Notch signaling in mouse blastocyst development and hatching
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.5/24102 |
Resumo: | Research Areas: Developmental Biology |
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7160 |
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Notch signaling in mouse blastocyst development and hatchingBlastocystDevelopmentHatchingNotchMouseResearch Areas: Developmental BiologyBackground: Mammalian early embryo development requires a well-orchestrated interplay of cell signaling pathways. Notch is a major regulatory pathway involved in cell-fate determination in embryonic and adult scenarios. However, the role of Notch in embryonic pre-implantation development is controversial. In particular, Notch role on blastocyst development and hatching remains elusive, and a complete picture of the transcription and expression patterns of Notch components during this time-period is not available. Results: This study provided a comprehensive view on the dynamics of individual embryo gene transcription and protein expression patterns of Notch components (receptors Notch1–4; ligands Dll1 and Dll4, Jagged1–2; and effectors Hes1–2), and their relationship with transcription of gene markers of pluripotency and differentiation (Sox2, Oct4, Klf4, Cdx2) during mouse blastocyst development and hatching. Transcription of Notch1–2, Jagged1–2 and Hes1 was highly prevalent and dynamic along stages of development, whereas transcription of Notch3–4, Dll4 and Hes2 had a low prevalence among embryos. Transcription levels of Notch1, Notch2, Jagged2 and Hes1 correlated with each other and with those of pluripotency and differentiation genes. Gene transcription was associated to protein expression, except for Jagged2, where high transcription levels in all embryos were not translated into protein. Presence of Notch signaling activity was confirmed through nuclear NICD and Hes1 detection, and downregulation of Hes1 transcription following canonical signaling blockade with DAPT. In vitro embryo culture supplementation with Jagged1 had no effect on embryo developmental kinetics. In contrast, supplementation with Jagged2 abolished Jagged1 transcription, downregulated Cdx2 transcription and inhibited blastocyst hatching. Notch signaling blockade by DAPT downregulated transcription of Sox2, and retarded embryo hatching. Conclusion: Transcription of Notch genes showed a dynamic pattern along blastocyst development and hatching. Data confirmed Notch signaling activity, and lead to the suggestion that Notch canonical signaling may be operating through Notch1, Notch3, Jagged1 and Hes1. Embryo culture supplementation with Jagged1 and Jagged2 unveiled a possible regulatory effect between Jagged1, Cdx2 and blastocyst hatching. Overall, results indicate that a deregulation in Notch signaling, either by its over or under-activation, affects blastocyst development and hatching.BMCRepositório da Universidade de LisboaBatista, Mariana R.Diniz, PatríciaTorres, Anade Moura Murta, DanielLopes-da-Costa, LuísSilva, Elisabete2022-04-18T16:51:54Z2020-06-022020-06-02T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.5/24102engBatista MR, Diniz P, Torres A, Murta D, Lopes-da-Costa L, Silva E. 2020. Notch signaling in mouse blastocyst development and hatching, BMC Developmental Biology, 20(1):9. Doi 10.1186/s12861-020-00216-21471-213X10.1186/s12861-020-00216-2info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-03-06T14:53:44Zoai:www.repository.utl.pt:10400.5/24102Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T17:08:13.415025Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Notch signaling in mouse blastocyst development and hatching |
title |
Notch signaling in mouse blastocyst development and hatching |
spellingShingle |
Notch signaling in mouse blastocyst development and hatching Batista, Mariana R. Blastocyst Development Hatching Notch Mouse |
title_short |
Notch signaling in mouse blastocyst development and hatching |
title_full |
Notch signaling in mouse blastocyst development and hatching |
title_fullStr |
Notch signaling in mouse blastocyst development and hatching |
title_full_unstemmed |
Notch signaling in mouse blastocyst development and hatching |
title_sort |
Notch signaling in mouse blastocyst development and hatching |
author |
Batista, Mariana R. |
author_facet |
Batista, Mariana R. Diniz, Patrícia Torres, Ana de Moura Murta, Daniel Lopes-da-Costa, Luís Silva, Elisabete |
author_role |
author |
author2 |
Diniz, Patrícia Torres, Ana de Moura Murta, Daniel Lopes-da-Costa, Luís Silva, Elisabete |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Repositório da Universidade de Lisboa |
dc.contributor.author.fl_str_mv |
Batista, Mariana R. Diniz, Patrícia Torres, Ana de Moura Murta, Daniel Lopes-da-Costa, Luís Silva, Elisabete |
dc.subject.por.fl_str_mv |
Blastocyst Development Hatching Notch Mouse |
topic |
Blastocyst Development Hatching Notch Mouse |
description |
Research Areas: Developmental Biology |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-06-02 2020-06-02T00:00:00Z 2022-04-18T16:51:54Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.5/24102 |
url |
http://hdl.handle.net/10400.5/24102 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Batista MR, Diniz P, Torres A, Murta D, Lopes-da-Costa L, Silva E. 2020. Notch signaling in mouse blastocyst development and hatching, BMC Developmental Biology, 20(1):9. Doi 10.1186/s12861-020-00216-2 1471-213X 10.1186/s12861-020-00216-2 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
BMC |
publisher.none.fl_str_mv |
BMC |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
|
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1799131176096497664 |