Bioimaging of lysosomes with a BODIPY pH-dependent fluorescent probe

Detalhes bibliográficos
Autor(a) principal: Gonçalves R. C. R.
Data de Publicação: 2022
Outros Autores: Belmonde-Reche, E., Pina, J., da Silva, M. C., Pinto, S. C. S., Gallo, J., Costa, Susana P. G., Raposo, M. Manuela M.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: https://hdl.handle.net/1822/80742
Resumo: The following supporting information can be downloaded at: https://www.mdpi.com/article/10.3390/molecules27228065/s1, Synthesis and characterization of BODIPYs 1-3 and Figure S1: Comparison between the absorption and fluorescence emission spectra of anthracene and BODIPY 1 in tetrahydrofuran solutions at room temperature; Figure S2: Normalized absorption and fluorescence emission spectra for the investigated BODIPY derivatives in acetonitrile solution at room temperature.
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spelling Bioimaging of lysosomes with a BODIPY pH-dependent fluorescent probeBODIPYsynthesisbioimaginglysosomesfluorescente probepHfluorescent probeCiências Naturais::Ciências QuímicasScience & TechnologySaúde de qualidadeThe following supporting information can be downloaded at: https://www.mdpi.com/article/10.3390/molecules27228065/s1, Synthesis and characterization of BODIPYs 1-3 and Figure S1: Comparison between the absorption and fluorescence emission spectra of anthracene and BODIPY 1 in tetrahydrofuran solutions at room temperature; Figure S2: Normalized absorption and fluorescence emission spectra for the investigated BODIPY derivatives in acetonitrile solution at room temperature.Fluorescence-based probes represent a powerful tool for noninvasive imaging of living systems in real time and with a high temporal and spatial resolution. Amongst several known fluorophores, 3-difluoroborodipyrromethene (BODIPY) derivatives have become a cornerstone for innovative fluorescent labelling applications, mainly due to their advantageous features including their facile synthesis, structural versatility and exceptional photophysical properties. In this context, we report a BODIPY-based fluorescent probe for imaging of lysosomes in living cells. The BODIPY derivative displayed a remarkable fluorescence enhancement at low pH values with a pKa* of 3.1. In vitro studies by confocal microscopy in HeLa cells demonstrated that the compound was able to permeate cell membrane and selectively label lysosome whilst remaining innocuous to the cell culture at the maximum concentration tested. Herein, the BODIPY derivative holds the promise of investigating lysosomal dynamics and function in living cells through fluorescence imaging.This research was funded by Fundação para a Ciência e Tecnologia (FCT) and FEDER (European Fund for Regional Development)-COMPETE-QRENEU through the Chemistry Research Centre of the University of Minho (ref. CQ/UM (UID/QUI/00686/2020) and the Coimbra Chemistry Centre (refs. UIDB/00313/2020 and UIDP/00313/2020), project PTDC/QUI-COL/28052/2017 and a PhD grant of R.C.R. Gonçalves (SFRH/BD/05278/2020). The NMR spectrometer Bruker Avance III 400 is part of the National NMR Network and was purchased within the framework of the National Program for Scientific Re-equipment, contract REDE/1517/RMN/2005 with funds from POCI 2010 (FEDER) and FCT.MDPIUniversidade do MinhoGonçalves R. C. R.Belmonde-Reche, E.Pina, J.da Silva, M. C.Pinto, S. C. S.Gallo, J.Costa, Susana P. G.Raposo, M. Manuela M.20222022-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfapplication/pdfhttps://hdl.handle.net/1822/80742engGonçalves, R.C.R.; Belmonte-Reche, E.; Pina, J.; Costa da Silva, M.; Pinto, S.C.S.; Gallo, J.; Costa, S.P.G.; Raposo, M.M.M. Bioimaging of Lysosomes with a BODIPY pH-Dependent Fluorescent Probe. Molecules 2022, 27, 8065. https://doi.org/10.3390/molecules2722806510.3390/molecules2722806536432168https://www.mdpi.com/1420-3049/27/22/8065info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T11:56:07ZPortal AgregadorONG
dc.title.none.fl_str_mv Bioimaging of lysosomes with a BODIPY pH-dependent fluorescent probe
title Bioimaging of lysosomes with a BODIPY pH-dependent fluorescent probe
spellingShingle Bioimaging of lysosomes with a BODIPY pH-dependent fluorescent probe
Gonçalves R. C. R.
BODIPY
synthesis
bioimaging
lysosomes
fluorescente probe
pH
fluorescent probe
Ciências Naturais::Ciências Químicas
Science & Technology
Saúde de qualidade
title_short Bioimaging of lysosomes with a BODIPY pH-dependent fluorescent probe
title_full Bioimaging of lysosomes with a BODIPY pH-dependent fluorescent probe
title_fullStr Bioimaging of lysosomes with a BODIPY pH-dependent fluorescent probe
title_full_unstemmed Bioimaging of lysosomes with a BODIPY pH-dependent fluorescent probe
title_sort Bioimaging of lysosomes with a BODIPY pH-dependent fluorescent probe
author Gonçalves R. C. R.
author_facet Gonçalves R. C. R.
Belmonde-Reche, E.
Pina, J.
da Silva, M. C.
Pinto, S. C. S.
Gallo, J.
Costa, Susana P. G.
Raposo, M. Manuela M.
author_role author
author2 Belmonde-Reche, E.
Pina, J.
da Silva, M. C.
Pinto, S. C. S.
Gallo, J.
Costa, Susana P. G.
Raposo, M. Manuela M.
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Gonçalves R. C. R.
Belmonde-Reche, E.
Pina, J.
da Silva, M. C.
Pinto, S. C. S.
Gallo, J.
Costa, Susana P. G.
Raposo, M. Manuela M.
dc.subject.por.fl_str_mv BODIPY
synthesis
bioimaging
lysosomes
fluorescente probe
pH
fluorescent probe
Ciências Naturais::Ciências Químicas
Science & Technology
Saúde de qualidade
topic BODIPY
synthesis
bioimaging
lysosomes
fluorescente probe
pH
fluorescent probe
Ciências Naturais::Ciências Químicas
Science & Technology
Saúde de qualidade
description The following supporting information can be downloaded at: https://www.mdpi.com/article/10.3390/molecules27228065/s1, Synthesis and characterization of BODIPYs 1-3 and Figure S1: Comparison between the absorption and fluorescence emission spectra of anthracene and BODIPY 1 in tetrahydrofuran solutions at room temperature; Figure S2: Normalized absorption and fluorescence emission spectra for the investigated BODIPY derivatives in acetonitrile solution at room temperature.
publishDate 2022
dc.date.none.fl_str_mv 2022
2022-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://hdl.handle.net/1822/80742
url https://hdl.handle.net/1822/80742
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Gonçalves, R.C.R.; Belmonte-Reche, E.; Pina, J.; Costa da Silva, M.; Pinto, S.C.S.; Gallo, J.; Costa, S.P.G.; Raposo, M.M.M. Bioimaging of Lysosomes with a BODIPY pH-Dependent Fluorescent Probe. Molecules 2022, 27, 8065. https://doi.org/10.3390/molecules27228065
10.3390/molecules27228065
36432168
https://www.mdpi.com/1420-3049/27/22/8065
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv
repository.mail.fl_str_mv
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