Low energy nanoemulsions as a carrier of thyme and lemon balm essential oils

Detalhes bibliográficos
Autor(a) principal: Sampaio, Cátia I.
Data de Publicação: 2022
Outros Autores: Bourbon, Ana Isabel Juncá Sottomayor Lisboa, Gonçalves, Catarina, Pastrana, Lorenzo M., Dias, Alice, Cerqueira, Miguel Ângelo Parente Ribeiro
Tipo de documento: Artigo
Idioma: por
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: https://hdl.handle.net/1822/89419
Resumo: The aim of this work was to produce monomodal and stable thyme and lemon balm-loaded nanoemulsions (NE), through a low energy approach. NEs were prepared by the emulsion phase inversion method using sunflower oil as carrier and Tween 80 as surfactant. Different surfactant-to-oil ratios (SOR) and essential oil (EO) loadings were evaluated. Nanoemulsions presented a monomodal diameter distribution below 200 nm and high negative zeta potential (>-40 mV). Nanoemulsions loaded with EOs were physically stable for 3 months at 4 degrees C and 20 degrees C. Nanoemulsions with thyme EO presented the highest radical scavenging activity by DPPH and ABTS tests. Loaded nanoemulsions were not effective against Escherichia coli but could inhibit Staphylococcus aureus. Cytotoxicity studies using cell counting kit-8 and resazurin assays revealed a dose-responsive effect of nanoemulsions on Caco-2 cells after incubation for 24 h. A concentration of 100 mu g oil/mL of NE for unloaded and thyme loaded and 50 mu g oil/mL of NE for lemon balm loaded showed to be safe to use.
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spelling Low energy nanoemulsions as a carrier of thyme and lemon balm essential oilsLemon balm oilLow energy emulsificationThyme oilNanoencapsulationCiências Naturais::Ciências QuímicasScience & TechnologyProteger a vida terrestreThe aim of this work was to produce monomodal and stable thyme and lemon balm-loaded nanoemulsions (NE), through a low energy approach. NEs were prepared by the emulsion phase inversion method using sunflower oil as carrier and Tween 80 as surfactant. Different surfactant-to-oil ratios (SOR) and essential oil (EO) loadings were evaluated. Nanoemulsions presented a monomodal diameter distribution below 200 nm and high negative zeta potential (>-40 mV). Nanoemulsions loaded with EOs were physically stable for 3 months at 4 degrees C and 20 degrees C. Nanoemulsions with thyme EO presented the highest radical scavenging activity by DPPH and ABTS tests. Loaded nanoemulsions were not effective against Escherichia coli but could inhibit Staphylococcus aureus. Cytotoxicity studies using cell counting kit-8 and resazurin assays revealed a dose-responsive effect of nanoemulsions on Caco-2 cells after incubation for 24 h. A concentration of 100 mu g oil/mL of NE for unloaded and thyme loaded and 50 mu g oil/mL of NE for lemon balm loaded showed to be safe to use.This research received funding from the H2020 research and inno-vation programme under the Marie Sklodowska-Curie grant agreement No 778388 (H2020 MSCA-RISE-2017, FODIAC) , and MICRODIGEST project (grant agreement 037716) co-funded by FCT and ERDF through COMPETE2020. We would like to thank the Earth Essences for its kind supply of essential oils.ElsevierUniversidade do MinhoSampaio, Cátia I.Bourbon, Ana Isabel Juncá Sottomayor LisboaGonçalves, CatarinaPastrana, Lorenzo M.Dias, AliceCerqueira, Miguel Ângelo Parente Ribeiro2022-01-152022-01-15T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/1822/89419porSampaio, C. I., Bourbon, A. I., Gonçalves, C., Pastrana, L. M., Dias, A. M., & Cerqueira, M. A. (2022, January). Low energy nanoemulsions as carriers of thyme and lemon balm essential oils. Lwt. Elsevier BV. http://doi.org/10.1016/j.lwt.2021.1127480023-643810.1016/j.lwt.2021.112748https://www.sciencedirect.com/science/article/pii/S0023643821019010info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-16T01:20:31Zoai:repositorium.sdum.uminho.pt:1822/89419Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T04:01:04.753009Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Low energy nanoemulsions as a carrier of thyme and lemon balm essential oils
title Low energy nanoemulsions as a carrier of thyme and lemon balm essential oils
spellingShingle Low energy nanoemulsions as a carrier of thyme and lemon balm essential oils
Sampaio, Cátia I.
Lemon balm oil
Low energy emulsification
Thyme oil
Nanoencapsulation
Ciências Naturais::Ciências Químicas
Science & Technology
Proteger a vida terrestre
title_short Low energy nanoemulsions as a carrier of thyme and lemon balm essential oils
title_full Low energy nanoemulsions as a carrier of thyme and lemon balm essential oils
title_fullStr Low energy nanoemulsions as a carrier of thyme and lemon balm essential oils
title_full_unstemmed Low energy nanoemulsions as a carrier of thyme and lemon balm essential oils
title_sort Low energy nanoemulsions as a carrier of thyme and lemon balm essential oils
author Sampaio, Cátia I.
author_facet Sampaio, Cátia I.
Bourbon, Ana Isabel Juncá Sottomayor Lisboa
Gonçalves, Catarina
Pastrana, Lorenzo M.
Dias, Alice
Cerqueira, Miguel Ângelo Parente Ribeiro
author_role author
author2 Bourbon, Ana Isabel Juncá Sottomayor Lisboa
Gonçalves, Catarina
Pastrana, Lorenzo M.
Dias, Alice
Cerqueira, Miguel Ângelo Parente Ribeiro
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Sampaio, Cátia I.
Bourbon, Ana Isabel Juncá Sottomayor Lisboa
Gonçalves, Catarina
Pastrana, Lorenzo M.
Dias, Alice
Cerqueira, Miguel Ângelo Parente Ribeiro
dc.subject.por.fl_str_mv Lemon balm oil
Low energy emulsification
Thyme oil
Nanoencapsulation
Ciências Naturais::Ciências Químicas
Science & Technology
Proteger a vida terrestre
topic Lemon balm oil
Low energy emulsification
Thyme oil
Nanoencapsulation
Ciências Naturais::Ciências Químicas
Science & Technology
Proteger a vida terrestre
description The aim of this work was to produce monomodal and stable thyme and lemon balm-loaded nanoemulsions (NE), through a low energy approach. NEs were prepared by the emulsion phase inversion method using sunflower oil as carrier and Tween 80 as surfactant. Different surfactant-to-oil ratios (SOR) and essential oil (EO) loadings were evaluated. Nanoemulsions presented a monomodal diameter distribution below 200 nm and high negative zeta potential (>-40 mV). Nanoemulsions loaded with EOs were physically stable for 3 months at 4 degrees C and 20 degrees C. Nanoemulsions with thyme EO presented the highest radical scavenging activity by DPPH and ABTS tests. Loaded nanoemulsions were not effective against Escherichia coli but could inhibit Staphylococcus aureus. Cytotoxicity studies using cell counting kit-8 and resazurin assays revealed a dose-responsive effect of nanoemulsions on Caco-2 cells after incubation for 24 h. A concentration of 100 mu g oil/mL of NE for unloaded and thyme loaded and 50 mu g oil/mL of NE for lemon balm loaded showed to be safe to use.
publishDate 2022
dc.date.none.fl_str_mv 2022-01-15
2022-01-15T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://hdl.handle.net/1822/89419
url https://hdl.handle.net/1822/89419
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv Sampaio, C. I., Bourbon, A. I., Gonçalves, C., Pastrana, L. M., Dias, A. M., & Cerqueira, M. A. (2022, January). Low energy nanoemulsions as carriers of thyme and lemon balm essential oils. Lwt. Elsevier BV. http://doi.org/10.1016/j.lwt.2021.112748
0023-6438
10.1016/j.lwt.2021.112748
https://www.sciencedirect.com/science/article/pii/S0023643821019010
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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