Microbial lipids from industrial wastes using xylose-utilizing Ashbya gossypii strains
Autor(a) principal: | |
---|---|
Data de Publicação: | 2019 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/61335 |
Resumo: | Supplementary data to this article can be found online at https:// doi.org/10.1016/j.biortech.2019.122054. |
id |
RCAP_b4e96c86f32536c784ec614f572772f6 |
---|---|
oai_identifier_str |
oai:repositorium.sdum.uminho.pt:1822/61335 |
network_acronym_str |
RCAP |
network_name_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository_id_str |
7160 |
spelling |
Microbial lipids from industrial wastes using xylose-utilizing Ashbya gossypii strainsAshbya gossypiimicrobial lipidsregulationlignocellulosic hydrolysatesxylosemolassesglycerolScience & TechnologySupplementary data to this article can be found online at https:// doi.org/10.1016/j.biortech.2019.122054.This work presents the exploitation of waste industrial by-products as raw materials for the production of microbial lipids in engineered strains of the filamentous fungus Ashbya gossypii. A lipogenic xylose-utilizing strain was used to apply a metabolic engineering approach aiming at relieving regulatory mechanisms to further increase the biosynthesis of lipids. Three genomic manipulations were applied: the overexpression of a feedback resistant form of the acetyl-CoA carboxylase enzyme; the expression of a truncated form of Mga2, a regulator of the main 9 desaturase gene; and the overexpression of an additional copy of DGA1 that codes for diacylglycerol acyltransferase. The performance of the engineered strain was evaluated in culture media containing mixed formulations of corn-cob hydrolysates, sugarcane molasses or crude glycerol. Our results demonstrate the efficiency of the engineered strains, which were able to accumulate about 40% of cell dry weight (CDW) in lipid content using organic industrial wastes as feedstocks.This work was supported by: the Spanish Ministerio de Economía y Competitividad (grant numbers BIO2014-56930-PandBIO2017-88435R) and the Junta de Castilla y León (grant number SA016P17); the Portuguese Foundation for Science and Technology (FCT) under the scope of the strategic funding of UID/BIO/04469/2019, PhD grant PD/ BD/113812/2015 to R. Silva (Doctoral Program in Applied and Environmental Microbiology), the MultiBiorefinery project (POCI-010145-FEDER-016403) and the BioTecNorte operation (NORTE-010145-FEDER-000004) funded by the European Regional Development Fund under the scope of Norte2020 - Programa Operacional Regional do Norte. DDF was recipient of USAL predoctoral fellowship from the University of Salamanca.info:eu-repo/semantics/publishedVersionElsevierUniversidade do MinhoDíaz-Fernández, DavidAguiar, Tatiana QuintaMartín, Victoria IsabelRomani, Aloia PerezSilva, RuiDomingues, LucíliaRevuelta, José LuisJiménez, Alberto2019-122019-12-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/61335engDíaz-Fernández, David; Aguiar, Tatiana Q.; Martín, Victoria Isabel; Romaní, Aloia; Silva, Rui; Domingues, Lucília; Revuelta, José Luis; Jiménez, Alberto, Microbial lipids from industrial wastes using xylose-utilizing Ashbya gossypii strains. Bioresource Technology, 293(122054), 20190960-85240960-852410.1016/j.biortech.2019.12205431487616http://www.journals.elsevier.com/bioresource-technology/info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:08:44Zoai:repositorium.sdum.uminho.pt:1822/61335Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:59:59.642445Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Microbial lipids from industrial wastes using xylose-utilizing Ashbya gossypii strains |
title |
Microbial lipids from industrial wastes using xylose-utilizing Ashbya gossypii strains |
spellingShingle |
Microbial lipids from industrial wastes using xylose-utilizing Ashbya gossypii strains Díaz-Fernández, David Ashbya gossypii microbial lipids regulation lignocellulosic hydrolysates xylose molasses glycerol Science & Technology |
title_short |
Microbial lipids from industrial wastes using xylose-utilizing Ashbya gossypii strains |
title_full |
Microbial lipids from industrial wastes using xylose-utilizing Ashbya gossypii strains |
title_fullStr |
Microbial lipids from industrial wastes using xylose-utilizing Ashbya gossypii strains |
title_full_unstemmed |
Microbial lipids from industrial wastes using xylose-utilizing Ashbya gossypii strains |
title_sort |
Microbial lipids from industrial wastes using xylose-utilizing Ashbya gossypii strains |
author |
Díaz-Fernández, David |
author_facet |
Díaz-Fernández, David Aguiar, Tatiana Quinta Martín, Victoria Isabel Romani, Aloia Perez Silva, Rui Domingues, Lucília Revuelta, José Luis Jiménez, Alberto |
author_role |
author |
author2 |
Aguiar, Tatiana Quinta Martín, Victoria Isabel Romani, Aloia Perez Silva, Rui Domingues, Lucília Revuelta, José Luis Jiménez, Alberto |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Díaz-Fernández, David Aguiar, Tatiana Quinta Martín, Victoria Isabel Romani, Aloia Perez Silva, Rui Domingues, Lucília Revuelta, José Luis Jiménez, Alberto |
dc.subject.por.fl_str_mv |
Ashbya gossypii microbial lipids regulation lignocellulosic hydrolysates xylose molasses glycerol Science & Technology |
topic |
Ashbya gossypii microbial lipids regulation lignocellulosic hydrolysates xylose molasses glycerol Science & Technology |
description |
Supplementary data to this article can be found online at https:// doi.org/10.1016/j.biortech.2019.122054. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-12 2019-12-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/61335 |
url |
http://hdl.handle.net/1822/61335 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Díaz-Fernández, David; Aguiar, Tatiana Q.; Martín, Victoria Isabel; Romaní, Aloia; Silva, Rui; Domingues, Lucília; Revuelta, José Luis; Jiménez, Alberto, Microbial lipids from industrial wastes using xylose-utilizing Ashbya gossypii strains. Bioresource Technology, 293(122054), 2019 0960-8524 0960-8524 10.1016/j.biortech.2019.122054 31487616 http://www.journals.elsevier.com/bioresource-technology/ |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
|
_version_ |
1799132393176563712 |