A bacteriophage detection tool for viability assessment of Salmonella cells
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/27313 |
Resumo: | Available online 7 September 2013 |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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7160 |
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A bacteriophage detection tool for viability assessment of Salmonella cellsBacteriophageSalmonellaCell viabilityViable but non-culturable bacteriaMagnetoresistive biochipMagnetic nanoparticlesScience & TechnologyAvailable online 7 September 2013Salmonellosis, one of the most common food and water-borne diseases, has a major global health and economic impact. Salmonella cells present high infection rates, persistence over inauspicious conditions and the potential to preserve virulence in dormant states when cells are viable but non-culturable (VBNC). These facts are challenging for current detection methods. Culture methods lack the capacity to detect VBNC cells, while biomolecular methods (e.g. DNA- or protein-based) hardly distinguish between dead innocuous cells and their viable lethal counterparts. This work presents and validates a novel bacteriophage (phage)-based microbial detection tool to detect and assess Salmonella viability. Salmonella Enteritidis cells in a VBNC physiological state were evaluated by cell culture, flow-cytometry and epifluorescence microscopy, and further assayed with a biosensor platform. Free PVP-SE1 phages in solution showed the ability to recognize VBNC cells, with no lysis induction, in contrast to the minor recognition of heat-killed cells. This ability was confirmed for immobilized phages on gold surfaces, where the phage detection signal follows the same trend of the concentration of viable plus VBNC cells in the sample. The phage probe was then tested in a magnetoresistive biosensor platform allowing the quantitative detection and discrimination of viable and VBNC cells from dead cells, with high sensitivity. Signals arising from 3 to 4 cells per sensor were recorded. In comparison to a polyclonal antibody that does not distinguish viable from dead cells, the phage selectivity in cell recognition minimizes false-negative and false-positive results often associated with most detection methods.ElsevierUniversidade do MinhoFernandes, Elisabete RamosMartins, V. C.Nóbrega, CláudiaCarvalho, Carla A. O. C. M.Cardoso, F. A.Cardoso, S.Dias, J.Deng, D.Kluskens, LeonFreitas, P. P.Azeredo, Joana20142014-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/27313eng0956-566310.1016/j.bios.2013.08.05324055938http://www.sciencedirect.com/science/article/pii/S0956566313006027info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:20:27Zoai:repositorium.sdum.uminho.pt:1822/27313Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:13:34.592937Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
A bacteriophage detection tool for viability assessment of Salmonella cells |
title |
A bacteriophage detection tool for viability assessment of Salmonella cells |
spellingShingle |
A bacteriophage detection tool for viability assessment of Salmonella cells Fernandes, Elisabete Ramos Bacteriophage Salmonella Cell viability Viable but non-culturable bacteria Magnetoresistive biochip Magnetic nanoparticles Science & Technology |
title_short |
A bacteriophage detection tool for viability assessment of Salmonella cells |
title_full |
A bacteriophage detection tool for viability assessment of Salmonella cells |
title_fullStr |
A bacteriophage detection tool for viability assessment of Salmonella cells |
title_full_unstemmed |
A bacteriophage detection tool for viability assessment of Salmonella cells |
title_sort |
A bacteriophage detection tool for viability assessment of Salmonella cells |
author |
Fernandes, Elisabete Ramos |
author_facet |
Fernandes, Elisabete Ramos Martins, V. C. Nóbrega, Cláudia Carvalho, Carla A. O. C. M. Cardoso, F. A. Cardoso, S. Dias, J. Deng, D. Kluskens, Leon Freitas, P. P. Azeredo, Joana |
author_role |
author |
author2 |
Martins, V. C. Nóbrega, Cláudia Carvalho, Carla A. O. C. M. Cardoso, F. A. Cardoso, S. Dias, J. Deng, D. Kluskens, Leon Freitas, P. P. Azeredo, Joana |
author2_role |
author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Fernandes, Elisabete Ramos Martins, V. C. Nóbrega, Cláudia Carvalho, Carla A. O. C. M. Cardoso, F. A. Cardoso, S. Dias, J. Deng, D. Kluskens, Leon Freitas, P. P. Azeredo, Joana |
dc.subject.por.fl_str_mv |
Bacteriophage Salmonella Cell viability Viable but non-culturable bacteria Magnetoresistive biochip Magnetic nanoparticles Science & Technology |
topic |
Bacteriophage Salmonella Cell viability Viable but non-culturable bacteria Magnetoresistive biochip Magnetic nanoparticles Science & Technology |
description |
Available online 7 September 2013 |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014 2014-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/27313 |
url |
http://hdl.handle.net/1822/27313 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
0956-5663 10.1016/j.bios.2013.08.053 24055938 http://www.sciencedirect.com/science/article/pii/S0956566313006027 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
|
_version_ |
1799132574568677376 |