Antibody isotype epitope mapping of SARS-CoV-2 Spike RBD protein
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2023 |
| Outros Autores: | , , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| Texto Completo: | http://hdl.handle.net/10362/154354 |
Resumo: | Funding Information: This study was supported by Ministerio de Ciencia e Innovación/Agencia Estatal de Investigación, Spain and EU‐FEDER [Grant BIOGAL PID2020‐116761GB‐I00]. M.C. was funded by the Ministerio de Ciencia, Innovación y Universidades, Spain, grant IJC2020‐042710‐I. RV‐R was funded by Universidad de Castilla‐La Mancha (UCLM), Spain and the European Social Fund (ESF) [grant 2022‐PRED‐20675]. Funding Information: This study was supported by Ministerio de Ciencia e Innovación/Agencia Estatal de Investigación, Spain and EU-FEDER [Grant BIOGAL PID2020-116761GB-I00]. M.C. was funded by the Ministerio de Ciencia, Innovación y Universidades, Spain, grant IJC2020-042710-I. RV-R was funded by Universidad de Castilla-La Mancha (UCLM), Spain and the European Social Fund (ESF) [grant 2022-PRED-20675]. Publisher Copyright: © 2023 The Authors. European Journal of Immunology published by Wiley-VCH GmbH. |
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Antibody isotype epitope mapping of SARS-CoV-2 Spike RBD proteinTargets for COVID-19 symptomatology and disease controlantibody isotypeepitope mappingprognostic, SARS-CoV-2spike proteinRA0421 Public health. Hygiene. Preventive MedicineQR355 VirologyQR MicrobiologyQR180 ImmunologyInfectious DiseasesImmunology and AllergyImmunologySDG 3 - Good Health and Well-beingSDG 9 - Industry, Innovation, and InfrastructureFunding Information: This study was supported by Ministerio de Ciencia e Innovación/Agencia Estatal de Investigación, Spain and EU‐FEDER [Grant BIOGAL PID2020‐116761GB‐I00]. M.C. was funded by the Ministerio de Ciencia, Innovación y Universidades, Spain, grant IJC2020‐042710‐I. RV‐R was funded by Universidad de Castilla‐La Mancha (UCLM), Spain and the European Social Fund (ESF) [grant 2022‐PRED‐20675]. Funding Information: This study was supported by Ministerio de Ciencia e Innovación/Agencia Estatal de Investigación, Spain and EU-FEDER [Grant BIOGAL PID2020-116761GB-I00]. M.C. was funded by the Ministerio de Ciencia, Innovación y Universidades, Spain, grant IJC2020-042710-I. RV-R was funded by Universidad de Castilla-La Mancha (UCLM), Spain and the European Social Fund (ESF) [grant 2022-PRED-20675]. Publisher Copyright: © 2023 The Authors. European Journal of Immunology published by Wiley-VCH GmbH.Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) still poses a challenge for biomedicine and public health. To advance the development of effective diagnostic, prognostic, and preventive interventions, our study focused on high-throughput antibody binding epitope mapping of the SARS-CoV-2 spike RBD protein by IgA, IgM and IgG antibodies in saliva and sera of different cohorts from healthy uninfected individuals to SARS-CoV-2-infected unvaccinated and vaccinated asymptomatic, recovered, nonsevere, and severe patients. Identified candidate diagnostic (455-LFRKSNLKPFERD-467), prognostic (395-VYADSFVIRGDEV-407-C-KLH, 332-ITNLCPFGEV-342-C-KLH, 352-AWNRKRI-358-C-KLH, 524-VCGPKKSTNLVKN-536-KLH), and protective (MKLLE-487-NCYFPLQSYGFQPTNGVG-504-GGGGS-446-GGNYNYLYRLFRKSNLKPFERD-467) epitopes were validated with sera from prevaccine and postvaccine cohorts. The results identified neutralizing epitopes and support that antibody recognition of linear B-cell epitopes in RBD protein is associated with antibody isotype and disease symptomatology. The findings in asymptomatic individuals suggest a role for anti-RBD antibodies in the protective response against SARS-CoV-2. The possibility of translating results into diagnostic interventions for the early diagnosis of asymptomatic individuals and prognosis of disease severity provides new tools for COVID-19 surveillance and evaluation of risks in hospitalized patients. These results, together with other approaches, may contribute to the development of new vaccines for the control of COVID-19 and other coronavirus-related diseases using a quantum vaccinomics approach through the combination of protective epitopes.Instituto de Higiene e Medicina Tropical (IHMT)Vector borne diseases and pathogens (VBD)Global Health and Tropical Medicine (GHTM)RUNContreras, MarinelaVicente, JoaquínCerón, José JoaquínMartinez Subiela, SilviaUrra, José MiguelRodríguez-del-Río, Francisco J.Ferreras-Colino, ElisaVaz-Rodrigues, Ritade Fernández de Mera, Isabel G.Antunes, SandraDomingos, AnaGortázar, Christiande la Fuente, José2023-06-23T22:24:15Z2023-042023-04-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article17application/pdfhttp://hdl.handle.net/10362/154354eng0014-2980PURE: 64220256https://doi.org/10.1002/eji.202250206info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T05:36:48Zoai:run.unl.pt:10362/154354Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:55:35.666829Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
| dc.title.none.fl_str_mv |
Antibody isotype epitope mapping of SARS-CoV-2 Spike RBD protein Targets for COVID-19 symptomatology and disease control |
| title |
Antibody isotype epitope mapping of SARS-CoV-2 Spike RBD protein |
| spellingShingle |
Antibody isotype epitope mapping of SARS-CoV-2 Spike RBD protein Contreras, Marinela antibody isotype epitope mapping prognostic, SARS-CoV-2 spike protein RA0421 Public health. Hygiene. Preventive Medicine QR355 Virology QR Microbiology QR180 Immunology Infectious Diseases Immunology and Allergy Immunology SDG 3 - Good Health and Well-being SDG 9 - Industry, Innovation, and Infrastructure |
| title_short |
Antibody isotype epitope mapping of SARS-CoV-2 Spike RBD protein |
| title_full |
Antibody isotype epitope mapping of SARS-CoV-2 Spike RBD protein |
| title_fullStr |
Antibody isotype epitope mapping of SARS-CoV-2 Spike RBD protein |
| title_full_unstemmed |
Antibody isotype epitope mapping of SARS-CoV-2 Spike RBD protein |
| title_sort |
Antibody isotype epitope mapping of SARS-CoV-2 Spike RBD protein |
| author |
Contreras, Marinela |
| author_facet |
Contreras, Marinela Vicente, Joaquín Cerón, José Joaquín Martinez Subiela, Silvia Urra, José Miguel Rodríguez-del-Río, Francisco J. Ferreras-Colino, Elisa Vaz-Rodrigues, Rita de Fernández de Mera, Isabel G. Antunes, Sandra Domingos, Ana Gortázar, Christian de la Fuente, José |
| author_role |
author |
| author2 |
Vicente, Joaquín Cerón, José Joaquín Martinez Subiela, Silvia Urra, José Miguel Rodríguez-del-Río, Francisco J. Ferreras-Colino, Elisa Vaz-Rodrigues, Rita de Fernández de Mera, Isabel G. Antunes, Sandra Domingos, Ana Gortázar, Christian de la Fuente, José |
| author2_role |
author author author author author author author author author author author author |
| dc.contributor.none.fl_str_mv |
Instituto de Higiene e Medicina Tropical (IHMT) Vector borne diseases and pathogens (VBD) Global Health and Tropical Medicine (GHTM) RUN |
| dc.contributor.author.fl_str_mv |
Contreras, Marinela Vicente, Joaquín Cerón, José Joaquín Martinez Subiela, Silvia Urra, José Miguel Rodríguez-del-Río, Francisco J. Ferreras-Colino, Elisa Vaz-Rodrigues, Rita de Fernández de Mera, Isabel G. Antunes, Sandra Domingos, Ana Gortázar, Christian de la Fuente, José |
| dc.subject.por.fl_str_mv |
antibody isotype epitope mapping prognostic, SARS-CoV-2 spike protein RA0421 Public health. Hygiene. Preventive Medicine QR355 Virology QR Microbiology QR180 Immunology Infectious Diseases Immunology and Allergy Immunology SDG 3 - Good Health and Well-being SDG 9 - Industry, Innovation, and Infrastructure |
| topic |
antibody isotype epitope mapping prognostic, SARS-CoV-2 spike protein RA0421 Public health. Hygiene. Preventive Medicine QR355 Virology QR Microbiology QR180 Immunology Infectious Diseases Immunology and Allergy Immunology SDG 3 - Good Health and Well-being SDG 9 - Industry, Innovation, and Infrastructure |
| description |
Funding Information: This study was supported by Ministerio de Ciencia e Innovación/Agencia Estatal de Investigación, Spain and EU‐FEDER [Grant BIOGAL PID2020‐116761GB‐I00]. M.C. was funded by the Ministerio de Ciencia, Innovación y Universidades, Spain, grant IJC2020‐042710‐I. RV‐R was funded by Universidad de Castilla‐La Mancha (UCLM), Spain and the European Social Fund (ESF) [grant 2022‐PRED‐20675]. Funding Information: This study was supported by Ministerio de Ciencia e Innovación/Agencia Estatal de Investigación, Spain and EU-FEDER [Grant BIOGAL PID2020-116761GB-I00]. M.C. was funded by the Ministerio de Ciencia, Innovación y Universidades, Spain, grant IJC2020-042710-I. RV-R was funded by Universidad de Castilla-La Mancha (UCLM), Spain and the European Social Fund (ESF) [grant 2022-PRED-20675]. Publisher Copyright: © 2023 The Authors. European Journal of Immunology published by Wiley-VCH GmbH. |
| publishDate |
2023 |
| dc.date.none.fl_str_mv |
2023-06-23T22:24:15Z 2023-04 2023-04-01T00:00:00Z |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
| dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10362/154354 |
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http://hdl.handle.net/10362/154354 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
0014-2980 PURE: 64220256 https://doi.org/10.1002/eji.202250206 |
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info:eu-repo/semantics/openAccess |
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openAccess |
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17 application/pdf |
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