Induction of lymphocytes activated marker CD69 following exposure to chitosan and alginate biopolymers
Autor(a) principal: | |
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Data de Publicação: | 2007 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10316/5858 https://doi.org/10.1016/j.ijpharm.2007.01.021 |
Resumo: | CD69 is a very early cell activation antigen expressed on the surface of activated immune cells. It can appear within 1-2 h of activation and exhibits maximal expression levels between 18 and 24 h after stimulation. In this work, the expression profile of CD69 in mice splenocytes was evaluated following exposure to the biopolymers, chitosan or alginate and the immunostimulatory factors, CpG ODN 1826 or concanavalin A. We have shown that both polymers are able to upregulate expression of CD69 on B cells and CD4+ T-lymphocytes, with alginate as the least potent stimulus. Moreover, the expression of the CD69 molecule on CD8+ T-lymphocytes was observed only in splenocytes cultured with chitosan. However, activation of lymphocytes did not result in cell proliferation. On the other hand, CpG ODN proved to be a potent B cell stimulator, as evidenced by the upregulation of CD69, but had less effect on T-cells. These results, together with previous discoveries reported in scientific literature, may contribute to the clarification of the adjuvant effect, which has been attributed to chitosan and alginate formulations or to the biopolymers itself. |
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Induction of lymphocytes activated marker CD69 following exposure to chitosan and alginate biopolymersCD69 expressionChitosanAlginateCpG ODNLymphocyte activationCD69 is a very early cell activation antigen expressed on the surface of activated immune cells. It can appear within 1-2 h of activation and exhibits maximal expression levels between 18 and 24 h after stimulation. In this work, the expression profile of CD69 in mice splenocytes was evaluated following exposure to the biopolymers, chitosan or alginate and the immunostimulatory factors, CpG ODN 1826 or concanavalin A. We have shown that both polymers are able to upregulate expression of CD69 on B cells and CD4+ T-lymphocytes, with alginate as the least potent stimulus. Moreover, the expression of the CD69 molecule on CD8+ T-lymphocytes was observed only in splenocytes cultured with chitosan. However, activation of lymphocytes did not result in cell proliferation. On the other hand, CpG ODN proved to be a potent B cell stimulator, as evidenced by the upregulation of CD69, but had less effect on T-cells. These results, together with previous discoveries reported in scientific literature, may contribute to the clarification of the adjuvant effect, which has been attributed to chitosan and alginate formulations or to the biopolymers itself.http://www.sciencedirect.com/science/article/B6T7W-4MVVSPF-3/1/25d9e02017b2e01767337d0c5f17efcc2007info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleaplication/PDFhttp://hdl.handle.net/10316/5858http://hdl.handle.net/10316/5858https://doi.org/10.1016/j.ijpharm.2007.01.021engInternational Journal of Pharmaceutics. 337:1-2 (2007) 254-264Borges, OlgaBorchard, GerritSousa, Adriano deJunginger, Hans E.Cordeiro-da-Silva, Anabelainfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2022-05-25T03:10:44Zoai:estudogeral.uc.pt:10316/5858Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T20:47:24.899193Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Induction of lymphocytes activated marker CD69 following exposure to chitosan and alginate biopolymers |
title |
Induction of lymphocytes activated marker CD69 following exposure to chitosan and alginate biopolymers |
spellingShingle |
Induction of lymphocytes activated marker CD69 following exposure to chitosan and alginate biopolymers Borges, Olga CD69 expression Chitosan Alginate CpG ODN Lymphocyte activation |
title_short |
Induction of lymphocytes activated marker CD69 following exposure to chitosan and alginate biopolymers |
title_full |
Induction of lymphocytes activated marker CD69 following exposure to chitosan and alginate biopolymers |
title_fullStr |
Induction of lymphocytes activated marker CD69 following exposure to chitosan and alginate biopolymers |
title_full_unstemmed |
Induction of lymphocytes activated marker CD69 following exposure to chitosan and alginate biopolymers |
title_sort |
Induction of lymphocytes activated marker CD69 following exposure to chitosan and alginate biopolymers |
author |
Borges, Olga |
author_facet |
Borges, Olga Borchard, Gerrit Sousa, Adriano de Junginger, Hans E. Cordeiro-da-Silva, Anabela |
author_role |
author |
author2 |
Borchard, Gerrit Sousa, Adriano de Junginger, Hans E. Cordeiro-da-Silva, Anabela |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Borges, Olga Borchard, Gerrit Sousa, Adriano de Junginger, Hans E. Cordeiro-da-Silva, Anabela |
dc.subject.por.fl_str_mv |
CD69 expression Chitosan Alginate CpG ODN Lymphocyte activation |
topic |
CD69 expression Chitosan Alginate CpG ODN Lymphocyte activation |
description |
CD69 is a very early cell activation antigen expressed on the surface of activated immune cells. It can appear within 1-2 h of activation and exhibits maximal expression levels between 18 and 24 h after stimulation. In this work, the expression profile of CD69 in mice splenocytes was evaluated following exposure to the biopolymers, chitosan or alginate and the immunostimulatory factors, CpG ODN 1826 or concanavalin A. We have shown that both polymers are able to upregulate expression of CD69 on B cells and CD4+ T-lymphocytes, with alginate as the least potent stimulus. Moreover, the expression of the CD69 molecule on CD8+ T-lymphocytes was observed only in splenocytes cultured with chitosan. However, activation of lymphocytes did not result in cell proliferation. On the other hand, CpG ODN proved to be a potent B cell stimulator, as evidenced by the upregulation of CD69, but had less effect on T-cells. These results, together with previous discoveries reported in scientific literature, may contribute to the clarification of the adjuvant effect, which has been attributed to chitosan and alginate formulations or to the biopolymers itself. |
publishDate |
2007 |
dc.date.none.fl_str_mv |
2007 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10316/5858 http://hdl.handle.net/10316/5858 https://doi.org/10.1016/j.ijpharm.2007.01.021 |
url |
http://hdl.handle.net/10316/5858 https://doi.org/10.1016/j.ijpharm.2007.01.021 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
International Journal of Pharmaceutics. 337:1-2 (2007) 254-264 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
aplication/PDF |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799133751182098432 |