Amperometric measurements of nitric oxide in erythrocytes
Autor(a) principal: | |
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Data de Publicação: | 2004 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10451/17629 |
Resumo: | © 2004 Elsevier B.V. All rights reserved. |
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7160 |
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Amperometric measurements of nitric oxide in erythrocytesNitric oxideNitric oxide sensorAmperometric methodErythrocytes© 2004 Elsevier B.V. All rights reserved.In the recent years, there has been an increase in the development of new biosensors that could be helpful in the study of various physiological processes. In this study, we report the development of a new in vitro experimental design for real-time nitric oxide (NO) amperometric measurements in erythrocyte suspensions. To achieve this, we employed human erythrocyte suspensions in sodium chloride 0.9%, pH 7 (haematocrit 0.05%). The production of NO by erythrocytes was measured with a commercial NO sensor during stimulation by l-arginine, acetylcholine, choline, atropine and velnacrine maleate (10 μM of final concentrations). We also measured the nitrite and nitrate concentrations produced by erythrocyte suspensions stimulated with the above effectors by means of the Griess reaction method. We observed that there was a direct relation between the electric current produced by the NO sensor, and the NO standard concentrations, thereby leading to a good calibration curve. The in vitro erythrocytes produced significant amperometric NO values in response to a wide range of effectors and these results have the same variation profile of the nitrites and nitrates results achieved with the Griess method. In conclusion, the amperometric NO sensor constitutes a reliable method for direct, and real-time measurement in vitro of the NO production of erythrocyte suspensions, As such, it offers a potential diagnostic technique for the evaluation of diseases, and the therapeutic progression of diseases, related to intracellular NO metabolism.This work was supported by the Faculdade de Medicina da Universidade de Lisboa and by the Fundação para a Ciência e Tecnologia, Ministério da Ciência e Tecnologia, Lisboa, Portugal.ElsevierRepositório da Universidade de LisboaCarvalho, Filomena A.Martins-Silva, JoãoCarlota, Saldanha2015-03-05T14:30:59Z20042004-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10451/17629engBiosensors and Bioelectronics 20 (2004) 505–5080956-5663http://dx.doi.org/10.1016/j.bios.2004.02.015metadata only accessinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-08T16:03:22Zoai:repositorio.ul.pt:10451/17629Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T21:37:27.140741Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Amperometric measurements of nitric oxide in erythrocytes |
title |
Amperometric measurements of nitric oxide in erythrocytes |
spellingShingle |
Amperometric measurements of nitric oxide in erythrocytes Carvalho, Filomena A. Nitric oxide Nitric oxide sensor Amperometric method Erythrocytes |
title_short |
Amperometric measurements of nitric oxide in erythrocytes |
title_full |
Amperometric measurements of nitric oxide in erythrocytes |
title_fullStr |
Amperometric measurements of nitric oxide in erythrocytes |
title_full_unstemmed |
Amperometric measurements of nitric oxide in erythrocytes |
title_sort |
Amperometric measurements of nitric oxide in erythrocytes |
author |
Carvalho, Filomena A. |
author_facet |
Carvalho, Filomena A. Martins-Silva, João Carlota, Saldanha |
author_role |
author |
author2 |
Martins-Silva, João Carlota, Saldanha |
author2_role |
author author |
dc.contributor.none.fl_str_mv |
Repositório da Universidade de Lisboa |
dc.contributor.author.fl_str_mv |
Carvalho, Filomena A. Martins-Silva, João Carlota, Saldanha |
dc.subject.por.fl_str_mv |
Nitric oxide Nitric oxide sensor Amperometric method Erythrocytes |
topic |
Nitric oxide Nitric oxide sensor Amperometric method Erythrocytes |
description |
© 2004 Elsevier B.V. All rights reserved. |
publishDate |
2004 |
dc.date.none.fl_str_mv |
2004 2004-01-01T00:00:00Z 2015-03-05T14:30:59Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10451/17629 |
url |
http://hdl.handle.net/10451/17629 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Biosensors and Bioelectronics 20 (2004) 505–508 0956-5663 http://dx.doi.org/10.1016/j.bios.2004.02.015 |
dc.rights.driver.fl_str_mv |
metadata only access info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
metadata only access |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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|
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1799134272200638464 |