Investigation of the Molecular Mechanisms of the Eukaryotic Cytochrome-c Maturation System

Detalhes bibliográficos
Autor(a) principal: Silva, Ana V.
Data de Publicação: 2022
Outros Autores: Firmino, Maria O., Costa, Nazua L., Louro, Ricardo O., Paquete, Catarina M.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/142224
Resumo: Funding Information: Financial support was provided by European EC Horizon 2020 TIMB3 (Project 810856). Financial support was also provided by Project MOSTMICRO-ITQB with refs UIDB/04612/2020 and UIDP/04612/2020. Fundação para a Ciência e a Tecnologia (FCT) Portugal is also acknowledged for funding through project PTDC/BIA-BQM/30176/2017, and through SFRH/BD/129067/2017 to A.V.S. Funding Information: Funding: Financial support was provided by European EC Horizon2020 TIMB3 (Project 810856). Financial support was also provided by Project MOSTMICRO-ITQB with refs UIDB/04612/2020 and UIDP/04612/2020. Fundação para a Ciência e a Tecnologia (FCT) Portugal is also acknowledged for funding through project PTDC/BIA-BQM/30176/2017, and through SFRH/BD/129067/2017 to A.V.S. Publisher Copyright: © 2022 by the authors. Licensee MDPI, Basel, Switzerland.
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spelling Investigation of the Molecular Mechanisms of the Eukaryotic Cytochrome-c Maturation SystemCcHLlyasemultiheme cytochromeSmall Tetraheme CytochromeBiochemistryMolecular BiologyFunding Information: Financial support was provided by European EC Horizon 2020 TIMB3 (Project 810856). Financial support was also provided by Project MOSTMICRO-ITQB with refs UIDB/04612/2020 and UIDP/04612/2020. Fundação para a Ciência e a Tecnologia (FCT) Portugal is also acknowledged for funding through project PTDC/BIA-BQM/30176/2017, and through SFRH/BD/129067/2017 to A.V.S. Funding Information: Funding: Financial support was provided by European EC Horizon2020 TIMB3 (Project 810856). Financial support was also provided by Project MOSTMICRO-ITQB with refs UIDB/04612/2020 and UIDP/04612/2020. Fundação para a Ciência e a Tecnologia (FCT) Portugal is also acknowledged for funding through project PTDC/BIA-BQM/30176/2017, and through SFRH/BD/129067/2017 to A.V.S. Publisher Copyright: © 2022 by the authors. Licensee MDPI, Basel, Switzerland.Cytochromes-c are ubiquitous heme proteins with enormous impact at the cellular level, being key players in metabolic processes such as electron transfer chains and apoptosis. The assembly of these proteins requires maturation systems that catalyse the formation of the covalent thioether bond between two cysteine residues and the vinyl groups of the heme. System III is the maturation system present in Eukaryotes, designated CcHL or HCCS. This System requires a specific amino acid sequence in the apocytochrome to be recognized as a substrate and for heme insertion. To explore the recognition mechanisms of CcHL, the bacterial tetraheme cytochrome STC from Shewanella oneidensis MR-1, which is not a native substrate for System III, was mutated to be identified as a substrate. The results obtained show that it is possible to convert a bacterial cytochrome as a substrate by CcHL, but the presence of the recognition sequence is not the only factor that induces the maturation of a holocytochrome by System III. The location of this sequence in the polypeptide also plays a role in the maturation of the c-type cytochrome. Furthermore, CcHL appears to be able to catalyse the binding of only one heme per polypeptide chain, being unable to assemble multiheme cytochromes c, in contrast with bacterial maturation systems.Instituto de Tecnologia Química e Biológica António Xavier (ITQB)RUNSilva, Ana V.Firmino, Maria O.Costa, Nazua L.Louro, Ricardo O.Paquete, Catarina M.2022-07-20T22:31:37Z2022-042022-04-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/142224eng2218-273XPURE: 45490388https://doi.org/10.3390/biom12040549info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T05:19:44Zoai:run.unl.pt:10362/142224Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:50:12.617422Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Investigation of the Molecular Mechanisms of the Eukaryotic Cytochrome-c Maturation System
title Investigation of the Molecular Mechanisms of the Eukaryotic Cytochrome-c Maturation System
spellingShingle Investigation of the Molecular Mechanisms of the Eukaryotic Cytochrome-c Maturation System
Silva, Ana V.
CcHL
lyase
multiheme cytochrome
Small Tetraheme Cytochrome
Biochemistry
Molecular Biology
title_short Investigation of the Molecular Mechanisms of the Eukaryotic Cytochrome-c Maturation System
title_full Investigation of the Molecular Mechanisms of the Eukaryotic Cytochrome-c Maturation System
title_fullStr Investigation of the Molecular Mechanisms of the Eukaryotic Cytochrome-c Maturation System
title_full_unstemmed Investigation of the Molecular Mechanisms of the Eukaryotic Cytochrome-c Maturation System
title_sort Investigation of the Molecular Mechanisms of the Eukaryotic Cytochrome-c Maturation System
author Silva, Ana V.
author_facet Silva, Ana V.
Firmino, Maria O.
Costa, Nazua L.
Louro, Ricardo O.
Paquete, Catarina M.
author_role author
author2 Firmino, Maria O.
Costa, Nazua L.
Louro, Ricardo O.
Paquete, Catarina M.
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Instituto de Tecnologia Química e Biológica António Xavier (ITQB)
RUN
dc.contributor.author.fl_str_mv Silva, Ana V.
Firmino, Maria O.
Costa, Nazua L.
Louro, Ricardo O.
Paquete, Catarina M.
dc.subject.por.fl_str_mv CcHL
lyase
multiheme cytochrome
Small Tetraheme Cytochrome
Biochemistry
Molecular Biology
topic CcHL
lyase
multiheme cytochrome
Small Tetraheme Cytochrome
Biochemistry
Molecular Biology
description Funding Information: Financial support was provided by European EC Horizon 2020 TIMB3 (Project 810856). Financial support was also provided by Project MOSTMICRO-ITQB with refs UIDB/04612/2020 and UIDP/04612/2020. Fundação para a Ciência e a Tecnologia (FCT) Portugal is also acknowledged for funding through project PTDC/BIA-BQM/30176/2017, and through SFRH/BD/129067/2017 to A.V.S. Funding Information: Funding: Financial support was provided by European EC Horizon2020 TIMB3 (Project 810856). Financial support was also provided by Project MOSTMICRO-ITQB with refs UIDB/04612/2020 and UIDP/04612/2020. Fundação para a Ciência e a Tecnologia (FCT) Portugal is also acknowledged for funding through project PTDC/BIA-BQM/30176/2017, and through SFRH/BD/129067/2017 to A.V.S. Publisher Copyright: © 2022 by the authors. Licensee MDPI, Basel, Switzerland.
publishDate 2022
dc.date.none.fl_str_mv 2022-07-20T22:31:37Z
2022-04
2022-04-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/142224
url http://hdl.handle.net/10362/142224
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 2218-273X
PURE: 45490388
https://doi.org/10.3390/biom12040549
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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