Genotoxicity of synthetic amorphous silica nanoparticles in rats following short-term exposure. Part 1: Oral route
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.18/3089 |
Resumo: | Synthetic amorphous silica (SAS) in its nanosized form is now used in food applications although the potential risks for human health have not been evaluated. In this study, genotoxicity and oxidative DNA damage of two pyrogenic (NM-202 and 203) and two precipitated (NM-200 and -201) nanosized SAS were investigated in vivo in rats following oral exposure. Male Sprague Dawley rats were exposed to 5, 10, or 20 mg/kg b.w./day for three days by gavage. DNA strand breaks and oxidative DNA damage were investigated in seven tissues (blood, bone marrow from femur, liver,spleen, kidney, duodenum, and colon) with the alkaline and the (Fpg)-modified comet assays, respectively. Concomitantly, chromosomal damage was investigated in bone marrow and in colon with the micronucleus assay. Additionally, malondialdehyde (MDA), a lipid peroxidation marker, was measured in plasma. When required, a histopathological examination was also conducted. The results showed neither obvious DNA strand breaks nor oxidative damage with the comet assay, irrespective of the dose and the organ investigated. Similarly, no increases in chromosome damage in bone marrow or lipid peroxidation in plasma were detected. However, although the response was not dose-dependent, a weak increase in the percentage of micronucleated cells was observed in the colon of rats treated with the two pyrogenic SAS at the lowest dose (5 mg/kg b.w./day). Additional data are required to confirm this result, considering in particular, the role of agglomeration/aggregation of SAS NMs in their uptake by intestinal cells. |
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Genotoxicity of synthetic amorphous silica nanoparticles in rats following short-term exposure. Part 1: Oral routeGenotoxicidade AmbientalGenotoxicitySilica NanomaterialsComet AssayMicronucleus AssayOxidative StressSynthetic amorphous silica (SAS) in its nanosized form is now used in food applications although the potential risks for human health have not been evaluated. In this study, genotoxicity and oxidative DNA damage of two pyrogenic (NM-202 and 203) and two precipitated (NM-200 and -201) nanosized SAS were investigated in vivo in rats following oral exposure. Male Sprague Dawley rats were exposed to 5, 10, or 20 mg/kg b.w./day for three days by gavage. DNA strand breaks and oxidative DNA damage were investigated in seven tissues (blood, bone marrow from femur, liver,spleen, kidney, duodenum, and colon) with the alkaline and the (Fpg)-modified comet assays, respectively. Concomitantly, chromosomal damage was investigated in bone marrow and in colon with the micronucleus assay. Additionally, malondialdehyde (MDA), a lipid peroxidation marker, was measured in plasma. When required, a histopathological examination was also conducted. The results showed neither obvious DNA strand breaks nor oxidative damage with the comet assay, irrespective of the dose and the organ investigated. Similarly, no increases in chromosome damage in bone marrow or lipid peroxidation in plasma were detected. However, although the response was not dose-dependent, a weak increase in the percentage of micronucleated cells was observed in the colon of rats treated with the two pyrogenic SAS at the lowest dose (5 mg/kg b.w./day). Additional data are required to confirm this result, considering in particular, the role of agglomeration/aggregation of SAS NMs in their uptake by intestinal cells.NANOGENOTOX Joint Action program (European Union in the framework of the Health Program and co-funded by the Executive Agency for Health and Consumers, grant agreement 2009 21 01).WileyRepositório Científico do Instituto Nacional de SaúdeTarantini, AdelineHuet, SylvieJarry, GérardMartine, PoulTavares, AnaVital, NádiaLouro, HenriquetaSilva, Maria JoãoFessard, Valérie2015-06-26T13:24:26Z2015-032015-03-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.18/3089porEnviron Mol Mutagen. 2015 Mar;56(2):218-27. doi: 10.1002/em.21935. Epub 2014 Dec 150893-669210.1002/em.21935info:eu-repo/semantics/embargoedAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-20T15:39:36Zoai:repositorio.insa.pt:10400.18/3089Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:38:01.189567Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Genotoxicity of synthetic amorphous silica nanoparticles in rats following short-term exposure. Part 1: Oral route |
title |
Genotoxicity of synthetic amorphous silica nanoparticles in rats following short-term exposure. Part 1: Oral route |
spellingShingle |
Genotoxicity of synthetic amorphous silica nanoparticles in rats following short-term exposure. Part 1: Oral route Tarantini, Adeline Genotoxicidade Ambiental Genotoxicity Silica Nanomaterials Comet Assay Micronucleus Assay Oxidative Stress |
title_short |
Genotoxicity of synthetic amorphous silica nanoparticles in rats following short-term exposure. Part 1: Oral route |
title_full |
Genotoxicity of synthetic amorphous silica nanoparticles in rats following short-term exposure. Part 1: Oral route |
title_fullStr |
Genotoxicity of synthetic amorphous silica nanoparticles in rats following short-term exposure. Part 1: Oral route |
title_full_unstemmed |
Genotoxicity of synthetic amorphous silica nanoparticles in rats following short-term exposure. Part 1: Oral route |
title_sort |
Genotoxicity of synthetic amorphous silica nanoparticles in rats following short-term exposure. Part 1: Oral route |
author |
Tarantini, Adeline |
author_facet |
Tarantini, Adeline Huet, Sylvie Jarry, Gérard Martine, Poul Tavares, Ana Vital, Nádia Louro, Henriqueta Silva, Maria João Fessard, Valérie |
author_role |
author |
author2 |
Huet, Sylvie Jarry, Gérard Martine, Poul Tavares, Ana Vital, Nádia Louro, Henriqueta Silva, Maria João Fessard, Valérie |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Repositório Científico do Instituto Nacional de Saúde |
dc.contributor.author.fl_str_mv |
Tarantini, Adeline Huet, Sylvie Jarry, Gérard Martine, Poul Tavares, Ana Vital, Nádia Louro, Henriqueta Silva, Maria João Fessard, Valérie |
dc.subject.por.fl_str_mv |
Genotoxicidade Ambiental Genotoxicity Silica Nanomaterials Comet Assay Micronucleus Assay Oxidative Stress |
topic |
Genotoxicidade Ambiental Genotoxicity Silica Nanomaterials Comet Assay Micronucleus Assay Oxidative Stress |
description |
Synthetic amorphous silica (SAS) in its nanosized form is now used in food applications although the potential risks for human health have not been evaluated. In this study, genotoxicity and oxidative DNA damage of two pyrogenic (NM-202 and 203) and two precipitated (NM-200 and -201) nanosized SAS were investigated in vivo in rats following oral exposure. Male Sprague Dawley rats were exposed to 5, 10, or 20 mg/kg b.w./day for three days by gavage. DNA strand breaks and oxidative DNA damage were investigated in seven tissues (blood, bone marrow from femur, liver,spleen, kidney, duodenum, and colon) with the alkaline and the (Fpg)-modified comet assays, respectively. Concomitantly, chromosomal damage was investigated in bone marrow and in colon with the micronucleus assay. Additionally, malondialdehyde (MDA), a lipid peroxidation marker, was measured in plasma. When required, a histopathological examination was also conducted. The results showed neither obvious DNA strand breaks nor oxidative damage with the comet assay, irrespective of the dose and the organ investigated. Similarly, no increases in chromosome damage in bone marrow or lipid peroxidation in plasma were detected. However, although the response was not dose-dependent, a weak increase in the percentage of micronucleated cells was observed in the colon of rats treated with the two pyrogenic SAS at the lowest dose (5 mg/kg b.w./day). Additional data are required to confirm this result, considering in particular, the role of agglomeration/aggregation of SAS NMs in their uptake by intestinal cells. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-06-26T13:24:26Z 2015-03 2015-03-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.18/3089 |
url |
http://hdl.handle.net/10400.18/3089 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
Environ Mol Mutagen. 2015 Mar;56(2):218-27. doi: 10.1002/em.21935. Epub 2014 Dec 15 0893-6692 10.1002/em.21935 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/embargoedAccess |
eu_rights_str_mv |
embargoedAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Wiley |
publisher.none.fl_str_mv |
Wiley |
dc.source.none.fl_str_mv |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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