Genotoxicity and Gene Expression in the Rat Lung Tissue following Instillation and Inhalation of Different Variants of Amorphous Silica Nanomaterials (aSiO2 NM)

Detalhes bibliográficos
Autor(a) principal: Brandão, Fátima
Data de Publicação: 2021
Outros Autores: Costa, Carla, Bessa, Maria João, Dumortier, Elise, Debacq-Chainiaux, Florence, Hubaux, Roland, Salmon, Michel, Laloy, Julie, Stan, Miruna S., Hermenean, Anca, Gharbia, Sami, Dinischiotu, Anca, Bannuscher, Anne, Hellack, Bryan, Haase, Andrea, Fraga, Sónia, Teixeira, João
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.18/7909
Resumo: This article belongs to the Special Issue Cytotoxicity and Genotoxicity of Nanomaterials.
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spelling Genotoxicity and Gene Expression in the Rat Lung Tissue following Instillation and Inhalation of Different Variants of Amorphous Silica Nanomaterials (aSiO2 NM)ToxicologySilica NanomaterialsIn vivo InhalationIn vivo InstillationRat LungDNA DamageGene ExpressionNanomaterialsGenotoxicidade AmbientalThis article belongs to the Special Issue Cytotoxicity and Genotoxicity of Nanomaterials.Several reports on amorphous silica nanomaterial (aSiO2 NM) toxicity have been questioning their safety. Herein, we investigated the in vivo pulmonary toxicity of four variants of aSiO2 NM: SiO2_15_Unmod, SiO2_15_Amino, SiO2_7 and SiO2_40. We focused on alterations in lung DNA and protein integrity, and gene expression following single intratracheal instillation in rats. Additionally, a short-term inhalation study (STIS) was carried out for SiO2_7, using TiO2_NM105 as a benchmark NM. In the instillation study, a significant but slight increase in oxidative DNA damage in rats exposed to the highest instilled dose (0.36 mg/rat) of SiO2_15_Amino was observed in the recovery (R) group. Exposure to SiO2_7 or SiO2_40 markedly increased oxidative DNA lesions in rat lung cells of the exposure (E) group at every tested dose. This damage seems to be repaired, since no changes compared to controls were observed in the R groups. In STIS, a significant increase in DNA strand breaks of the lung cells exposed to 0.5 mg/m3 of SiO2_7 or 50 mg/m3 of TiO2_NM105 was observed in both groups. The detected gene expression changes suggest that oxidative stress and/or inflammation pathways are likely implicated in the induction of (oxidative) DNA damage. Overall, all tested aSiO2 NM were not associated with marked in vivo toxicity following instillation or STIS. The genotoxicity findings for SiO2_7 from instillation and STIS are concordant; however, changes in STIS animals were more permanent/difficult to revert.This work was funded by the Portuguese Foundation for Science and Technology (FCT) through ERA-NET SIINN project NanoToxClass (SIINN/0001/2013). FB and MJB are recipients of FCT PhD scholarships (SFRH/BD/101060/2014 and SFRH/BD/12046/2016). Thanks are due to FCT/MCTES for the financial support through national funds to EPIUnit (UIDB/04750/2020). J. Laloy performed STIS at the University of Namur with funding provided by BfR (grant agreement number 1329-561). F. Debacq-Chainiaux is a research associate at FRS-FNRS (National Funds for Scientific Research, Belgium).MDPIRepositório Científico do Instituto Nacional de SaúdeBrandão, FátimaCosta, CarlaBessa, Maria JoãoDumortier, EliseDebacq-Chainiaux, FlorenceHubaux, RolandSalmon, MichelLaloy, JulieStan, Miruna S.Hermenean, AncaGharbia, SamiDinischiotu, AncaBannuscher, AnneHellack, BryanHaase, AndreaFraga, SóniaTeixeira, João2022-02-01T16:25:44Z2021-06-072021-06-07T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.18/7909engNanomaterials (Basel). 2021 Jun 7;11(6):1502. doi: 10.3390/nano110615022079-499110.3390/nano11061502info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-20T15:42:13Zoai:repositorio.insa.pt:10400.18/7909Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:42:27.387799Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Genotoxicity and Gene Expression in the Rat Lung Tissue following Instillation and Inhalation of Different Variants of Amorphous Silica Nanomaterials (aSiO2 NM)
title Genotoxicity and Gene Expression in the Rat Lung Tissue following Instillation and Inhalation of Different Variants of Amorphous Silica Nanomaterials (aSiO2 NM)
spellingShingle Genotoxicity and Gene Expression in the Rat Lung Tissue following Instillation and Inhalation of Different Variants of Amorphous Silica Nanomaterials (aSiO2 NM)
Brandão, Fátima
Toxicology
Silica Nanomaterials
In vivo Inhalation
In vivo Instillation
Rat Lung
DNA Damage
Gene Expression
Nanomaterials
Genotoxicidade Ambiental
title_short Genotoxicity and Gene Expression in the Rat Lung Tissue following Instillation and Inhalation of Different Variants of Amorphous Silica Nanomaterials (aSiO2 NM)
title_full Genotoxicity and Gene Expression in the Rat Lung Tissue following Instillation and Inhalation of Different Variants of Amorphous Silica Nanomaterials (aSiO2 NM)
title_fullStr Genotoxicity and Gene Expression in the Rat Lung Tissue following Instillation and Inhalation of Different Variants of Amorphous Silica Nanomaterials (aSiO2 NM)
title_full_unstemmed Genotoxicity and Gene Expression in the Rat Lung Tissue following Instillation and Inhalation of Different Variants of Amorphous Silica Nanomaterials (aSiO2 NM)
title_sort Genotoxicity and Gene Expression in the Rat Lung Tissue following Instillation and Inhalation of Different Variants of Amorphous Silica Nanomaterials (aSiO2 NM)
author Brandão, Fátima
author_facet Brandão, Fátima
Costa, Carla
Bessa, Maria João
Dumortier, Elise
Debacq-Chainiaux, Florence
Hubaux, Roland
Salmon, Michel
Laloy, Julie
Stan, Miruna S.
Hermenean, Anca
Gharbia, Sami
Dinischiotu, Anca
Bannuscher, Anne
Hellack, Bryan
Haase, Andrea
Fraga, Sónia
Teixeira, João
author_role author
author2 Costa, Carla
Bessa, Maria João
Dumortier, Elise
Debacq-Chainiaux, Florence
Hubaux, Roland
Salmon, Michel
Laloy, Julie
Stan, Miruna S.
Hermenean, Anca
Gharbia, Sami
Dinischiotu, Anca
Bannuscher, Anne
Hellack, Bryan
Haase, Andrea
Fraga, Sónia
Teixeira, João
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Repositório Científico do Instituto Nacional de Saúde
dc.contributor.author.fl_str_mv Brandão, Fátima
Costa, Carla
Bessa, Maria João
Dumortier, Elise
Debacq-Chainiaux, Florence
Hubaux, Roland
Salmon, Michel
Laloy, Julie
Stan, Miruna S.
Hermenean, Anca
Gharbia, Sami
Dinischiotu, Anca
Bannuscher, Anne
Hellack, Bryan
Haase, Andrea
Fraga, Sónia
Teixeira, João
dc.subject.por.fl_str_mv Toxicology
Silica Nanomaterials
In vivo Inhalation
In vivo Instillation
Rat Lung
DNA Damage
Gene Expression
Nanomaterials
Genotoxicidade Ambiental
topic Toxicology
Silica Nanomaterials
In vivo Inhalation
In vivo Instillation
Rat Lung
DNA Damage
Gene Expression
Nanomaterials
Genotoxicidade Ambiental
description This article belongs to the Special Issue Cytotoxicity and Genotoxicity of Nanomaterials.
publishDate 2021
dc.date.none.fl_str_mv 2021-06-07
2021-06-07T00:00:00Z
2022-02-01T16:25:44Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.18/7909
url http://hdl.handle.net/10400.18/7909
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Nanomaterials (Basel). 2021 Jun 7;11(6):1502. doi: 10.3390/nano11061502
2079-4991
10.3390/nano11061502
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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