Microbial lipids from industrial wastes using xylose-utilizing Ashbya gossypii strains

Detalhes bibliográficos
Autor(a) principal: Díaz-Fernández, David
Data de Publicação: 2019
Outros Autores: Aguiar, Tatiana Quinta, Martín, Victoria Isabel, Romani, Aloia Perez, Silva, Rui, Domingues, Lucília, Revuelta, José Luis, Jiménez, Alberto
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/61335
Resumo: Supplementary data to this article can be found online at https:// doi.org/10.1016/j.biortech.2019.122054.
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spelling Microbial lipids from industrial wastes using xylose-utilizing Ashbya gossypii strainsAshbya gossypiimicrobial lipidsregulationlignocellulosic hydrolysatesxylosemolassesglycerolScience & TechnologySupplementary data to this article can be found online at https:// doi.org/10.1016/j.biortech.2019.122054.This work presents the exploitation of waste industrial by-products as raw materials for the production of microbial lipids in engineered strains of the filamentous fungus Ashbya gossypii. A lipogenic xylose-utilizing strain was used to apply a metabolic engineering approach aiming at relieving regulatory mechanisms to further increase the biosynthesis of lipids. Three genomic manipulations were applied: the overexpression of a feedback resistant form of the acetyl-CoA carboxylase enzyme; the expression of a truncated form of Mga2, a regulator of the main 9 desaturase gene; and the overexpression of an additional copy of DGA1 that codes for diacylglycerol acyltransferase. The performance of the engineered strain was evaluated in culture media containing mixed formulations of corn-cob hydrolysates, sugarcane molasses or crude glycerol. Our results demonstrate the efficiency of the engineered strains, which were able to accumulate about 40% of cell dry weight (CDW) in lipid content using organic industrial wastes as feedstocks.This work was supported by: the Spanish Ministerio de Economía y Competitividad (grant numbers BIO2014-56930-PandBIO2017-88435R) and the Junta de Castilla y León (grant number SA016P17); the Portuguese Foundation for Science and Technology (FCT) under the scope of the strategic funding of UID/BIO/04469/2019, PhD grant PD/ BD/113812/2015 to R. Silva (Doctoral Program in Applied and Environmental Microbiology), the MultiBiorefinery project (POCI-010145-FEDER-016403) and the BioTecNorte operation (NORTE-010145-FEDER-000004) funded by the European Regional Development Fund under the scope of Norte2020 - Programa Operacional Regional do Norte. DDF was recipient of USAL predoctoral fellowship from the University of Salamanca.info:eu-repo/semantics/publishedVersionElsevierUniversidade do MinhoDíaz-Fernández, DavidAguiar, Tatiana QuintaMartín, Victoria IsabelRomani, Aloia PerezSilva, RuiDomingues, LucíliaRevuelta, José LuisJiménez, Alberto2019-122019-12-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/61335engDíaz-Fernández, David; Aguiar, Tatiana Q.; Martín, Victoria Isabel; Romaní, Aloia; Silva, Rui; Domingues, Lucília; Revuelta, José Luis; Jiménez, Alberto, Microbial lipids from industrial wastes using xylose-utilizing Ashbya gossypii strains. Bioresource Technology, 293(122054), 20190960-85240960-852410.1016/j.biortech.2019.12205431487616http://www.journals.elsevier.com/bioresource-technology/info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:08:44Zoai:repositorium.sdum.uminho.pt:1822/61335Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:59:59.642445Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Microbial lipids from industrial wastes using xylose-utilizing Ashbya gossypii strains
title Microbial lipids from industrial wastes using xylose-utilizing Ashbya gossypii strains
spellingShingle Microbial lipids from industrial wastes using xylose-utilizing Ashbya gossypii strains
Díaz-Fernández, David
Ashbya gossypii
microbial lipids
regulation
lignocellulosic hydrolysates
xylose
molasses
glycerol
Science & Technology
title_short Microbial lipids from industrial wastes using xylose-utilizing Ashbya gossypii strains
title_full Microbial lipids from industrial wastes using xylose-utilizing Ashbya gossypii strains
title_fullStr Microbial lipids from industrial wastes using xylose-utilizing Ashbya gossypii strains
title_full_unstemmed Microbial lipids from industrial wastes using xylose-utilizing Ashbya gossypii strains
title_sort Microbial lipids from industrial wastes using xylose-utilizing Ashbya gossypii strains
author Díaz-Fernández, David
author_facet Díaz-Fernández, David
Aguiar, Tatiana Quinta
Martín, Victoria Isabel
Romani, Aloia Perez
Silva, Rui
Domingues, Lucília
Revuelta, José Luis
Jiménez, Alberto
author_role author
author2 Aguiar, Tatiana Quinta
Martín, Victoria Isabel
Romani, Aloia Perez
Silva, Rui
Domingues, Lucília
Revuelta, José Luis
Jiménez, Alberto
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Díaz-Fernández, David
Aguiar, Tatiana Quinta
Martín, Victoria Isabel
Romani, Aloia Perez
Silva, Rui
Domingues, Lucília
Revuelta, José Luis
Jiménez, Alberto
dc.subject.por.fl_str_mv Ashbya gossypii
microbial lipids
regulation
lignocellulosic hydrolysates
xylose
molasses
glycerol
Science & Technology
topic Ashbya gossypii
microbial lipids
regulation
lignocellulosic hydrolysates
xylose
molasses
glycerol
Science & Technology
description Supplementary data to this article can be found online at https:// doi.org/10.1016/j.biortech.2019.122054.
publishDate 2019
dc.date.none.fl_str_mv 2019-12
2019-12-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/61335
url http://hdl.handle.net/1822/61335
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Díaz-Fernández, David; Aguiar, Tatiana Q.; Martín, Victoria Isabel; Romaní, Aloia; Silva, Rui; Domingues, Lucília; Revuelta, José Luis; Jiménez, Alberto, Microbial lipids from industrial wastes using xylose-utilizing Ashbya gossypii strains. Bioresource Technology, 293(122054), 2019
0960-8524
0960-8524
10.1016/j.biortech.2019.122054
31487616
http://www.journals.elsevier.com/bioresource-technology/
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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