Evaluation of the humoral and cellular immune response to different antigens of Corynebacterium pseudotuberculosis in Caninde´ goats and their potential protection against caseous lymphadenitis

Detalhes bibliográficos
Autor(a) principal: Costa, Lília Ferreira de Moura
Data de Publicação: 2008
Outros Autores: Cerqueira, Robson Bahia, Carminati, Renato, Vale, Vera Lúcia Costa, Paule, Bruno Jean Adrien, Portela, Ricardo Wagner Dias, Freire, Songeli Menezes, Nascimento, Ivana Lúcia de Oliveira, Schaer, Robert Eduard, Barreto, Luiz Miguel Santos, Nascimento, Roberto José Meyer
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFBA
Texto Completo: http://www.repositorio.ufba.br/ri/handle/ri/5246
Resumo: p.131–141
id UFBA-2_d32fbacabb4e064d639abda86fa08633
oai_identifier_str oai:repositorio.ufba.br:ri/5246
network_acronym_str UFBA-2
network_name_str Repositório Institucional da UFBA
repository_id_str 1932
spelling Costa, Lília Ferreira de MouraCerqueira, Robson BahiaCarminati, RenatoVale, Vera Lúcia CostaPaule, Bruno Jean AdrienPortela, Ricardo Wagner DiasFreire, Songeli MenezesNascimento, Ivana Lúcia de OliveiraSchaer, Robert EduardBarreto, Luiz Miguel SantosNascimento, Roberto José MeyerCosta, Lília Ferreira de MouraCerqueira, Robson BahiaCarminati, RenatoVale, Vera Lúcia CostaPaule, Bruno Jean AdrienPortela, Ricardo Wagner DiasFreire, Songeli MenezesNascimento, Ivana Lúcia de OliveiraSchaer, Robert EduardBarreto, Luiz Miguel SantosNascimento, Roberto José Meyer2012-01-25T12:14:46Z2012-01-25T12:14:46Z20080165-2427http://www.repositorio.ufba.br/ri/handle/ri/5246v. 126.p.131–141Corynebacterium pseudotuberculosis is the etiologic agent of caseous lymphadenitis, a disease that affects goats and sheep, and can cause severe economic losses. In this study, four different antigenic extracts were obtained from the attenuated strain T1, which was isolated in the state of Bahia (Brazil). Forty-four Caninde´ breed goats were divided in five groups, each receiving a different antigen solution and saline buffer as a control. The humoral response was monitored through the identification of specific IgG by indirect ELISA and Western Blotting, and the production of IFN-g was followed in order to observe the activation of cellular response. After twelve weeks of antigen inoculation, the animals were challenged with 2 105 CFU of a wild strain, also isolated in Bahia, and necropsy was performed on all animals twelve weeks afterwards. It was observed that the attenuated bacteria gave a protection of 33.3%, in addition to the weak humoral response elicited. Animals inoculated with secreted antigen associated with Freund’s incomplete adjuvant and oligodeoxynucleotide containing unmethylated CpG dinucleotides (CpG ODN) showed a strong humoral response, but this inoculation could not prevent the spread of challenge bacteria in the majority of animals. These results demonstrate the immunogenic potential of the attenuated T1 strain in the development of a vaccine against caseous lymphadenitis in goats.Submitted by Ana Valéria de Jesus Moura (anavaleria_131@hotmail.com) on 2012-01-25T12:14:46Z No. of bitstreams: 1 S0165242708002729-main.pdf: 785318 bytes, checksum: 7ff75d64a24dd02a7c1487f234150b67 (MD5)Made available in DSpace on 2012-01-25T12:14:46Z (GMT). No. of bitstreams: 1 S0165242708002729-main.pdf: 785318 bytes, checksum: 7ff75d64a24dd02a7c1487f234150b67 (MD5) Previous issue date: 2008Caseous lymphadenitisGoatsC. pseudotuberculosisCpG ODNVaccineEvaluation of the humoral and cellular immune response to different antigens of Corynebacterium pseudotuberculosis in Caninde´ goats and their potential protection against caseous lymphadenitisVeterinary Immunology and Immunopathologyinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleengreponame:Repositório Institucional da UFBAinstname:Universidade Federal da Bahia (UFBA)instacron:UFBAinfo:eu-repo/semantics/openAccessORIGINALS0165242708002729-main.pdfS0165242708002729-main.pdfapplication/pdf785318https://repositorio.ufba.br/bitstream/ri/5246/1/S0165242708002729-main.pdf7ff75d64a24dd02a7c1487f234150b67MD51LICENSElicense.txtlicense.txttext/plain1762https://repositorio.ufba.br/bitstream/ri/5246/2/license.txt1b89a9a0548218172d7c829f87a0eab9MD52TEXTS0165242708002729-main.pdf.txtS0165242708002729-main.pdf.txtExtracted texttext/plain44688https://repositorio.ufba.br/bitstream/ri/5246/3/S0165242708002729-main.pdf.txta81067c9e831bcb53eab371c24279a80MD53ri/52462022-08-08 13:17:48.453oai:repositorio.ufba.br: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Repositório InstitucionalPUBhttp://192.188.11.11:8080/oai/requestopendoar:19322022-08-08T16:17:48Repositório Institucional da UFBA - Universidade Federal da Bahia (UFBA)false
dc.title.pt_BR.fl_str_mv Evaluation of the humoral and cellular immune response to different antigens of Corynebacterium pseudotuberculosis in Caninde´ goats and their potential protection against caseous lymphadenitis
dc.title.alternative.pt_BR.fl_str_mv Veterinary Immunology and Immunopathology
title Evaluation of the humoral and cellular immune response to different antigens of Corynebacterium pseudotuberculosis in Caninde´ goats and their potential protection against caseous lymphadenitis
spellingShingle Evaluation of the humoral and cellular immune response to different antigens of Corynebacterium pseudotuberculosis in Caninde´ goats and their potential protection against caseous lymphadenitis
Costa, Lília Ferreira de Moura
Caseous lymphadenitis
Goats
C. pseudotuberculosis
CpG ODN
Vaccine
title_short Evaluation of the humoral and cellular immune response to different antigens of Corynebacterium pseudotuberculosis in Caninde´ goats and their potential protection against caseous lymphadenitis
title_full Evaluation of the humoral and cellular immune response to different antigens of Corynebacterium pseudotuberculosis in Caninde´ goats and their potential protection against caseous lymphadenitis
title_fullStr Evaluation of the humoral and cellular immune response to different antigens of Corynebacterium pseudotuberculosis in Caninde´ goats and their potential protection against caseous lymphadenitis
title_full_unstemmed Evaluation of the humoral and cellular immune response to different antigens of Corynebacterium pseudotuberculosis in Caninde´ goats and their potential protection against caseous lymphadenitis
title_sort Evaluation of the humoral and cellular immune response to different antigens of Corynebacterium pseudotuberculosis in Caninde´ goats and their potential protection against caseous lymphadenitis
author Costa, Lília Ferreira de Moura
author_facet Costa, Lília Ferreira de Moura
Cerqueira, Robson Bahia
Carminati, Renato
Vale, Vera Lúcia Costa
Paule, Bruno Jean Adrien
Portela, Ricardo Wagner Dias
Freire, Songeli Menezes
Nascimento, Ivana Lúcia de Oliveira
Schaer, Robert Eduard
Barreto, Luiz Miguel Santos
Nascimento, Roberto José Meyer
author_role author
author2 Cerqueira, Robson Bahia
Carminati, Renato
Vale, Vera Lúcia Costa
Paule, Bruno Jean Adrien
Portela, Ricardo Wagner Dias
Freire, Songeli Menezes
Nascimento, Ivana Lúcia de Oliveira
Schaer, Robert Eduard
Barreto, Luiz Miguel Santos
Nascimento, Roberto José Meyer
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Costa, Lília Ferreira de Moura
Cerqueira, Robson Bahia
Carminati, Renato
Vale, Vera Lúcia Costa
Paule, Bruno Jean Adrien
Portela, Ricardo Wagner Dias
Freire, Songeli Menezes
Nascimento, Ivana Lúcia de Oliveira
Schaer, Robert Eduard
Barreto, Luiz Miguel Santos
Nascimento, Roberto José Meyer
Costa, Lília Ferreira de Moura
Cerqueira, Robson Bahia
Carminati, Renato
Vale, Vera Lúcia Costa
Paule, Bruno Jean Adrien
Portela, Ricardo Wagner Dias
Freire, Songeli Menezes
Nascimento, Ivana Lúcia de Oliveira
Schaer, Robert Eduard
Barreto, Luiz Miguel Santos
Nascimento, Roberto José Meyer
dc.subject.por.fl_str_mv Caseous lymphadenitis
Goats
C. pseudotuberculosis
CpG ODN
Vaccine
topic Caseous lymphadenitis
Goats
C. pseudotuberculosis
CpG ODN
Vaccine
description p.131–141
publishDate 2008
dc.date.issued.fl_str_mv 2008
dc.date.accessioned.fl_str_mv 2012-01-25T12:14:46Z
dc.date.available.fl_str_mv 2012-01-25T12:14:46Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.repositorio.ufba.br/ri/handle/ri/5246
dc.identifier.issn.none.fl_str_mv 0165-2427
dc.identifier.number.pt_BR.fl_str_mv v. 126.
identifier_str_mv 0165-2427
v. 126.
url http://www.repositorio.ufba.br/ri/handle/ri/5246
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFBA
instname:Universidade Federal da Bahia (UFBA)
instacron:UFBA
instname_str Universidade Federal da Bahia (UFBA)
instacron_str UFBA
institution UFBA
reponame_str Repositório Institucional da UFBA
collection Repositório Institucional da UFBA
bitstream.url.fl_str_mv https://repositorio.ufba.br/bitstream/ri/5246/1/S0165242708002729-main.pdf
https://repositorio.ufba.br/bitstream/ri/5246/2/license.txt
https://repositorio.ufba.br/bitstream/ri/5246/3/S0165242708002729-main.pdf.txt
bitstream.checksum.fl_str_mv 7ff75d64a24dd02a7c1487f234150b67
1b89a9a0548218172d7c829f87a0eab9
a81067c9e831bcb53eab371c24279a80
bitstream.checksumAlgorithm.fl_str_mv MD5
MD5
MD5
repository.name.fl_str_mv Repositório Institucional da UFBA - Universidade Federal da Bahia (UFBA)
repository.mail.fl_str_mv
_version_ 1808459393308557312