Production of sophorolipds with a branched hydrophobic tail
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Tipo de documento: | Dissertação |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10773/15310 |
Resumo: | Sophorolipids are a type of biosurfactants produced by several microorganisms, including the yeast Starmerella bombicola. They find application mainly due to their emulsifying activity and antimicrobial properties. Sophorolipids produced by Starmerella bombicola are composed by one sophorose molecule and a hydroxylated fatty acid of 16 or 18 carbon atoms. One or two acetylations can occur, one on each glucose, and a lactonization as well. Despite the interesting characteristics of classic sophorolipids produced by the referred yeast, there are indications that sophorolipids with branched a hydrophobic tail have higher activity at lower temperatures. These sophorolipids are produced by Rhodotorula bogoriensis, however the yields obtained with this yeast are low. By producing these sophorolipids in Starmerella bombicola, the yields obtained could be higher. Furthermore, the production of these novel sophorolipids will allow broadening of the biosurfactants applications. This thesis describes two approaches to produce sophorolipids with a branched hydrophobic tail: use of Guerbet alcohols as substrates for the Starmerella bombicola culture, and the introduction of genes that will take care of the in-chain hydroxylation of the sophorolipids. Guerbet alcohols are branched molecules, and are expected to be incorporated in the sophorolipids molecules. The genes to be used are from Elizabethkingia meningoseptica and Rhodococcus rhodochrous and encode for an oleate hydratase, an enzyme responsible for the conversion of oleic acid into 10-hydroxystearic acid. |
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Production of sophorolipds with a branched hydrophobic tailBiotecnologiaLevedurasBiosurfactantesStarmerella bombicolaSophorolipids are a type of biosurfactants produced by several microorganisms, including the yeast Starmerella bombicola. They find application mainly due to their emulsifying activity and antimicrobial properties. Sophorolipids produced by Starmerella bombicola are composed by one sophorose molecule and a hydroxylated fatty acid of 16 or 18 carbon atoms. One or two acetylations can occur, one on each glucose, and a lactonization as well. Despite the interesting characteristics of classic sophorolipids produced by the referred yeast, there are indications that sophorolipids with branched a hydrophobic tail have higher activity at lower temperatures. These sophorolipids are produced by Rhodotorula bogoriensis, however the yields obtained with this yeast are low. By producing these sophorolipids in Starmerella bombicola, the yields obtained could be higher. Furthermore, the production of these novel sophorolipids will allow broadening of the biosurfactants applications. This thesis describes two approaches to produce sophorolipids with a branched hydrophobic tail: use of Guerbet alcohols as substrates for the Starmerella bombicola culture, and the introduction of genes that will take care of the in-chain hydroxylation of the sophorolipids. Guerbet alcohols are branched molecules, and are expected to be incorporated in the sophorolipids molecules. The genes to be used are from Elizabethkingia meningoseptica and Rhodococcus rhodochrous and encode for an oleate hydratase, an enzyme responsible for the conversion of oleic acid into 10-hydroxystearic acid.Soforolípidos são um tipo de biosurfactantes produzidos por vários microorganismos, incluindo a levedura Starmerella bombicola. As suas principais aplicações devem-se à sua sua atividade emulsificante e propriedades antimicrobianas. Soforolípidos produzidos por Starmerella bombicola são compostos por uma molécula de soforose e um ácido gordo hidroxilado de 16 ou 18 átomos de carbono. Apesar das interessantes caraterísticas dos soforolípidos clássicos produzidos pela levedura referida, há indicações de que os soforolípidos com cauda hidrofóbica ramificada têm maior atividade a temperaturas mais elevadas. Estes soforolípidos são produzidos por Rhodotorula bogoriensis, no entanto o rendimento obtido por esta levedura é baixo. Ao produzir estes soforolípidos em Starmerella bombicola, o rendimento obtido poderá ser superior. A produção destes soforolípidos irá também permitir o alargamento das aplicações dos biosurfactantes. Esta tese descreve duas medidas para a produção de soforolípidos com cauda hidrofóbica ramificada: uso de álcoois de Guerbet como substrato para a cultura de Starmerella bombicola, e a introdução de genes que serão responsáveis pela hidroxilação no meio da cadeia hidrofóbica dos soforolípidos. Álcoois de Guerbet são moléculas ramificadas, sendo esperado a sua incorporação nas moléculas de soforolípidos. Os genes usados são provenientes de Elizabethkingia meningoseptica e Rhodococcus rhodochrous, e codificam para uma oleate hidratase, enzima responsável pela conversão do ácido oleico no ácido 10-hidroxistearico.Universidade de Aveiro2018-07-20T14:00:52Z2015-07-16T00:00:00Z2015-07-162017-07-16T18:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfhttp://hdl.handle.net/10773/15310engTeixeira, Liliana Patrícia dos Reisinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-02-22T11:28:19Zoai:ria.ua.pt:10773/15310Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T02:50:42.336934Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Production of sophorolipds with a branched hydrophobic tail |
title |
Production of sophorolipds with a branched hydrophobic tail |
spellingShingle |
Production of sophorolipds with a branched hydrophobic tail Teixeira, Liliana Patrícia dos Reis Biotecnologia Leveduras Biosurfactantes Starmerella bombicola |
title_short |
Production of sophorolipds with a branched hydrophobic tail |
title_full |
Production of sophorolipds with a branched hydrophobic tail |
title_fullStr |
Production of sophorolipds with a branched hydrophobic tail |
title_full_unstemmed |
Production of sophorolipds with a branched hydrophobic tail |
title_sort |
Production of sophorolipds with a branched hydrophobic tail |
author |
Teixeira, Liliana Patrícia dos Reis |
author_facet |
Teixeira, Liliana Patrícia dos Reis |
author_role |
author |
dc.contributor.author.fl_str_mv |
Teixeira, Liliana Patrícia dos Reis |
dc.subject.por.fl_str_mv |
Biotecnologia Leveduras Biosurfactantes Starmerella bombicola |
topic |
Biotecnologia Leveduras Biosurfactantes Starmerella bombicola |
description |
Sophorolipids are a type of biosurfactants produced by several microorganisms, including the yeast Starmerella bombicola. They find application mainly due to their emulsifying activity and antimicrobial properties. Sophorolipids produced by Starmerella bombicola are composed by one sophorose molecule and a hydroxylated fatty acid of 16 or 18 carbon atoms. One or two acetylations can occur, one on each glucose, and a lactonization as well. Despite the interesting characteristics of classic sophorolipids produced by the referred yeast, there are indications that sophorolipids with branched a hydrophobic tail have higher activity at lower temperatures. These sophorolipids are produced by Rhodotorula bogoriensis, however the yields obtained with this yeast are low. By producing these sophorolipids in Starmerella bombicola, the yields obtained could be higher. Furthermore, the production of these novel sophorolipids will allow broadening of the biosurfactants applications. This thesis describes two approaches to produce sophorolipids with a branched hydrophobic tail: use of Guerbet alcohols as substrates for the Starmerella bombicola culture, and the introduction of genes that will take care of the in-chain hydroxylation of the sophorolipids. Guerbet alcohols are branched molecules, and are expected to be incorporated in the sophorolipids molecules. The genes to be used are from Elizabethkingia meningoseptica and Rhodococcus rhodochrous and encode for an oleate hydratase, an enzyme responsible for the conversion of oleic acid into 10-hydroxystearic acid. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-07-16T00:00:00Z 2015-07-16 2017-07-16T18:00:00Z 2018-07-20T14:00:52Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10773/15310 |
url |
http://hdl.handle.net/10773/15310 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade de Aveiro |
publisher.none.fl_str_mv |
Universidade de Aveiro |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799137557228814336 |