Accuracy of the new rapid test for monitoring adalimumab levels

Detalhes bibliográficos
Autor(a) principal: Rocha, Cátia
Data de Publicação: 2019
Outros Autores: Afonso, Joana, Lago, Paula, Arroja, Bruno, Vieira, Ana I., Dias, Cláudia C., Magro, Fernando
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10451/39728
Resumo: © The Author(s), 2019.
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spelling Accuracy of the new rapid test for monitoring adalimumab levelsAdalimumabQuantum Blue® AdalimumabTherapeutic drug monitoring© The Author(s), 2019.Background: The loss of response to adalimumab (ADL) has been related to low serum concentrations at trough. Currently, most methods commercially available for the quantification of ADL are enzyme-linked immunosorbent assay (ELISA) based, with a turnaround time of approximately 8 h, delaying the target dosage adjustment to the subsequent infusion. In this study, we aimed to evaluate the performance of the newly available rapid-test ADL quantification assay by comparing it with three established ELISA methods, using spiked samples and a set of clinical samples. Methods: Spiked samples from control donors and 120 serum samples from inflammatory bowel disease (IBD) patients undergoing ADL therapy were quantified using lateral flow Quantum Blue® Adalimumab and, the ELISA formats from Immundiagnostik, R-Biopharm and an in-house assay. Results: The rapid-test assay had intraclass correlation coefficients of 0.590, 0.864 and 0.761 when comparing with the Immundiagnostik, R-Biopharm and in-house assays, respectively. For the five therapeutic windows, the accuracy was high: ADL rapid test compared with the Immundiagnostik (58–88%); R-Biopharm, 68–89%; and in house, 60–88%; and kappa statistics revealed 0.492–0.602, 0.531–0.659 and 0.545–0.682, respectively. Conclusions: The Quantum Blue® Adalimumab assay can replace the commonly used ELISAbased ADL quantification kits and it is a reliable alternative to these methods. This rapidtest assay enables the quantitative determination of ADL serum trough level in only 15 min. The developed assay allows measurement of ADL over a wide range. Hence, it represents a valuable tool for the clinician to assess the ADL trough level.This work was supported by the Portuguese IBD Group (GEDII, Grupo de Estudo da Doença Inflamatória Intestinal).SAGE PublicationsRepositório da Universidade de LisboaRocha, CátiaAfonso, JoanaLago, PaulaArroja, BrunoVieira, Ana I.Dias, Cláudia C.Magro, Fernando2019-10-08T11:13:15Z20192019-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10451/39728engTher Adv Gastroenterol 2019, Vol. 12: 1–111756-283X10.1177/1756284819828238info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-08T16:38:35Zoai:repositorio.ul.pt:10451/39728Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T21:53:31.138393Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Accuracy of the new rapid test for monitoring adalimumab levels
title Accuracy of the new rapid test for monitoring adalimumab levels
spellingShingle Accuracy of the new rapid test for monitoring adalimumab levels
Rocha, Cátia
Adalimumab
Quantum Blue® Adalimumab
Therapeutic drug monitoring
title_short Accuracy of the new rapid test for monitoring adalimumab levels
title_full Accuracy of the new rapid test for monitoring adalimumab levels
title_fullStr Accuracy of the new rapid test for monitoring adalimumab levels
title_full_unstemmed Accuracy of the new rapid test for monitoring adalimumab levels
title_sort Accuracy of the new rapid test for monitoring adalimumab levels
author Rocha, Cátia
author_facet Rocha, Cátia
Afonso, Joana
Lago, Paula
Arroja, Bruno
Vieira, Ana I.
Dias, Cláudia C.
Magro, Fernando
author_role author
author2 Afonso, Joana
Lago, Paula
Arroja, Bruno
Vieira, Ana I.
Dias, Cláudia C.
Magro, Fernando
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Rocha, Cátia
Afonso, Joana
Lago, Paula
Arroja, Bruno
Vieira, Ana I.
Dias, Cláudia C.
Magro, Fernando
dc.subject.por.fl_str_mv Adalimumab
Quantum Blue® Adalimumab
Therapeutic drug monitoring
topic Adalimumab
Quantum Blue® Adalimumab
Therapeutic drug monitoring
description © The Author(s), 2019.
publishDate 2019
dc.date.none.fl_str_mv 2019-10-08T11:13:15Z
2019
2019-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10451/39728
url http://hdl.handle.net/10451/39728
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Ther Adv Gastroenterol 2019, Vol. 12: 1–11
1756-283X
10.1177/1756284819828238
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv SAGE Publications
publisher.none.fl_str_mv SAGE Publications
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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