Effect of cultural and nutritional conditions on the control of flocculation expression of Saccharomyces cerevisiae

Detalhes bibliográficos
Autor(a) principal: Soares, Eduardo V.
Data de Publicação: 1994
Outros Autores: Teixeira, J. A., Mota, M.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/33694
Resumo: The effect of cultural (temperature and pH) and nutritional conditions (nitrogen and carbon source) on the flocculation expression of three strains was studied. The strains' flocculation ability was determined by placing the cells in a stationary phase of growth in standard flocculation conditions. The flocculation ability of strain NCYC 1195, recently classified in the literature as the NewFlo phenotype, was more sensitive to growth temperature than Flo1 phenotype strains (NCYC 869 and NRRL Y265). The initial pH of the culture medium did not affect the flocculation ability of Flo1 phenotype strains but in the case of strain NCYC 1195 flocculation was repressed when the initial pH of the culture medium was below 3.5. Flocculation in strain NCYC 1195 was also repressed in defined culture medium; this inhibition was not related to a deficiency in any particular nitrogen source, but rather to the poor buffering capacity of the defined medium. All strains showed strong flocculation when grown in glucose, but were nonflocculent in glycerol. It was clearly demonstrated that the phenotypic expression of flocculation could be induced or repressed by changing cultural and nutritional conditions. Two distinct behaviours were also displayed with regard to the effect of the cultural conditions upon flocculation, namely the effect of pH. These different behaviours can be used to distinguish the two flocculation phenotypes.
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spelling Effect of cultural and nutritional conditions on the control of flocculation expression of Saccharomyces cerevisiaeNutrition conditionsFlocculenceSACCHAROMYCES CEREVISIAEFLOCCULATIONGROWTH TEMPERATUREPHNITROGEN SOURCESaccharomyces cereviseaeScience & TechnologyThe effect of cultural (temperature and pH) and nutritional conditions (nitrogen and carbon source) on the flocculation expression of three strains was studied. The strains' flocculation ability was determined by placing the cells in a stationary phase of growth in standard flocculation conditions. The flocculation ability of strain NCYC 1195, recently classified in the literature as the NewFlo phenotype, was more sensitive to growth temperature than Flo1 phenotype strains (NCYC 869 and NRRL Y265). The initial pH of the culture medium did not affect the flocculation ability of Flo1 phenotype strains but in the case of strain NCYC 1195 flocculation was repressed when the initial pH of the culture medium was below 3.5. Flocculation in strain NCYC 1195 was also repressed in defined culture medium; this inhibition was not related to a deficiency in any particular nitrogen source, but rather to the poor buffering capacity of the defined medium. All strains showed strong flocculation when grown in glucose, but were nonflocculent in glycerol. It was clearly demonstrated that the phenotypic expression of flocculation could be induced or repressed by changing cultural and nutritional conditions. Two distinct behaviours were also displayed with regard to the effect of the cultural conditions upon flocculation, namely the effect of pH. These different behaviours can be used to distinguish the two flocculation phenotypes.Nous avons etudie chez trois souches de Saccharomyces cerevisiae l'effet de conditions de culture (temperature et pH) et de conditions nutritives (source d'azote et de carbone) sur !'expression de la flocculation. Ce phenomene a ete etudie plairant Jes cellules en phase stationnaire dans des conditions habitue!les de flocculation. La temperature de croissance a eu plus d'influence sur le pouvoir de flocculation de la souche NCYC 1195, classee recernrnent dans la Iitterature comme phenotype NewFlo, que chez Jes souches NCYC 869 et NRRL Y265 de phenotype Flo I. Le pH initial du milieu de culture n 'avail pas d 'influence sur la flocculation des souches du phenotype Flo 1, mais dans le cas de la souche NCYC 1194 une repression etait observee lorsque le pH etait inferieur a 3,5. Cette repression de la flocculation chez la souche NCYC 1195 a aussi ete observee dans un milieu de culture defini. Cette inhibition n 'etait pas reliee a un besoin specifique d 'azote mais a un foible pouvoir tampon de ce milieu defini. Toutes Jes souches ont fortement floccule lorsque cultivees dans le glucose mais pas dans le glycerol. II est clairement demontre que !'expression phenotypique de Ja flocculation pouvait etre induite OU reprirnee par Un changernent des conditions de culture ou de nutrition. Nous avons observe deux comporternents distincts consequents a l'effet des conditions de culture sur la flocculation, particulierernent du pH. Ces comporternents differents pourraient servir a distinguer deux phenotypes de flocculation.NRC Research Press (Canadian Science Publishing)Universidade do MinhoSoares, Eduardo V.Teixeira, J. A.Mota, M.19941994-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/33694eng0008-41661480-327510.1139/m94-1358000963info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:37:11Zoai:repositorium.sdum.uminho.pt:1822/33694Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:33:26.912950Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Effect of cultural and nutritional conditions on the control of flocculation expression of Saccharomyces cerevisiae
title Effect of cultural and nutritional conditions on the control of flocculation expression of Saccharomyces cerevisiae
spellingShingle Effect of cultural and nutritional conditions on the control of flocculation expression of Saccharomyces cerevisiae
Soares, Eduardo V.
Nutrition conditions
Flocculence
SACCHAROMYCES CEREVISIAE
FLOCCULATION
GROWTH TEMPERATURE
PH
NITROGEN SOURCE
Saccharomyces cereviseae
Science & Technology
title_short Effect of cultural and nutritional conditions on the control of flocculation expression of Saccharomyces cerevisiae
title_full Effect of cultural and nutritional conditions on the control of flocculation expression of Saccharomyces cerevisiae
title_fullStr Effect of cultural and nutritional conditions on the control of flocculation expression of Saccharomyces cerevisiae
title_full_unstemmed Effect of cultural and nutritional conditions on the control of flocculation expression of Saccharomyces cerevisiae
title_sort Effect of cultural and nutritional conditions on the control of flocculation expression of Saccharomyces cerevisiae
author Soares, Eduardo V.
author_facet Soares, Eduardo V.
Teixeira, J. A.
Mota, M.
author_role author
author2 Teixeira, J. A.
Mota, M.
author2_role author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Soares, Eduardo V.
Teixeira, J. A.
Mota, M.
dc.subject.por.fl_str_mv Nutrition conditions
Flocculence
SACCHAROMYCES CEREVISIAE
FLOCCULATION
GROWTH TEMPERATURE
PH
NITROGEN SOURCE
Saccharomyces cereviseae
Science & Technology
topic Nutrition conditions
Flocculence
SACCHAROMYCES CEREVISIAE
FLOCCULATION
GROWTH TEMPERATURE
PH
NITROGEN SOURCE
Saccharomyces cereviseae
Science & Technology
description The effect of cultural (temperature and pH) and nutritional conditions (nitrogen and carbon source) on the flocculation expression of three strains was studied. The strains' flocculation ability was determined by placing the cells in a stationary phase of growth in standard flocculation conditions. The flocculation ability of strain NCYC 1195, recently classified in the literature as the NewFlo phenotype, was more sensitive to growth temperature than Flo1 phenotype strains (NCYC 869 and NRRL Y265). The initial pH of the culture medium did not affect the flocculation ability of Flo1 phenotype strains but in the case of strain NCYC 1195 flocculation was repressed when the initial pH of the culture medium was below 3.5. Flocculation in strain NCYC 1195 was also repressed in defined culture medium; this inhibition was not related to a deficiency in any particular nitrogen source, but rather to the poor buffering capacity of the defined medium. All strains showed strong flocculation when grown in glucose, but were nonflocculent in glycerol. It was clearly demonstrated that the phenotypic expression of flocculation could be induced or repressed by changing cultural and nutritional conditions. Two distinct behaviours were also displayed with regard to the effect of the cultural conditions upon flocculation, namely the effect of pH. These different behaviours can be used to distinguish the two flocculation phenotypes.
publishDate 1994
dc.date.none.fl_str_mv 1994
1994-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/33694
url http://hdl.handle.net/1822/33694
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 0008-4166
1480-3275
10.1139/m94-135
8000963
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv NRC Research Press (Canadian Science Publishing)
publisher.none.fl_str_mv NRC Research Press (Canadian Science Publishing)
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
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