In vitro tests of suitability of bacteriocin-producing lactic acid bacteria, as potential biopreservation cultures in vacuum-packaged cold-smoked salmon

Detalhes bibliográficos
Autor(a) principal: Tomé, Elisabetta
Data de Publicação: 2008
Outros Autores: Pereira, Vera L., Lopes, Carla I., Gibbs, Paul A., Teixeira, Paula C.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.14/2742
Resumo: The objectives of this study were to ascertain the in vitro potential of nine bacteriocin-producing lactic acid bacteria (LAB) strains, isolated from vacuum-packaged cold-smoked salmon (CSS), for possible use as biopreservative cultures against Listeria monocytogenes. The antilisterial activity of cultures’ supernatants was assessed at 0.5%, 3.0% and 5.0% w/v of salt, at 5ºC, 10ºC and 25ºC both in aerobic and anaerobic atmospheres, simulating the conditions pertaining in vacuum-packaged CSS. The kinetics of growth, bacteriocin biosynthesis, the minimum inhibitory concentrations (MICs) of ten antibiotics, histamine and tyramine production of LAB strains, were determined jointly with the haemolytic activity for the enterococci. Only five strains were able to secrete active bacteriocins into the culture medium, at high salt concentrations and low temperatures, both in aerobic and anaerobic atmospheres. Enterococci showed neither haemolyt ic activity nor vancomycin resistance. The production of histamine was not observed for any of the bacteriocin-producing strains.
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spelling In vitro tests of suitability of bacteriocin-producing lactic acid bacteria, as potential biopreservation cultures in vacuum-packaged cold-smoked salmonCold-smoked fishSalmonBacteriocinsListeriaEnterococcusLactobacillusPediococcusThe objectives of this study were to ascertain the in vitro potential of nine bacteriocin-producing lactic acid bacteria (LAB) strains, isolated from vacuum-packaged cold-smoked salmon (CSS), for possible use as biopreservative cultures against Listeria monocytogenes. The antilisterial activity of cultures’ supernatants was assessed at 0.5%, 3.0% and 5.0% w/v of salt, at 5ºC, 10ºC and 25ºC both in aerobic and anaerobic atmospheres, simulating the conditions pertaining in vacuum-packaged CSS. The kinetics of growth, bacteriocin biosynthesis, the minimum inhibitory concentrations (MICs) of ten antibiotics, histamine and tyramine production of LAB strains, were determined jointly with the haemolytic activity for the enterococci. Only five strains were able to secrete active bacteriocins into the culture medium, at high salt concentrations and low temperatures, both in aerobic and anaerobic atmospheres. Enterococci showed neither haemolyt ic activity nor vancomycin resistance. The production of histamine was not observed for any of the bacteriocin-producing strains.ElsevierVeritati - Repositório Institucional da Universidade Católica PortuguesaTomé, ElisabettaPereira, Vera L.Lopes, Carla I.Gibbs, Paul A.Teixeira, Paula C.2010-10-08T17:37:44Z20082008-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.14/2742eng"Food Control". ISSN 0956-7135. 19: 5 (2008) 535–543info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-09-06T11:58:56Zoai:repositorio.ucp.pt:10400.14/2742Portal AgregadorONGhttps://www.rcaap.pt/oai/openairemluisa.alvim@gmail.comopendoar:71602024-09-06T11:58:56Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv In vitro tests of suitability of bacteriocin-producing lactic acid bacteria, as potential biopreservation cultures in vacuum-packaged cold-smoked salmon
title In vitro tests of suitability of bacteriocin-producing lactic acid bacteria, as potential biopreservation cultures in vacuum-packaged cold-smoked salmon
spellingShingle In vitro tests of suitability of bacteriocin-producing lactic acid bacteria, as potential biopreservation cultures in vacuum-packaged cold-smoked salmon
Tomé, Elisabetta
Cold-smoked fish
Salmon
Bacteriocins
Listeria
Enterococcus
Lactobacillus
Pediococcus
title_short In vitro tests of suitability of bacteriocin-producing lactic acid bacteria, as potential biopreservation cultures in vacuum-packaged cold-smoked salmon
title_full In vitro tests of suitability of bacteriocin-producing lactic acid bacteria, as potential biopreservation cultures in vacuum-packaged cold-smoked salmon
title_fullStr In vitro tests of suitability of bacteriocin-producing lactic acid bacteria, as potential biopreservation cultures in vacuum-packaged cold-smoked salmon
title_full_unstemmed In vitro tests of suitability of bacteriocin-producing lactic acid bacteria, as potential biopreservation cultures in vacuum-packaged cold-smoked salmon
title_sort In vitro tests of suitability of bacteriocin-producing lactic acid bacteria, as potential biopreservation cultures in vacuum-packaged cold-smoked salmon
author Tomé, Elisabetta
author_facet Tomé, Elisabetta
Pereira, Vera L.
Lopes, Carla I.
Gibbs, Paul A.
Teixeira, Paula C.
author_role author
author2 Pereira, Vera L.
Lopes, Carla I.
Gibbs, Paul A.
Teixeira, Paula C.
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Veritati - Repositório Institucional da Universidade Católica Portuguesa
dc.contributor.author.fl_str_mv Tomé, Elisabetta
Pereira, Vera L.
Lopes, Carla I.
Gibbs, Paul A.
Teixeira, Paula C.
dc.subject.por.fl_str_mv Cold-smoked fish
Salmon
Bacteriocins
Listeria
Enterococcus
Lactobacillus
Pediococcus
topic Cold-smoked fish
Salmon
Bacteriocins
Listeria
Enterococcus
Lactobacillus
Pediococcus
description The objectives of this study were to ascertain the in vitro potential of nine bacteriocin-producing lactic acid bacteria (LAB) strains, isolated from vacuum-packaged cold-smoked salmon (CSS), for possible use as biopreservative cultures against Listeria monocytogenes. The antilisterial activity of cultures’ supernatants was assessed at 0.5%, 3.0% and 5.0% w/v of salt, at 5ºC, 10ºC and 25ºC both in aerobic and anaerobic atmospheres, simulating the conditions pertaining in vacuum-packaged CSS. The kinetics of growth, bacteriocin biosynthesis, the minimum inhibitory concentrations (MICs) of ten antibiotics, histamine and tyramine production of LAB strains, were determined jointly with the haemolytic activity for the enterococci. Only five strains were able to secrete active bacteriocins into the culture medium, at high salt concentrations and low temperatures, both in aerobic and anaerobic atmospheres. Enterococci showed neither haemolyt ic activity nor vancomycin resistance. The production of histamine was not observed for any of the bacteriocin-producing strains.
publishDate 2008
dc.date.none.fl_str_mv 2008
2008-01-01T00:00:00Z
2010-10-08T17:37:44Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.14/2742
url http://hdl.handle.net/10400.14/2742
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv "Food Control". ISSN 0956-7135. 19: 5 (2008) 535–543
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv mluisa.alvim@gmail.com
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