Bioactive albumin functionalized polylactic acid membranes for improved biocompatibility
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/11110/615 |
Resumo: | Biocompatibility is a major challenge for successful application of many biomaterials. In this study the ability to coat chemically and enzymatically activated poly(L-lactic acid) (PLA) membranes with heat denatured human serum albumin to improve biocompatibility was investigated. PLA membranes hydrolyzed with NaOH or cutinase and then treated with 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide, hydrochloride (EDAC) as a heterobifunctional cross-linker promoted the coupling single bondCOOH groups on PLA membranes and single bondNH2 groups of heat denatured human serum albumin. This resulted in increased hydrophilicity (lowest water contact angles of 43° and 35°) and highest antioxidant activity (quenching of 79 μM and 115 μM tetramethylazobisquinone (TMAMQ) for NaOH and cutinase pretreated membranes, respectively). FTIR analysis of modified PLA membranes showed new peaks attributed to human serum albumin (amide bond, NH2 and side chain stretching) appearing within 3600–3000 cm−1 and 1700–1500 cm−1 (Fig. 3). MTT studies also showed that osteoblasts-like and MC-3T3-E1 cells viability increased 2.4 times as compared to untreated PLA membranes. The study therefore shows that this strategy of modifying the surfaces of PLA polymers could significantly improve biocompatibility. |
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Bioactive albumin functionalized polylactic acid membranes for improved biocompatibilityBioactiveHuman SerumPolylactic acid MembranesFunctionalizationCell viabilityCytocompatibilityBiocompatibility is a major challenge for successful application of many biomaterials. In this study the ability to coat chemically and enzymatically activated poly(L-lactic acid) (PLA) membranes with heat denatured human serum albumin to improve biocompatibility was investigated. PLA membranes hydrolyzed with NaOH or cutinase and then treated with 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide, hydrochloride (EDAC) as a heterobifunctional cross-linker promoted the coupling single bondCOOH groups on PLA membranes and single bondNH2 groups of heat denatured human serum albumin. This resulted in increased hydrophilicity (lowest water contact angles of 43° and 35°) and highest antioxidant activity (quenching of 79 μM and 115 μM tetramethylazobisquinone (TMAMQ) for NaOH and cutinase pretreated membranes, respectively). FTIR analysis of modified PLA membranes showed new peaks attributed to human serum albumin (amide bond, NH2 and side chain stretching) appearing within 3600–3000 cm−1 and 1700–1500 cm−1 (Fig. 3). MTT studies also showed that osteoblasts-like and MC-3T3-E1 cells viability increased 2.4 times as compared to untreated PLA membranes. The study therefore shows that this strategy of modifying the surfaces of PLA polymers could significantly improve biocompatibility.This work has been supported by the Federal Ministry of Economy, Family and Youth (BMWFJ), the Federal Ministry of Traffic, Innovation and Technology (bmvit), the Styrian Business Promotion Agency SFG, the Standortagentur Tirol and ZIT – Technology Agency of the City of Vienna through the COMET-Funding Program managed by the Austrian Research Promotion Agency FFG and EU-NOVO Project.2014-02-19T16:37:38Z2014-02-19T16:37:38Z2013-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://hdl.handle.net/11110/615oai:ciencipca.ipca.pt:11110/615eng1381-5148http://hdl.handle.net/11110/615Nyanhongo, G. S.Díaz Rodríguez, R.Nugroho Prasetyo, E.Caparros, C.Ribeiro, C.Sencadas, V.Lanceros.Mendez, S.Herrero Acero, E.Guebitza, G. M:info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2022-09-05T12:52:11Zoai:ciencipca.ipca.pt:11110/615Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T15:01:04.741620Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Bioactive albumin functionalized polylactic acid membranes for improved biocompatibility |
title |
Bioactive albumin functionalized polylactic acid membranes for improved biocompatibility |
spellingShingle |
Bioactive albumin functionalized polylactic acid membranes for improved biocompatibility Nyanhongo, G. S. Bioactive Human Serum Polylactic acid Membranes Functionalization Cell viability Cytocompatibility |
title_short |
Bioactive albumin functionalized polylactic acid membranes for improved biocompatibility |
title_full |
Bioactive albumin functionalized polylactic acid membranes for improved biocompatibility |
title_fullStr |
Bioactive albumin functionalized polylactic acid membranes for improved biocompatibility |
title_full_unstemmed |
Bioactive albumin functionalized polylactic acid membranes for improved biocompatibility |
title_sort |
Bioactive albumin functionalized polylactic acid membranes for improved biocompatibility |
author |
Nyanhongo, G. S. |
author_facet |
Nyanhongo, G. S. Díaz Rodríguez, R. Nugroho Prasetyo, E. Caparros, C. Ribeiro, C. Sencadas, V. Lanceros.Mendez, S. Herrero Acero, E. Guebitza, G. M: |
author_role |
author |
author2 |
Díaz Rodríguez, R. Nugroho Prasetyo, E. Caparros, C. Ribeiro, C. Sencadas, V. Lanceros.Mendez, S. Herrero Acero, E. Guebitza, G. M: |
author2_role |
author author author author author author author author |
dc.contributor.author.fl_str_mv |
Nyanhongo, G. S. Díaz Rodríguez, R. Nugroho Prasetyo, E. Caparros, C. Ribeiro, C. Sencadas, V. Lanceros.Mendez, S. Herrero Acero, E. Guebitza, G. M: |
dc.subject.por.fl_str_mv |
Bioactive Human Serum Polylactic acid Membranes Functionalization Cell viability Cytocompatibility |
topic |
Bioactive Human Serum Polylactic acid Membranes Functionalization Cell viability Cytocompatibility |
description |
Biocompatibility is a major challenge for successful application of many biomaterials. In this study the ability to coat chemically and enzymatically activated poly(L-lactic acid) (PLA) membranes with heat denatured human serum albumin to improve biocompatibility was investigated. PLA membranes hydrolyzed with NaOH or cutinase and then treated with 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide, hydrochloride (EDAC) as a heterobifunctional cross-linker promoted the coupling single bondCOOH groups on PLA membranes and single bondNH2 groups of heat denatured human serum albumin. This resulted in increased hydrophilicity (lowest water contact angles of 43° and 35°) and highest antioxidant activity (quenching of 79 μM and 115 μM tetramethylazobisquinone (TMAMQ) for NaOH and cutinase pretreated membranes, respectively). FTIR analysis of modified PLA membranes showed new peaks attributed to human serum albumin (amide bond, NH2 and side chain stretching) appearing within 3600–3000 cm−1 and 1700–1500 cm−1 (Fig. 3). MTT studies also showed that osteoblasts-like and MC-3T3-E1 cells viability increased 2.4 times as compared to untreated PLA membranes. The study therefore shows that this strategy of modifying the surfaces of PLA polymers could significantly improve biocompatibility. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-01-01T00:00:00Z 2014-02-19T16:37:38Z 2014-02-19T16:37:38Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/11110/615 oai:ciencipca.ipca.pt:11110/615 |
url |
http://hdl.handle.net/11110/615 |
identifier_str_mv |
oai:ciencipca.ipca.pt:11110/615 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
1381-5148 http://hdl.handle.net/11110/615 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799129880810487808 |