Low energy nanoemulsions as a carrier of thyme and lemon balm essential oils
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | https://hdl.handle.net/1822/89419 |
Resumo: | The aim of this work was to produce monomodal and stable thyme and lemon balm-loaded nanoemulsions (NE), through a low energy approach. NEs were prepared by the emulsion phase inversion method using sunflower oil as carrier and Tween 80 as surfactant. Different surfactant-to-oil ratios (SOR) and essential oil (EO) loadings were evaluated. Nanoemulsions presented a monomodal diameter distribution below 200 nm and high negative zeta potential (>-40 mV). Nanoemulsions loaded with EOs were physically stable for 3 months at 4 degrees C and 20 degrees C. Nanoemulsions with thyme EO presented the highest radical scavenging activity by DPPH and ABTS tests. Loaded nanoemulsions were not effective against Escherichia coli but could inhibit Staphylococcus aureus. Cytotoxicity studies using cell counting kit-8 and resazurin assays revealed a dose-responsive effect of nanoemulsions on Caco-2 cells after incubation for 24 h. A concentration of 100 mu g oil/mL of NE for unloaded and thyme loaded and 50 mu g oil/mL of NE for lemon balm loaded showed to be safe to use. |
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Low energy nanoemulsions as a carrier of thyme and lemon balm essential oilsLemon balm oilLow energy emulsificationThyme oilNanoencapsulationCiências Naturais::Ciências QuímicasScience & TechnologyProteger a vida terrestreThe aim of this work was to produce monomodal and stable thyme and lemon balm-loaded nanoemulsions (NE), through a low energy approach. NEs were prepared by the emulsion phase inversion method using sunflower oil as carrier and Tween 80 as surfactant. Different surfactant-to-oil ratios (SOR) and essential oil (EO) loadings were evaluated. Nanoemulsions presented a monomodal diameter distribution below 200 nm and high negative zeta potential (>-40 mV). Nanoemulsions loaded with EOs were physically stable for 3 months at 4 degrees C and 20 degrees C. Nanoemulsions with thyme EO presented the highest radical scavenging activity by DPPH and ABTS tests. Loaded nanoemulsions were not effective against Escherichia coli but could inhibit Staphylococcus aureus. Cytotoxicity studies using cell counting kit-8 and resazurin assays revealed a dose-responsive effect of nanoemulsions on Caco-2 cells after incubation for 24 h. A concentration of 100 mu g oil/mL of NE for unloaded and thyme loaded and 50 mu g oil/mL of NE for lemon balm loaded showed to be safe to use.This research received funding from the H2020 research and inno-vation programme under the Marie Sklodowska-Curie grant agreement No 778388 (H2020 MSCA-RISE-2017, FODIAC) , and MICRODIGEST project (grant agreement 037716) co-funded by FCT and ERDF through COMPETE2020. We would like to thank the Earth Essences for its kind supply of essential oils.ElsevierUniversidade do MinhoSampaio, Cátia I.Bourbon, Ana Isabel Juncá Sottomayor LisboaGonçalves, CatarinaPastrana, Lorenzo M.Dias, AliceCerqueira, Miguel Ângelo Parente Ribeiro2022-01-152022-01-15T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/1822/89419porSampaio, C. I., Bourbon, A. I., Gonçalves, C., Pastrana, L. M., Dias, A. M., & Cerqueira, M. A. (2022, January). Low energy nanoemulsions as carriers of thyme and lemon balm essential oils. Lwt. Elsevier BV. http://doi.org/10.1016/j.lwt.2021.1127480023-643810.1016/j.lwt.2021.112748https://www.sciencedirect.com/science/article/pii/S0023643821019010info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-16T01:20:31Zoai:repositorium.sdum.uminho.pt:1822/89419Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T04:01:04.753009Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Low energy nanoemulsions as a carrier of thyme and lemon balm essential oils |
title |
Low energy nanoemulsions as a carrier of thyme and lemon balm essential oils |
spellingShingle |
Low energy nanoemulsions as a carrier of thyme and lemon balm essential oils Sampaio, Cátia I. Lemon balm oil Low energy emulsification Thyme oil Nanoencapsulation Ciências Naturais::Ciências Químicas Science & Technology Proteger a vida terrestre |
title_short |
Low energy nanoemulsions as a carrier of thyme and lemon balm essential oils |
title_full |
Low energy nanoemulsions as a carrier of thyme and lemon balm essential oils |
title_fullStr |
Low energy nanoemulsions as a carrier of thyme and lemon balm essential oils |
title_full_unstemmed |
Low energy nanoemulsions as a carrier of thyme and lemon balm essential oils |
title_sort |
Low energy nanoemulsions as a carrier of thyme and lemon balm essential oils |
author |
Sampaio, Cátia I. |
author_facet |
Sampaio, Cátia I. Bourbon, Ana Isabel Juncá Sottomayor Lisboa Gonçalves, Catarina Pastrana, Lorenzo M. Dias, Alice Cerqueira, Miguel Ângelo Parente Ribeiro |
author_role |
author |
author2 |
Bourbon, Ana Isabel Juncá Sottomayor Lisboa Gonçalves, Catarina Pastrana, Lorenzo M. Dias, Alice Cerqueira, Miguel Ângelo Parente Ribeiro |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Sampaio, Cátia I. Bourbon, Ana Isabel Juncá Sottomayor Lisboa Gonçalves, Catarina Pastrana, Lorenzo M. Dias, Alice Cerqueira, Miguel Ângelo Parente Ribeiro |
dc.subject.por.fl_str_mv |
Lemon balm oil Low energy emulsification Thyme oil Nanoencapsulation Ciências Naturais::Ciências Químicas Science & Technology Proteger a vida terrestre |
topic |
Lemon balm oil Low energy emulsification Thyme oil Nanoencapsulation Ciências Naturais::Ciências Químicas Science & Technology Proteger a vida terrestre |
description |
The aim of this work was to produce monomodal and stable thyme and lemon balm-loaded nanoemulsions (NE), through a low energy approach. NEs were prepared by the emulsion phase inversion method using sunflower oil as carrier and Tween 80 as surfactant. Different surfactant-to-oil ratios (SOR) and essential oil (EO) loadings were evaluated. Nanoemulsions presented a monomodal diameter distribution below 200 nm and high negative zeta potential (>-40 mV). Nanoemulsions loaded with EOs were physically stable for 3 months at 4 degrees C and 20 degrees C. Nanoemulsions with thyme EO presented the highest radical scavenging activity by DPPH and ABTS tests. Loaded nanoemulsions were not effective against Escherichia coli but could inhibit Staphylococcus aureus. Cytotoxicity studies using cell counting kit-8 and resazurin assays revealed a dose-responsive effect of nanoemulsions on Caco-2 cells after incubation for 24 h. A concentration of 100 mu g oil/mL of NE for unloaded and thyme loaded and 50 mu g oil/mL of NE for lemon balm loaded showed to be safe to use. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-01-15 2022-01-15T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://hdl.handle.net/1822/89419 |
url |
https://hdl.handle.net/1822/89419 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
Sampaio, C. I., Bourbon, A. I., Gonçalves, C., Pastrana, L. M., Dias, A. M., & Cerqueira, M. A. (2022, January). Low energy nanoemulsions as carriers of thyme and lemon balm essential oils. Lwt. Elsevier BV. http://doi.org/10.1016/j.lwt.2021.112748 0023-6438 10.1016/j.lwt.2021.112748 https://www.sciencedirect.com/science/article/pii/S0023643821019010 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799138184916893696 |