HPV16 oncoproteins induce MMPs/RECK-TIMP-2 imbalance in primary keratinocytes : possible implications in cervical carcinogenesis
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2012 |
| Outros Autores: | , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| Texto Completo: | http://hdl.handle.net/1822/24170 |
Resumo: | We thank Dr. Alda Wakamatsu, Dr. Venâncio Avancini and Dr. Alexandre Ab'Saber from Faculty of Medicine, University of São Paulo, for technical assistance and selection of tissues samples, and João Simão Pereira Sobrinho for help in protein sample processing. |
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HPV16 oncoproteins induce MMPs/RECK-TIMP-2 imbalance in primary keratinocytes : possible implications in cervical carcinogenesisScience & TechnologyWe thank Dr. Alda Wakamatsu, Dr. Venâncio Avancini and Dr. Alexandre Ab'Saber from Faculty of Medicine, University of São Paulo, for technical assistance and selection of tissues samples, and João Simão Pereira Sobrinho for help in protein sample processing.Cervical cancer is the third most common cancer in women worldwide. Persistent infection with high-risk HPV types, principally HPV16 and 18 is the main risk factor for the development of this malignancy. However, the onset of invasive tumor occurs many years after initial exposure in a minority of infected women. This suggests that other factors beyond viral infection are necessary for tumor establishment and progression. Tumor progression is characterized by an increase in secretion and activation of matrix metalloproteinases (MMPs) produced by either the tumor cells themselves or tumor-associated fibroblasts or macrophages. Increased MMPs expression, including MMP-2, MMP-9 and MT1-MMP, has been observed during cervical carcinoma progression. These proteins have been associated with degradation of ECM components, tumor invasion, metastasis and recurrence. However, few studies have evaluated the interplay between HPV infection and the expression and activity of MMPs and their regulators in cervical cancer. We analyzed the effect of HPV16 oncoproteins on the expression and activity of MMP-2, MMP-9, MT1-MMP, and their inhibitors TIMP-2 and RECK in cultures of human keratinocytes. We observed that E7 expression is associated with increased pro-MMP-9 activity in the epithelial component of organotypic cultures, while E6 and E7 oncoproteins co-expression down-regulates RECK and TIMP-2 levels in organotypic and monolayers cultures. Finally, a study conducted in human cervical tissues showed a decrease in RECK expression levels in precancer and cancer lesions. Our results indicate that HPV oncoproteins promote MMPs/RECK-TIMP-2 imbalance which may be involved in HPV-associated lesions outcome.This research was funded by FAPESP-São Paulo Research Foundation: 2005/58885-1 (to LBSC), 2008/58817-4 (to SSME) and 2008/03232-1 (to LLV); and by PRPG-USP: Pró-Reitoria de Pós-Graduação da Universidade de São Paulo and CNPq: Conselho Nacional de Pesquisa e Desenvolvimento (to SSME).Public Library of ScienceUniversidade do MinhoCardeal, Laura Beatriz da SilvaBoccardo, EnriqueTermini, LaraRabachini, TatianaAndreoli, Maria AntonietaLoreto, Celso diFilho, A. L.Villa, Luisa LinaEngler, Silvya Stuchi Maria2012-032012-03-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/24170engCardeal LBdS, Boccardo E, Termini L, Rabachini T, Andreoli MA, et al. (2012) HPV16 Oncoproteins Induce MMPs/RECK-TIMP-2 Imbalance in Primary Keratinocytes: Possible Implications in Cervical Carcinogenesis. PLoS ONE 7(3): e33585. doi:10.1371/journal.pone.00335851932-620310.1371/journal.pone.003358522438955http://dx.plos.org/10.1371/journal.pone.0033585info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:02:29Zoai:repositorium.sdum.uminho.pt:1822/24170Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:52:27.955310Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
| dc.title.none.fl_str_mv |
HPV16 oncoproteins induce MMPs/RECK-TIMP-2 imbalance in primary keratinocytes : possible implications in cervical carcinogenesis |
| title |
HPV16 oncoproteins induce MMPs/RECK-TIMP-2 imbalance in primary keratinocytes : possible implications in cervical carcinogenesis |
| spellingShingle |
HPV16 oncoproteins induce MMPs/RECK-TIMP-2 imbalance in primary keratinocytes : possible implications in cervical carcinogenesis Cardeal, Laura Beatriz da Silva Science & Technology |
| title_short |
HPV16 oncoproteins induce MMPs/RECK-TIMP-2 imbalance in primary keratinocytes : possible implications in cervical carcinogenesis |
| title_full |
HPV16 oncoproteins induce MMPs/RECK-TIMP-2 imbalance in primary keratinocytes : possible implications in cervical carcinogenesis |
| title_fullStr |
HPV16 oncoproteins induce MMPs/RECK-TIMP-2 imbalance in primary keratinocytes : possible implications in cervical carcinogenesis |
| title_full_unstemmed |
HPV16 oncoproteins induce MMPs/RECK-TIMP-2 imbalance in primary keratinocytes : possible implications in cervical carcinogenesis |
| title_sort |
HPV16 oncoproteins induce MMPs/RECK-TIMP-2 imbalance in primary keratinocytes : possible implications in cervical carcinogenesis |
| author |
Cardeal, Laura Beatriz da Silva |
| author_facet |
Cardeal, Laura Beatriz da Silva Boccardo, Enrique Termini, Lara Rabachini, Tatiana Andreoli, Maria Antonieta Loreto, Celso di Filho, A. L. Villa, Luisa Lina Engler, Silvya Stuchi Maria |
| author_role |
author |
| author2 |
Boccardo, Enrique Termini, Lara Rabachini, Tatiana Andreoli, Maria Antonieta Loreto, Celso di Filho, A. L. Villa, Luisa Lina Engler, Silvya Stuchi Maria |
| author2_role |
author author author author author author author author |
| dc.contributor.none.fl_str_mv |
Universidade do Minho |
| dc.contributor.author.fl_str_mv |
Cardeal, Laura Beatriz da Silva Boccardo, Enrique Termini, Lara Rabachini, Tatiana Andreoli, Maria Antonieta Loreto, Celso di Filho, A. L. Villa, Luisa Lina Engler, Silvya Stuchi Maria |
| dc.subject.por.fl_str_mv |
Science & Technology |
| topic |
Science & Technology |
| description |
We thank Dr. Alda Wakamatsu, Dr. Venâncio Avancini and Dr. Alexandre Ab'Saber from Faculty of Medicine, University of São Paulo, for technical assistance and selection of tissues samples, and João Simão Pereira Sobrinho for help in protein sample processing. |
| publishDate |
2012 |
| dc.date.none.fl_str_mv |
2012-03 2012-03-01T00:00:00Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
| dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/24170 |
| url |
http://hdl.handle.net/1822/24170 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
Cardeal LBdS, Boccardo E, Termini L, Rabachini T, Andreoli MA, et al. (2012) HPV16 Oncoproteins Induce MMPs/RECK-TIMP-2 Imbalance in Primary Keratinocytes: Possible Implications in Cervical Carcinogenesis. PLoS ONE 7(3): e33585. doi:10.1371/journal.pone.0033585 1932-6203 10.1371/journal.pone.0033585 22438955 http://dx.plos.org/10.1371/journal.pone.0033585 |
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info:eu-repo/semantics/openAccess |
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openAccess |
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application/pdf |
| dc.publisher.none.fl_str_mv |
Public Library of Science |
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Public Library of Science |
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reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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