabel |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Salgado, José Manuel Abrunhosa, Luís Venâncio, Armando Domínguez, José Manuel Belo, Isabel |
dc.subject.por.fl_str_mv |
Solid-state fermentation Packed-bed bioreactor Cellulases Winery wastes Olive mill wastes Animal feed Science & Technology |
topic |
Solid-state fermentation Packed-bed bioreactor Cellulases Winery wastes Olive mill wastes Animal feed Science & Technology |
description |
Wineries and olive oil industries are dominant agro-industrial activities in southern European regions. Olive pomace, exhausted grape marc and vine shoot trimmings are lignocellulosic residues generated by these industries, which could be valued biotechnologically. In the present work these residues were used as substrate to produce cellulases and xylanases through solid-state fermentation using Aspergillus uvarum. For that, two factorial designs (32) were first planned to optimize substrate composition, temperature and initial moisture level. Subsequently, the kinectics of cellulolytic enzymes production, fungal growth and fermented solid were characterized, Finally, the process was performed in a packed-bed bioreactor. The results showed that cellulase activity improved with the optimization processes, reaching 33.56 U/g, and with the packed-bed bioreactor aeration of 0.2 L/min, reaching 38.51 U/g. The composition of fermented solids indicated their potential use for animal feed because cellulose, hemicellulose, lignin and phenolic compounds were partially degraded 28.08, 10.78, 13.3 and 28.32% respectively, crude protein was increased from 8.47% to 17.08%, and, the mineral contents meet the requirements of main livestock. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-07-13 2015-07-13T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/37913 |
url |
http://hdl.handle.net/1822/37913 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Salgado, José Manuel; Abrunhosa, Luís; Dominguez, Jose M.; Venâncio, Armando; Belo, Isabel, Enhancing the bioconversion of winery and olive mill waste mixtures into lignocellulolytic enzymes and animal feed by Aspergillus uvarum using a packed-bed bioreactor. Journal of Agricultural and Food Chemistry, 63(42), 9306-9314, 2015 0021-8561 0021-8561 10.1021/acs.jafc.5b02131 26165254 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
American Chemical Society |
publisher.none.fl_str_mv |
American Chemical Society |
dc.source.none.fl_str_mv |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799132511635243008 |