Phenolic composition and bioactivity of Lavandula pedunculata (Mill.) Cav. samples fromdifferent geographical origin
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10198/19096 |
Resumo: | The aim of this study was to characterize the phenolic composition and evaluate the bioactivity of several samples of Lavandula pedunculata (Mill.) Cav, and to compare aqueous and hydroethanolic extracts. Plant materials were obtained by growing some accessions (seed samples) of various wild populations from different regions of Portugal conserved at the Portuguese Genebank in Braga. Phenolic compounds were analised by HPLC-DAD-ESI/MSn, antioxidant potential through in vitro assays (DPPH radical scavenging activity, reducing power and inhibition of lipid peroxidation), cytotoxicity on tumor cells (MCF-7, NCI-H460, HeLa and HepG2) and non-tumor (PLP2) cells, anti-inflammatory activity in rat RAW 264.7 macrophages, by the ability to inhibit NO production and antimicrobial potential by the microdilution method with INT dye (iodonitrotetrazolium chloride). Thirteen compounds were identified, being salvianolic acid B, rosmarinic acid and luteolin-7-O-glucuronide, the main compounds present, with values ranging between 44.3⁻582, 50.9⁻550, and 24.36⁻101.5 mg/g extract, respectively. L. pedunculata aqueous extract revealed a higher antioxidant potential (EC50 values between 14 to 530 μg/mL), which could be related to its higher concentration in phenolic compounds; however, the hydroethanolic extract showed a higher anti-inflammatory (lower EC50 values than 124 μg/mL) potential and antiproliferative capacity (lower GI50 values than 34 μg/mL). Thus, this study highlights the bioactive effects of this species and opens up possibilities of uses in food and pharmaceutical formulations. However, there are potential differences in such properties according to geographical origin of plant material, as in general, the samples from Alentejo presented higher results in all the bioactivities, compared with Trás-os-Montes samples. |
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Phenolic composition and bioactivity of Lavandula pedunculata (Mill.) Cav. samples fromdifferent geographical originAnti-Inflammatory AgentsAntioxidantsLavandula pedunculata (Mill.) Cav.Lipid PeroxidationPhenolsPlant ExtractsThe aim of this study was to characterize the phenolic composition and evaluate the bioactivity of several samples of Lavandula pedunculata (Mill.) Cav, and to compare aqueous and hydroethanolic extracts. Plant materials were obtained by growing some accessions (seed samples) of various wild populations from different regions of Portugal conserved at the Portuguese Genebank in Braga. Phenolic compounds were analised by HPLC-DAD-ESI/MSn, antioxidant potential through in vitro assays (DPPH radical scavenging activity, reducing power and inhibition of lipid peroxidation), cytotoxicity on tumor cells (MCF-7, NCI-H460, HeLa and HepG2) and non-tumor (PLP2) cells, anti-inflammatory activity in rat RAW 264.7 macrophages, by the ability to inhibit NO production and antimicrobial potential by the microdilution method with INT dye (iodonitrotetrazolium chloride). Thirteen compounds were identified, being salvianolic acid B, rosmarinic acid and luteolin-7-O-glucuronide, the main compounds present, with values ranging between 44.3⁻582, 50.9⁻550, and 24.36⁻101.5 mg/g extract, respectively. L. pedunculata aqueous extract revealed a higher antioxidant potential (EC50 values between 14 to 530 μg/mL), which could be related to its higher concentration in phenolic compounds; however, the hydroethanolic extract showed a higher anti-inflammatory (lower EC50 values than 124 μg/mL) potential and antiproliferative capacity (lower GI50 values than 34 μg/mL). Thus, this study highlights the bioactive effects of this species and opens up possibilities of uses in food and pharmaceutical formulations. However, there are potential differences in such properties according to geographical origin of plant material, as in general, the samples from Alentejo presented higher results in all the bioactivities, compared with Trás-os-Montes samples.The authors are grateful to the Foundation for Science and Technology (FCT, Portugal) and FEDER under Program PT2020 for financial support to CIMO (UID/AGR/00690/2013), L. Barros and R. Calhelha contracts. The authors are grateful to FEDER-Interreg España-Portugal programme for financial support through the project 0377_Iberphenol_6_E.Biblioteca Digital do IPBLopes, Catarina Leonor Afonso do ValePereira, ElianaSoković, MarinaCarvalho, Ana MariaBarata, Ana MariaLopes, Violeta R.Rocha, FilomenaCalhelha, Ricardo C.Barros, LillianFerreira, Isabel C.F.R.2019-03-07T09:48:13Z20182018-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10198/19096engLopes, Catarina; Pereira, Eliana; Soković, Marina; Carvalho, Ana Maria; Barata, Ana Maria; Lopes, Violeta; Rocha, Filomena; Calhelha, Ricardo C.; Barros, Lillian; Ferreira, Isabel C.F.R. (2018). Phenolic composition and bioactivity of Lavandula pedunculata (Mill.) Cav. samples fromdifferent geographical origin. Molecules. ISSN 1420-3049. 1037, p. 1-191420-304910.3390/molecules23051037info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-21T10:43:36Zoai:bibliotecadigital.ipb.pt:10198/19096Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T23:09:26.193974Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Phenolic composition and bioactivity of Lavandula pedunculata (Mill.) Cav. samples fromdifferent geographical origin |
title |
Phenolic composition and bioactivity of Lavandula pedunculata (Mill.) Cav. samples fromdifferent geographical origin |
spellingShingle |
Phenolic composition and bioactivity of Lavandula pedunculata (Mill.) Cav. samples fromdifferent geographical origin Lopes, Catarina Leonor Afonso do Vale Anti-Inflammatory Agents Antioxidants Lavandula pedunculata (Mill.) Cav. Lipid Peroxidation Phenols Plant Extracts |
title_short |
Phenolic composition and bioactivity of Lavandula pedunculata (Mill.) Cav. samples fromdifferent geographical origin |
title_full |
Phenolic composition and bioactivity of Lavandula pedunculata (Mill.) Cav. samples fromdifferent geographical origin |
title_fullStr |
Phenolic composition and bioactivity of Lavandula pedunculata (Mill.) Cav. samples fromdifferent geographical origin |
title_full_unstemmed |
Phenolic composition and bioactivity of Lavandula pedunculata (Mill.) Cav. samples fromdifferent geographical origin |
title_sort |
Phenolic composition and bioactivity of Lavandula pedunculata (Mill.) Cav. samples fromdifferent geographical origin |
author |
Lopes, Catarina Leonor Afonso do Vale |
author_facet |
Lopes, Catarina Leonor Afonso do Vale Pereira, Eliana Soković, Marina Carvalho, Ana Maria Barata, Ana Maria Lopes, Violeta R. Rocha, Filomena Calhelha, Ricardo C. Barros, Lillian Ferreira, Isabel C.F.R. |
author_role |
author |
author2 |
Pereira, Eliana Soković, Marina Carvalho, Ana Maria Barata, Ana Maria Lopes, Violeta R. Rocha, Filomena Calhelha, Ricardo C. Barros, Lillian Ferreira, Isabel C.F.R. |
author2_role |
author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Biblioteca Digital do IPB |
dc.contributor.author.fl_str_mv |
Lopes, Catarina Leonor Afonso do Vale Pereira, Eliana Soković, Marina Carvalho, Ana Maria Barata, Ana Maria Lopes, Violeta R. Rocha, Filomena Calhelha, Ricardo C. Barros, Lillian Ferreira, Isabel C.F.R. |
dc.subject.por.fl_str_mv |
Anti-Inflammatory Agents Antioxidants Lavandula pedunculata (Mill.) Cav. Lipid Peroxidation Phenols Plant Extracts |
topic |
Anti-Inflammatory Agents Antioxidants Lavandula pedunculata (Mill.) Cav. Lipid Peroxidation Phenols Plant Extracts |
description |
The aim of this study was to characterize the phenolic composition and evaluate the bioactivity of several samples of Lavandula pedunculata (Mill.) Cav, and to compare aqueous and hydroethanolic extracts. Plant materials were obtained by growing some accessions (seed samples) of various wild populations from different regions of Portugal conserved at the Portuguese Genebank in Braga. Phenolic compounds were analised by HPLC-DAD-ESI/MSn, antioxidant potential through in vitro assays (DPPH radical scavenging activity, reducing power and inhibition of lipid peroxidation), cytotoxicity on tumor cells (MCF-7, NCI-H460, HeLa and HepG2) and non-tumor (PLP2) cells, anti-inflammatory activity in rat RAW 264.7 macrophages, by the ability to inhibit NO production and antimicrobial potential by the microdilution method with INT dye (iodonitrotetrazolium chloride). Thirteen compounds were identified, being salvianolic acid B, rosmarinic acid and luteolin-7-O-glucuronide, the main compounds present, with values ranging between 44.3⁻582, 50.9⁻550, and 24.36⁻101.5 mg/g extract, respectively. L. pedunculata aqueous extract revealed a higher antioxidant potential (EC50 values between 14 to 530 μg/mL), which could be related to its higher concentration in phenolic compounds; however, the hydroethanolic extract showed a higher anti-inflammatory (lower EC50 values than 124 μg/mL) potential and antiproliferative capacity (lower GI50 values than 34 μg/mL). Thus, this study highlights the bioactive effects of this species and opens up possibilities of uses in food and pharmaceutical formulations. However, there are potential differences in such properties according to geographical origin of plant material, as in general, the samples from Alentejo presented higher results in all the bioactivities, compared with Trás-os-Montes samples. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018 2018-01-01T00:00:00Z 2019-03-07T09:48:13Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10198/19096 |
url |
http://hdl.handle.net/10198/19096 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Lopes, Catarina; Pereira, Eliana; Soković, Marina; Carvalho, Ana Maria; Barata, Ana Maria; Lopes, Violeta; Rocha, Filomena; Calhelha, Ricardo C.; Barros, Lillian; Ferreira, Isabel C.F.R. (2018). Phenolic composition and bioactivity of Lavandula pedunculata (Mill.) Cav. samples fromdifferent geographical origin. Molecules. ISSN 1420-3049. 1037, p. 1-19 1420-3049 10.3390/molecules23051037 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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