Evaluation of the humoral and cellular immune response to different antigens of Corynebacterium pseudotuberculosis in Caninde´ goats and their potential protection against caseous lymphadenitis
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2008 |
| Outros Autores: | , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da UFBA |
| Texto Completo: | http://www.repositorio.ufba.br/ri/handle/ri/4826 |
Resumo: | P.131–141 |
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Costa, Lília Ferreira de MouraBahia, Robson CerqueiraCarminati, RenatoVale, Vera Lúcia CostaPaule, Bruno Jean AdrienPortela, Ricardo Wagner DiasFreire, Songeli MenezesNascimento, Ivana Lúcia de OliveiraSchaer, Robert EduardBarreto, Luiz Miguel SantosNascimento, Roberto José MeyerCosta, Lília Ferreira de MouraBahia, Robson CerqueiraCarminati, RenatoVale, Vera Lúcia CostaPaule, Bruno Jean AdrienPortela, Ricardo Wagner DiasFreire, Songeli MenezesNascimento, Ivana Lúcia de OliveiraSchaer, Robert EduardBarreto, Luiz Miguel SantosNascimento, Roberto José Meyer2011-12-12T18:26:43Z2011-12-12T18:26:43Z2008http://www.repositorio.ufba.br/ri/handle/ri/4826v.126P.131–141Corynebacterium pseudotuberculosis is the etiologic agent of caseous lymphadenitis, a disease that affects goats and sheep, and can cause severe economic losses. In this study, four different antigenic extracts were obtained from the attenuated strain T1, which was isolated in the state of Bahia (Brazil). Forty-four Caninde´ breed goats were divided in five groups, each receiving a different antigen solution and saline buffer as a control. The humoral response was monitored through the identification of specific IgG by indirect ELISA and Western Blotting, and the production of IFN-g was followed in order to observe the activation of cellular response. After twelve weeks of antigen inoculation, the animals were challenged with 2 105 CFU of a wild strain, also isolated in Bahia, and necropsy was performed on all animals twelve weeks afterwards. It was observed that the attenuated bacteria gave a protection of 33.3%, in addition to the weak humoral response elicited. Animals inoculated with secreted antigen associated with Freund’s incomplete adjuvant and oligodeoxynucleotide containing unmethylated CpG dinucleotides (CpG ODN) showed a strong humoral response, but this inoculation could not prevent the spread of challenge bacteria in the majority of animals. These results demonstrate the immunogenic potential of the attenuated T1 strain in the development of a vaccine against caseous lymphadenitis in goats.Submitted by Rigaud Andréa (andrearigaud16@yahoo.com.br) on 2011-12-12T18:26:43Z No. of bitstreams: 1 http___www.sciencedirect.com_science__ob=MiamiImageURL&_cid=271206&_user=686342&_pii=S0165242708002729&_check=y&_origin=&_coverDate=15-Nov-2008&view=c&wchp=dGLzVlV-zSkWA&md5=e94a2cd032181b80cdf7b090e.pdf: 785318 bytes, checksum: 7ff75d64a24dd02a7c1487f234150b67 (MD5)Made available in DSpace on 2011-12-12T18:26:43Z (GMT). No. of bitstreams: 1 http___www.sciencedirect.com_science__ob=MiamiImageURL&_cid=271206&_user=686342&_pii=S0165242708002729&_check=y&_origin=&_coverDate=15-Nov-2008&view=c&wchp=dGLzVlV-zSkWA&md5=e94a2cd032181b80cdf7b090e.pdf: 785318 bytes, checksum: 7ff75d64a24dd02a7c1487f234150b67 (MD5) Previous issue date: 2008Caseous lymphadenitisGoatsC. pseudotuberculosisCpG ODNVaccineEvaluation of the humoral and cellular immune response to different antigens of Corynebacterium pseudotuberculosis in Caninde´ goats and their potential protection against caseous lymphadenitisVeterinary Immunology and Immunopathologyinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleengreponame:Repositório Institucional da UFBAinstname:Universidade Federal da Bahia 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| dc.title.pt_BR.fl_str_mv |
Evaluation of the humoral and cellular immune response to different antigens of Corynebacterium pseudotuberculosis in Caninde´ goats and their potential protection against caseous lymphadenitis |
| dc.title.alternative.pt_BR.fl_str_mv |
Veterinary Immunology and Immunopathology |
| title |
Evaluation of the humoral and cellular immune response to different antigens of Corynebacterium pseudotuberculosis in Caninde´ goats and their potential protection against caseous lymphadenitis |
| spellingShingle |
Evaluation of the humoral and cellular immune response to different antigens of Corynebacterium pseudotuberculosis in Caninde´ goats and their potential protection against caseous lymphadenitis Costa, Lília Ferreira de Moura Caseous lymphadenitis Goats C. pseudotuberculosis CpG ODN Vaccine |
| title_short |
Evaluation of the humoral and cellular immune response to different antigens of Corynebacterium pseudotuberculosis in Caninde´ goats and their potential protection against caseous lymphadenitis |
| title_full |
Evaluation of the humoral and cellular immune response to different antigens of Corynebacterium pseudotuberculosis in Caninde´ goats and their potential protection against caseous lymphadenitis |
| title_fullStr |
Evaluation of the humoral and cellular immune response to different antigens of Corynebacterium pseudotuberculosis in Caninde´ goats and their potential protection against caseous lymphadenitis |
| title_full_unstemmed |
Evaluation of the humoral and cellular immune response to different antigens of Corynebacterium pseudotuberculosis in Caninde´ goats and their potential protection against caseous lymphadenitis |
| title_sort |
Evaluation of the humoral and cellular immune response to different antigens of Corynebacterium pseudotuberculosis in Caninde´ goats and their potential protection against caseous lymphadenitis |
| author |
Costa, Lília Ferreira de Moura |
| author_facet |
Costa, Lília Ferreira de Moura Bahia, Robson Cerqueira Carminati, Renato Vale, Vera Lúcia Costa Paule, Bruno Jean Adrien Portela, Ricardo Wagner Dias Freire, Songeli Menezes Nascimento, Ivana Lúcia de Oliveira Schaer, Robert Eduard Barreto, Luiz Miguel Santos Nascimento, Roberto José Meyer |
| author_role |
author |
| author2 |
Bahia, Robson Cerqueira Carminati, Renato Vale, Vera Lúcia Costa Paule, Bruno Jean Adrien Portela, Ricardo Wagner Dias Freire, Songeli Menezes Nascimento, Ivana Lúcia de Oliveira Schaer, Robert Eduard Barreto, Luiz Miguel Santos Nascimento, Roberto José Meyer |
| author2_role |
author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Costa, Lília Ferreira de Moura Bahia, Robson Cerqueira Carminati, Renato Vale, Vera Lúcia Costa Paule, Bruno Jean Adrien Portela, Ricardo Wagner Dias Freire, Songeli Menezes Nascimento, Ivana Lúcia de Oliveira Schaer, Robert Eduard Barreto, Luiz Miguel Santos Nascimento, Roberto José Meyer Costa, Lília Ferreira de Moura Bahia, Robson Cerqueira Carminati, Renato Vale, Vera Lúcia Costa Paule, Bruno Jean Adrien Portela, Ricardo Wagner Dias Freire, Songeli Menezes Nascimento, Ivana Lúcia de Oliveira Schaer, Robert Eduard Barreto, Luiz Miguel Santos Nascimento, Roberto José Meyer |
| dc.subject.por.fl_str_mv |
Caseous lymphadenitis Goats C. pseudotuberculosis CpG ODN Vaccine |
| topic |
Caseous lymphadenitis Goats C. pseudotuberculosis CpG ODN Vaccine |
| description |
P.131–141 |
| publishDate |
2008 |
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2008 |
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2011-12-12T18:26:43Z |
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2011-12-12T18:26:43Z |
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info:eu-repo/semantics/article |
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http://www.repositorio.ufba.br/ri/handle/ri/4826 |
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v.126 |
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eng |
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