Characterization of the Thermostable Biosurfactant Produced by Burkholderia thailandensis DSM 13276
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2022 |
| Outros Autores: | , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| Texto Completo: | http://hdl.handle.net/10362/145493 |
Resumo: | project LA/P/0140/2020 of the Associate Laboratory Institute for Health and Bioeconomy—i4HB; National Natural Science Foundation of China (grant numbers: Institute of Microbiology, Chinese Academy of Sciences: 31961133016; Beijing Institute of Technology: 31961133015; Shandong University: 31961133014). C.V.G., A.E. and A.T.R. acknowledge FCT I.P. for the Ph.D. grants 2021.05798.BD, 2021.05014.BD and 2020.06470.BD, respectively. Publisher Copyright: © 2022 by the authors. Licensee MDPI, Basel, Switzerland. |
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Characterization of the Thermostable Biosurfactant Produced by Burkholderia thailandensis DSM 13276BiosurfactantsEmulsion stabilityRheologyThermostabilityChemistry(all)Polymers and Plasticsproject LA/P/0140/2020 of the Associate Laboratory Institute for Health and Bioeconomy—i4HB; National Natural Science Foundation of China (grant numbers: Institute of Microbiology, Chinese Academy of Sciences: 31961133016; Beijing Institute of Technology: 31961133015; Shandong University: 31961133014). C.V.G., A.E. and A.T.R. acknowledge FCT I.P. for the Ph.D. grants 2021.05798.BD, 2021.05014.BD and 2020.06470.BD, respectively. Publisher Copyright: © 2022 by the authors. Licensee MDPI, Basel, Switzerland.Biosurfactants synthesized by microorganisms represent safe and sustainable alternatives to the use of synthetic surfactants, due to their lower toxicity, better biodegradability and biocompatibility, and their production from low-cost feedstocks. In line with this, the present study describes the physical, chemical, and functional characterization of the biopolymer secreted by the bacterium Burkholderia thailandensis DSM 13276, envisaging its validation as a biosurfactant. The biopolymer was found to be a glycolipopeptide with carbohydrate and protein contents of 33.1 ± 6.4% and 23.0 ± 3.2%, respectively. Galactose, glucose, rhamnose, mannose, and glucuronic acid were detected in the carbohydrate moiety at a relative molar ratio of 4:3:2:2:1. It is a high-molecular-weight biopolymer (1.0×107Da) with low polydispersity (1.66), and forms aqueous solutions with shear-thinning behavior, which remained after autoclaving. The biopolymer has demonstrated a good emulsionstabilizing capacity towards different hydrophobic compounds, namely, benzene, almond oil, and sunflower oil. The emulsions prepared with the biosurfactant, as well as with its autoclaved solution, displayed high emulsification activity (>90% and ~50%, respectively). Moreover, the almond and sunflower oil emulsions stabilized with the biosurfactant were stable for up to 4 weeks, which further supports the potential of this novel biopolymer for utilization as a natural bioemulsifier.DQ - Departamento de QuímicaUCIBIO - Applied Molecular Biosciences UnitRUNGil, Cátia V.Rebocho, Ana TeresaEsmail, AsiyahSevrin, ChantalGrandfils, ChristianTorres, Cristiana A.V.Reis, Maria A.M.Freitas, Filomena2022-11-14T22:09:46Z2022-05-202022-05-20T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article12application/pdfhttp://hdl.handle.net/10362/145493engPURE: 47509176https://doi.org/10.3390/polym14102088info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T05:25:54Zoai:run.unl.pt:10362/145493Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:52:06.355249Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
| dc.title.none.fl_str_mv |
Characterization of the Thermostable Biosurfactant Produced by Burkholderia thailandensis DSM 13276 |
| title |
Characterization of the Thermostable Biosurfactant Produced by Burkholderia thailandensis DSM 13276 |
| spellingShingle |
Characterization of the Thermostable Biosurfactant Produced by Burkholderia thailandensis DSM 13276 Gil, Cátia V. Biosurfactants Emulsion stability Rheology Thermostability Chemistry(all) Polymers and Plastics |
| title_short |
Characterization of the Thermostable Biosurfactant Produced by Burkholderia thailandensis DSM 13276 |
| title_full |
Characterization of the Thermostable Biosurfactant Produced by Burkholderia thailandensis DSM 13276 |
| title_fullStr |
Characterization of the Thermostable Biosurfactant Produced by Burkholderia thailandensis DSM 13276 |
| title_full_unstemmed |
Characterization of the Thermostable Biosurfactant Produced by Burkholderia thailandensis DSM 13276 |
| title_sort |
Characterization of the Thermostable Biosurfactant Produced by Burkholderia thailandensis DSM 13276 |
| author |
Gil, Cátia V. |
| author_facet |
Gil, Cátia V. Rebocho, Ana Teresa Esmail, Asiyah Sevrin, Chantal Grandfils, Christian Torres, Cristiana A.V. Reis, Maria A.M. Freitas, Filomena |
| author_role |
author |
| author2 |
Rebocho, Ana Teresa Esmail, Asiyah Sevrin, Chantal Grandfils, Christian Torres, Cristiana A.V. Reis, Maria A.M. Freitas, Filomena |
| author2_role |
author author author author author author author |
| dc.contributor.none.fl_str_mv |
DQ - Departamento de Química UCIBIO - Applied Molecular Biosciences Unit RUN |
| dc.contributor.author.fl_str_mv |
Gil, Cátia V. Rebocho, Ana Teresa Esmail, Asiyah Sevrin, Chantal Grandfils, Christian Torres, Cristiana A.V. Reis, Maria A.M. Freitas, Filomena |
| dc.subject.por.fl_str_mv |
Biosurfactants Emulsion stability Rheology Thermostability Chemistry(all) Polymers and Plastics |
| topic |
Biosurfactants Emulsion stability Rheology Thermostability Chemistry(all) Polymers and Plastics |
| description |
project LA/P/0140/2020 of the Associate Laboratory Institute for Health and Bioeconomy—i4HB; National Natural Science Foundation of China (grant numbers: Institute of Microbiology, Chinese Academy of Sciences: 31961133016; Beijing Institute of Technology: 31961133015; Shandong University: 31961133014). C.V.G., A.E. and A.T.R. acknowledge FCT I.P. for the Ph.D. grants 2021.05798.BD, 2021.05014.BD and 2020.06470.BD, respectively. Publisher Copyright: © 2022 by the authors. Licensee MDPI, Basel, Switzerland. |
| publishDate |
2022 |
| dc.date.none.fl_str_mv |
2022-11-14T22:09:46Z 2022-05-20 2022-05-20T00:00:00Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
| dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10362/145493 |
| url |
http://hdl.handle.net/10362/145493 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
PURE: 47509176 https://doi.org/10.3390/polym14102088 |
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info:eu-repo/semantics/openAccess |
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openAccess |
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12 application/pdf |
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reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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