Biophysical characterization of polymyxin B interaction with LPS aggregates and membrane model systems
Autor(a) principal: | |
---|---|
Data de Publicação: | 2012 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10451/10757 |
Resumo: | © 2012 Wiley Periodicals, Inc. |
id |
RCAP_aef1b8be5a403264509bea1b10e36ab4 |
---|---|
oai_identifier_str |
oai:repositorio.ul.pt:10451/10757 |
network_acronym_str |
RCAP |
network_name_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository_id_str |
7160 |
spelling |
Biophysical characterization of polymyxin B interaction with LPS aggregates and membrane model systemsPolymyxin BLipopolysaccharideMembrane model systems© 2012 Wiley Periodicals, Inc.Antibiotic resistance is an increasingly severe health problem. Antimicrobial peptides (AMPs) are being developed in order to overcome this problem, due to their lower bacterial resistance. Polymyxin B is an AMP with bactericidal effect on Gram-negative bacteria due to its high affinity for lipopolysaccharide (LPS). The objective of this work was to unravel the polymyxin B mechanisms of LPS neutralization and bactericidal activity. Using dynamic light scattering, it was observed that polymyxin B induces LPS aggregation in a concentration-dependent manner. The peptide increases the surface charge of LPS and membrane model systems, as revealed by zetapotential measurements. The higher zeta-potential variations were detected in the presence of the negatively charged POPG membranes. This higher interaction with negatively charged membranes, made of POPG, was followed at higher peptide concentration by membrane permeabilization. Also, for zwitterionic POPC membranes a higher membrane leakage was detected. The peptide promotion of LPS aggregation may be related with the clearance of LPS from the bloodstream, eventually by facilitating macrophage phagocytosis and/or blocking the binding of LPS to its receptor. Our data indicate that polymyxin B mechanism of action at the molecular level involves a first step of electrostatic approach toward LPS; then, it may be internalized and bind to the bacterial phosphatidylglycerol-rich membrane leaflets, inducing leakage at higher peptide concentrations.Contract grant sponsor: Fundação para a Ciência e a Tecnologia – Ministério do Ensino e Ciência (FCT-MES, Portugal). Contract grant number: PTDC/SAU-BEB/099142/2008. Contract grant sponsors: Gabinete de Apoio à Investigação Científica, Tecnológica e Inovação (GAPIC), of the Faculdade de Medicina da Universidade de Lisboa. Contract grant sponsor: FCT-MÊS. Contract grant number: SFRH/BD/41750/2007.WileyRepositório da Universidade de LisboaDomingues, Marco M.Inácio, Rita G.Raimundo, José M.Martins, MiguelCastanho, Miguel A. R. B.Santos, Nuno C.2014-03-21T10:17:24Z20122012-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10451/10757engPeptideScience, Volume 98 / Number 40006-3525http://dx.doi.org/10.1002/bip.22095metadata only accessinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-08T15:56:28Zoai:repositorio.ul.pt:10451/10757Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T21:34:40.887100Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Biophysical characterization of polymyxin B interaction with LPS aggregates and membrane model systems |
title |
Biophysical characterization of polymyxin B interaction with LPS aggregates and membrane model systems |
spellingShingle |
Biophysical characterization of polymyxin B interaction with LPS aggregates and membrane model systems Domingues, Marco M. Polymyxin B Lipopolysaccharide Membrane model systems |
title_short |
Biophysical characterization of polymyxin B interaction with LPS aggregates and membrane model systems |
title_full |
Biophysical characterization of polymyxin B interaction with LPS aggregates and membrane model systems |
title_fullStr |
Biophysical characterization of polymyxin B interaction with LPS aggregates and membrane model systems |
title_full_unstemmed |
Biophysical characterization of polymyxin B interaction with LPS aggregates and membrane model systems |
title_sort |
Biophysical characterization of polymyxin B interaction with LPS aggregates and membrane model systems |
author |
Domingues, Marco M. |
author_facet |
Domingues, Marco M. Inácio, Rita G. Raimundo, José M. Martins, Miguel Castanho, Miguel A. R. B. Santos, Nuno C. |
author_role |
author |
author2 |
Inácio, Rita G. Raimundo, José M. Martins, Miguel Castanho, Miguel A. R. B. Santos, Nuno C. |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Repositório da Universidade de Lisboa |
dc.contributor.author.fl_str_mv |
Domingues, Marco M. Inácio, Rita G. Raimundo, José M. Martins, Miguel Castanho, Miguel A. R. B. Santos, Nuno C. |
dc.subject.por.fl_str_mv |
Polymyxin B Lipopolysaccharide Membrane model systems |
topic |
Polymyxin B Lipopolysaccharide Membrane model systems |
description |
© 2012 Wiley Periodicals, Inc. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012 2012-01-01T00:00:00Z 2014-03-21T10:17:24Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10451/10757 |
url |
http://hdl.handle.net/10451/10757 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
PeptideScience, Volume 98 / Number 4 0006-3525 http://dx.doi.org/10.1002/bip.22095 |
dc.rights.driver.fl_str_mv |
metadata only access info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
metadata only access |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Wiley |
publisher.none.fl_str_mv |
Wiley |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
|
_version_ |
1799134241645133824 |