Characterization of the cytochrome system of a nitrogen‐fixing strain of a sulfate‐reducing bacterium: Desulfovibrio desulfuricans strain Berre‐Eau
Autor(a) principal: | |
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Data de Publicação: | 1987 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | https://doi.org/10.1111/j.1432-1033.1987.tb10674.x |
Resumo: | Two c‐type cytochromes were purified and characterized by electron paramagnetic resonance (EPR) and nuclear magnetic resonance (NMR) spectroscopic techniques, from the sulfate‐reducer nitrogen‐fixing organism, Desulfovibrio desulfuricans strain Berre‐Eau (NCIB 8387). The purification procedures included several chromatographic steps on alumina, carboxymethylcellulose and gel filtration. A tetrahaem and a monohaem cytochrome were identified. The multihaem cytochrome has visible, EPR and NMR spectra with general properties similar to other low‐potential bis‐histidinyl axially bound haem proteins, belonging to the class of tetrahaem cytochrome c3 isolated from other Desulfovibrio species. The monohaem cytochrome c553 is ascorbate‐reducible and its EPR and NMR data are characteristic of a cytochrome with methionine‐histidine ligation. Their properties are compared with other homologous proteins isolated from sulfate‐reducing bacteria. |
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Characterization of the cytochrome system of a nitrogen‐fixing strain of a sulfate‐reducing bacterium: Desulfovibrio desulfuricans strain Berre‐EauBiochemistryTwo c‐type cytochromes were purified and characterized by electron paramagnetic resonance (EPR) and nuclear magnetic resonance (NMR) spectroscopic techniques, from the sulfate‐reducer nitrogen‐fixing organism, Desulfovibrio desulfuricans strain Berre‐Eau (NCIB 8387). The purification procedures included several chromatographic steps on alumina, carboxymethylcellulose and gel filtration. A tetrahaem and a monohaem cytochrome were identified. The multihaem cytochrome has visible, EPR and NMR spectra with general properties similar to other low‐potential bis‐histidinyl axially bound haem proteins, belonging to the class of tetrahaem cytochrome c3 isolated from other Desulfovibrio species. The monohaem cytochrome c553 is ascorbate‐reducible and its EPR and NMR data are characteristic of a cytochrome with methionine‐histidine ligation. Their properties are compared with other homologous proteins isolated from sulfate‐reducing bacteria.DQ - Departamento de QuímicaUniversidade NOVA de LisboaRUNMoura, IsabelFauque, GuyLeGall, JeanXavier, António V.Moura, José J. G.2019-09-11T22:44:37Z1987-01-011987-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article8application/pdfhttps://doi.org/10.1111/j.1432-1033.1987.tb10674.xeng0014-2956PURE: 14656075http://www.scopus.com/inward/record.url?scp=0023131491&partnerID=8YFLogxKhttps://doi.org/10.1111/j.1432-1033.1987.tb10674.xinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T04:35:53Zoai:run.unl.pt:10362/80902Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:35:58.162429Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Characterization of the cytochrome system of a nitrogen‐fixing strain of a sulfate‐reducing bacterium: Desulfovibrio desulfuricans strain Berre‐Eau |
title |
Characterization of the cytochrome system of a nitrogen‐fixing strain of a sulfate‐reducing bacterium: Desulfovibrio desulfuricans strain Berre‐Eau |
spellingShingle |
Characterization of the cytochrome system of a nitrogen‐fixing strain of a sulfate‐reducing bacterium: Desulfovibrio desulfuricans strain Berre‐Eau Moura, Isabel Biochemistry |
title_short |
Characterization of the cytochrome system of a nitrogen‐fixing strain of a sulfate‐reducing bacterium: Desulfovibrio desulfuricans strain Berre‐Eau |
title_full |
Characterization of the cytochrome system of a nitrogen‐fixing strain of a sulfate‐reducing bacterium: Desulfovibrio desulfuricans strain Berre‐Eau |
title_fullStr |
Characterization of the cytochrome system of a nitrogen‐fixing strain of a sulfate‐reducing bacterium: Desulfovibrio desulfuricans strain Berre‐Eau |
title_full_unstemmed |
Characterization of the cytochrome system of a nitrogen‐fixing strain of a sulfate‐reducing bacterium: Desulfovibrio desulfuricans strain Berre‐Eau |
title_sort |
Characterization of the cytochrome system of a nitrogen‐fixing strain of a sulfate‐reducing bacterium: Desulfovibrio desulfuricans strain Berre‐Eau |
author |
Moura, Isabel |
author_facet |
Moura, Isabel Fauque, Guy LeGall, Jean Xavier, António V. Moura, José J. G. |
author_role |
author |
author2 |
Fauque, Guy LeGall, Jean Xavier, António V. Moura, José J. G. |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
DQ - Departamento de Química Universidade NOVA de Lisboa RUN |
dc.contributor.author.fl_str_mv |
Moura, Isabel Fauque, Guy LeGall, Jean Xavier, António V. Moura, José J. G. |
dc.subject.por.fl_str_mv |
Biochemistry |
topic |
Biochemistry |
description |
Two c‐type cytochromes were purified and characterized by electron paramagnetic resonance (EPR) and nuclear magnetic resonance (NMR) spectroscopic techniques, from the sulfate‐reducer nitrogen‐fixing organism, Desulfovibrio desulfuricans strain Berre‐Eau (NCIB 8387). The purification procedures included several chromatographic steps on alumina, carboxymethylcellulose and gel filtration. A tetrahaem and a monohaem cytochrome were identified. The multihaem cytochrome has visible, EPR and NMR spectra with general properties similar to other low‐potential bis‐histidinyl axially bound haem proteins, belonging to the class of tetrahaem cytochrome c3 isolated from other Desulfovibrio species. The monohaem cytochrome c553 is ascorbate‐reducible and its EPR and NMR data are characteristic of a cytochrome with methionine‐histidine ligation. Their properties are compared with other homologous proteins isolated from sulfate‐reducing bacteria. |
publishDate |
1987 |
dc.date.none.fl_str_mv |
1987-01-01 1987-01-01T00:00:00Z 2019-09-11T22:44:37Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://doi.org/10.1111/j.1432-1033.1987.tb10674.x |
url |
https://doi.org/10.1111/j.1432-1033.1987.tb10674.x |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
0014-2956 PURE: 14656075 http://www.scopus.com/inward/record.url?scp=0023131491&partnerID=8YFLogxK https://doi.org/10.1111/j.1432-1033.1987.tb10674.x |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
8 application/pdf |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799137979696939008 |