In vitro tests of suitability of bacteriocin-producing lactic acid bacteria, as potential biopreservation cultures in vacuum-packaged cold-smoked salmon
Autor(a) principal: | |
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Data de Publicação: | 2008 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.14/2742 |
Resumo: | The objectives of this study were to ascertain the in vitro potential of nine bacteriocin-producing lactic acid bacteria (LAB) strains, isolated from vacuum-packaged cold-smoked salmon (CSS), for possible use as biopreservative cultures against Listeria monocytogenes. The antilisterial activity of cultures’ supernatants was assessed at 0.5%, 3.0% and 5.0% w/v of salt, at 5ºC, 10ºC and 25ºC both in aerobic and anaerobic atmospheres, simulating the conditions pertaining in vacuum-packaged CSS. The kinetics of growth, bacteriocin biosynthesis, the minimum inhibitory concentrations (MICs) of ten antibiotics, histamine and tyramine production of LAB strains, were determined jointly with the haemolytic activity for the enterococci. Only five strains were able to secrete active bacteriocins into the culture medium, at high salt concentrations and low temperatures, both in aerobic and anaerobic atmospheres. Enterococci showed neither haemolyt ic activity nor vancomycin resistance. The production of histamine was not observed for any of the bacteriocin-producing strains. |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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7160 |
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In vitro tests of suitability of bacteriocin-producing lactic acid bacteria, as potential biopreservation cultures in vacuum-packaged cold-smoked salmonCold-smoked fishSalmonBacteriocinsListeriaEnterococcusLactobacillusPediococcusThe objectives of this study were to ascertain the in vitro potential of nine bacteriocin-producing lactic acid bacteria (LAB) strains, isolated from vacuum-packaged cold-smoked salmon (CSS), for possible use as biopreservative cultures against Listeria monocytogenes. The antilisterial activity of cultures’ supernatants was assessed at 0.5%, 3.0% and 5.0% w/v of salt, at 5ºC, 10ºC and 25ºC both in aerobic and anaerobic atmospheres, simulating the conditions pertaining in vacuum-packaged CSS. The kinetics of growth, bacteriocin biosynthesis, the minimum inhibitory concentrations (MICs) of ten antibiotics, histamine and tyramine production of LAB strains, were determined jointly with the haemolytic activity for the enterococci. Only five strains were able to secrete active bacteriocins into the culture medium, at high salt concentrations and low temperatures, both in aerobic and anaerobic atmospheres. Enterococci showed neither haemolyt ic activity nor vancomycin resistance. The production of histamine was not observed for any of the bacteriocin-producing strains.ElsevierVeritati - Repositório Institucional da Universidade Católica PortuguesaTomé, ElisabettaPereira, Vera L.Lopes, Carla I.Gibbs, Paul A.Teixeira, Paula C.2010-10-08T17:37:44Z20082008-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.14/2742eng"Food Control". ISSN 0956-7135. 19: 5 (2008) 535–543info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-09-06T11:58:56Zoai:repositorio.ucp.pt:10400.14/2742Portal AgregadorONGhttps://www.rcaap.pt/oai/openairemluisa.alvim@gmail.comopendoar:71602024-09-06T11:58:56Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
In vitro tests of suitability of bacteriocin-producing lactic acid bacteria, as potential biopreservation cultures in vacuum-packaged cold-smoked salmon |
title |
In vitro tests of suitability of bacteriocin-producing lactic acid bacteria, as potential biopreservation cultures in vacuum-packaged cold-smoked salmon |
spellingShingle |
In vitro tests of suitability of bacteriocin-producing lactic acid bacteria, as potential biopreservation cultures in vacuum-packaged cold-smoked salmon Tomé, Elisabetta Cold-smoked fish Salmon Bacteriocins Listeria Enterococcus Lactobacillus Pediococcus |
title_short |
In vitro tests of suitability of bacteriocin-producing lactic acid bacteria, as potential biopreservation cultures in vacuum-packaged cold-smoked salmon |
title_full |
In vitro tests of suitability of bacteriocin-producing lactic acid bacteria, as potential biopreservation cultures in vacuum-packaged cold-smoked salmon |
title_fullStr |
In vitro tests of suitability of bacteriocin-producing lactic acid bacteria, as potential biopreservation cultures in vacuum-packaged cold-smoked salmon |
title_full_unstemmed |
In vitro tests of suitability of bacteriocin-producing lactic acid bacteria, as potential biopreservation cultures in vacuum-packaged cold-smoked salmon |
title_sort |
In vitro tests of suitability of bacteriocin-producing lactic acid bacteria, as potential biopreservation cultures in vacuum-packaged cold-smoked salmon |
author |
Tomé, Elisabetta |
author_facet |
Tomé, Elisabetta Pereira, Vera L. Lopes, Carla I. Gibbs, Paul A. Teixeira, Paula C. |
author_role |
author |
author2 |
Pereira, Vera L. Lopes, Carla I. Gibbs, Paul A. Teixeira, Paula C. |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Veritati - Repositório Institucional da Universidade Católica Portuguesa |
dc.contributor.author.fl_str_mv |
Tomé, Elisabetta Pereira, Vera L. Lopes, Carla I. Gibbs, Paul A. Teixeira, Paula C. |
dc.subject.por.fl_str_mv |
Cold-smoked fish Salmon Bacteriocins Listeria Enterococcus Lactobacillus Pediococcus |
topic |
Cold-smoked fish Salmon Bacteriocins Listeria Enterococcus Lactobacillus Pediococcus |
description |
The objectives of this study were to ascertain the in vitro potential of nine bacteriocin-producing lactic acid bacteria (LAB) strains, isolated from vacuum-packaged cold-smoked salmon (CSS), for possible use as biopreservative cultures against Listeria monocytogenes. The antilisterial activity of cultures’ supernatants was assessed at 0.5%, 3.0% and 5.0% w/v of salt, at 5ºC, 10ºC and 25ºC both in aerobic and anaerobic atmospheres, simulating the conditions pertaining in vacuum-packaged CSS. The kinetics of growth, bacteriocin biosynthesis, the minimum inhibitory concentrations (MICs) of ten antibiotics, histamine and tyramine production of LAB strains, were determined jointly with the haemolytic activity for the enterococci. Only five strains were able to secrete active bacteriocins into the culture medium, at high salt concentrations and low temperatures, both in aerobic and anaerobic atmospheres. Enterococci showed neither haemolyt ic activity nor vancomycin resistance. The production of histamine was not observed for any of the bacteriocin-producing strains. |
publishDate |
2008 |
dc.date.none.fl_str_mv |
2008 2008-01-01T00:00:00Z 2010-10-08T17:37:44Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.14/2742 |
url |
http://hdl.handle.net/10400.14/2742 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
"Food Control". ISSN 0956-7135. 19: 5 (2008) 535–543 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
mluisa.alvim@gmail.com |
_version_ |
1817546696047984640 |