Fluorescence spectroscopy and chemometrics for simultaneous monitoring of cell concentration, chlorophyll and fatty acids in Nannochloropsis oceanica
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2020 |
| Outros Autores: | , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| Texto Completo: | http://hdl.handle.net/10362/105367 |
Resumo: | Associate Laboratory for Green Chemistry - LAQV which is financed by national funds from FCT/MCTES (UID/QUI/50006/2019), PhD grant SFRH/BD/108894/2015 of FCT/MCTES. |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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7160 |
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Fluorescence spectroscopy and chemometrics for simultaneous monitoring of cell concentration, chlorophyll and fatty acids in Nannochloropsis oceanicaGeneralAssociate Laboratory for Green Chemistry - LAQV which is financed by national funds from FCT/MCTES (UID/QUI/50006/2019), PhD grant SFRH/BD/108894/2015 of FCT/MCTES.Online monitoring of algal biotechnological processes still requires development to support economic sustainability. In this work, fluorescence spectroscopy coupled with chemometric modelling is studied to monitor simultaneously several compounds of interest, such as chlorophyll and fatty acids, but also the biomass as a whole (cell concentration). Fluorescence excitation-emission matrices (EEM) were acquired in experiments where different environmental growing parameters were tested, namely light regime, temperature and nitrogen (replete or deplete medium). The prediction models developed have a high R2 for the validation data set for all five parameters monitored, specifically cell concentration (0.66), chlorophyll (0.78), and fatty acid as total (0.78), saturated (0.81) and unsaturated (0.74). Regression coefficient maps of the models show the importance of the pigment region for all outputs studied, and the protein-like fluorescence region for the cell concentration. These results demonstrate for the first time the potential of fluorescence spectroscopy for in vivo and real-time monitoring of these key performance parameters during Nannochloropsis oceanica cultivation.LAQV@REQUIMTEDQ - Departamento de QuímicaRUNSá, MartaBertinetto, Carlo G.Ferrer-Ledo, NarcísJansen, Jeroen J.Wijffels, ReneCrespo, João G.Barbosa, MariaGalinha, Claudia F.2020-10-09T22:49:55Z2020-12-012020-12-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/105367eng2045-2322PURE: 20056080https://doi.org/10.1038/s41598-020-64628-7info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-05-22T17:48:00Zoai:run.unl.pt:10362/105367Portal AgregadorONGhttps://www.rcaap.pt/oai/openairemluisa.alvim@gmail.comopendoar:71602024-05-22T17:48Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
| dc.title.none.fl_str_mv |
Fluorescence spectroscopy and chemometrics for simultaneous monitoring of cell concentration, chlorophyll and fatty acids in Nannochloropsis oceanica |
| title |
Fluorescence spectroscopy and chemometrics for simultaneous monitoring of cell concentration, chlorophyll and fatty acids in Nannochloropsis oceanica |
| spellingShingle |
Fluorescence spectroscopy and chemometrics for simultaneous monitoring of cell concentration, chlorophyll and fatty acids in Nannochloropsis oceanica Sá, Marta General |
| title_short |
Fluorescence spectroscopy and chemometrics for simultaneous monitoring of cell concentration, chlorophyll and fatty acids in Nannochloropsis oceanica |
| title_full |
Fluorescence spectroscopy and chemometrics for simultaneous monitoring of cell concentration, chlorophyll and fatty acids in Nannochloropsis oceanica |
| title_fullStr |
Fluorescence spectroscopy and chemometrics for simultaneous monitoring of cell concentration, chlorophyll and fatty acids in Nannochloropsis oceanica |
| title_full_unstemmed |
Fluorescence spectroscopy and chemometrics for simultaneous monitoring of cell concentration, chlorophyll and fatty acids in Nannochloropsis oceanica |
| title_sort |
Fluorescence spectroscopy and chemometrics for simultaneous monitoring of cell concentration, chlorophyll and fatty acids in Nannochloropsis oceanica |
| author |
Sá, Marta |
| author_facet |
Sá, Marta Bertinetto, Carlo G. Ferrer-Ledo, Narcís Jansen, Jeroen J. Wijffels, Rene Crespo, João G. Barbosa, Maria Galinha, Claudia F. |
| author_role |
author |
| author2 |
Bertinetto, Carlo G. Ferrer-Ledo, Narcís Jansen, Jeroen J. Wijffels, Rene Crespo, João G. Barbosa, Maria Galinha, Claudia F. |
| author2_role |
author author author author author author author |
| dc.contributor.none.fl_str_mv |
LAQV@REQUIMTE DQ - Departamento de Química RUN |
| dc.contributor.author.fl_str_mv |
Sá, Marta Bertinetto, Carlo G. Ferrer-Ledo, Narcís Jansen, Jeroen J. Wijffels, Rene Crespo, João G. Barbosa, Maria Galinha, Claudia F. |
| dc.subject.por.fl_str_mv |
General |
| topic |
General |
| description |
Associate Laboratory for Green Chemistry - LAQV which is financed by national funds from FCT/MCTES (UID/QUI/50006/2019), PhD grant SFRH/BD/108894/2015 of FCT/MCTES. |
| publishDate |
2020 |
| dc.date.none.fl_str_mv |
2020-10-09T22:49:55Z 2020-12-01 2020-12-01T00:00:00Z |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10362/105367 |
| url |
http://hdl.handle.net/10362/105367 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
2045-2322 PURE: 20056080 https://doi.org/10.1038/s41598-020-64628-7 |
| dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
| eu_rights_str_mv |
openAccess |
| dc.format.none.fl_str_mv |
application/pdf |
| dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
| instacron_str |
RCAAP |
| institution |
RCAAP |
| reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
| repository.mail.fl_str_mv |
mluisa.alvim@gmail.com |
| _version_ |
1817545761420738560 |