Genomic Epidemiology and Phenotyping Reveal on-Farm Persistence and Cold Adaptation of Raw Milk Outbreak-Associated Yersinia pseudotuberculosis

Detalhes bibliográficos
Autor(a) principal: Castro, Hanna
Data de Publicação: 2019
Outros Autores: Jaakkonen, Anniina, Hakakorpi, Anna, Hakkinen, Marjaana, Isidro, Joana, Korkeala, Hannu, Lindström, Miia, Hallanvuo, Saija
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.18/6628
Resumo: Free PMC article: https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/31156582/
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spelling Genomic Epidemiology and Phenotyping Reveal on-Farm Persistence and Cold Adaptation of Raw Milk Outbreak-Associated Yersinia pseudotuberculosisBiofilmComparative GenomicsFood SafetyGrowth ModelingPhylogenomicsRaw MilkStress ToleranceWhole Genome SequencingInfecções GastrointestinaisFree PMC article: https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/31156582/Packaged raw milk contaminated with Yersinia pseudotuberculosis mediated a large yersiniosis outbreak in southern Finland in 2014. The outbreak was traced back to a single dairy farm in southern Finland. Here we explore risk factors leading to the outbreak through epidemiologic investigation of the outbreak farm and through genomic and phenotypic characterization of the farm's outbreak and non-outbreak associated Y. pseudotuberculosis strains. We show that the outbreak strain persisted on the farm throughout the 7-month study, whereas the non-outbreak strains occurred sporadically. Phylogenomic analysis illustrated that the outbreak strain was related to previously published genomes of wild animal isolates from Finland, implying that wild animals were a potential source of the outbreak strain to the farm. We observed allelic differences between the farm's outbreak and non-outbreak strains in several genes associated with virulence, stress response and biofilm formation, and found that the outbreak strain formed biofilm in vitro and maintained better growth fitness during cold stress than the non-outbreak strains. Finally, we demonstrate the rapid growth of the outbreak strain in packaged raw milk during refrigerated storage. This study provides insight of the risk factors leading to the Y. pseudotuberculosis outbreak, highlights the importance of pest control to avoid the spread of pathogens from wild to domestic animals, and demonstrates that the cold chain is insufficient as the sole risk management strategy to control Y. pseudotuberculosis risk associated with raw drinking milk.This research was supported by the Finnish Ministry of Agriculture and Forestry grant Dnro: 1395/311/214, the Walter Ehrström Foundation, and the University of Helsinki Doctoral Program in Food Chain and Health. The sequencing of the strains and the salary of AJ and JI were supported by the INNUENDO project (https://sites.google.com/site/theinnuendoproject/) which received funding from the European Food Safety Authority, grant agreement GP/EFSA/AFSCO/2015/01/CT2 (“New approaches in identifying and characterizing microbial and chemical hazards”).Frontiers MediaRepositório Científico do Instituto Nacional de SaúdeCastro, HannaJaakkonen, AnniinaHakakorpi, AnnaHakkinen, MarjaanaIsidro, JoanaKorkeala, HannuLindström, MiiaHallanvuo, Saija2020-05-09T11:35:10Z2019-05-142019-05-14T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.18/6628engFront Microbiol. 2019 May 14;10:1049. doi: 10.3389/fmicb.2019.01049. eCollection 20191664-302X10.3389/fmicb.2019.01049info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-20T15:41:46Zoai:repositorio.insa.pt:10400.18/6628Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:41:42.928623Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Genomic Epidemiology and Phenotyping Reveal on-Farm Persistence and Cold Adaptation of Raw Milk Outbreak-Associated Yersinia pseudotuberculosis
title Genomic Epidemiology and Phenotyping Reveal on-Farm Persistence and Cold Adaptation of Raw Milk Outbreak-Associated Yersinia pseudotuberculosis
spellingShingle Genomic Epidemiology and Phenotyping Reveal on-Farm Persistence and Cold Adaptation of Raw Milk Outbreak-Associated Yersinia pseudotuberculosis
Castro, Hanna
Biofilm
Comparative Genomics
Food Safety
Growth Modeling
Phylogenomics
Raw Milk
Stress Tolerance
Whole Genome Sequencing
Infecções Gastrointestinais
title_short Genomic Epidemiology and Phenotyping Reveal on-Farm Persistence and Cold Adaptation of Raw Milk Outbreak-Associated Yersinia pseudotuberculosis
title_full Genomic Epidemiology and Phenotyping Reveal on-Farm Persistence and Cold Adaptation of Raw Milk Outbreak-Associated Yersinia pseudotuberculosis
title_fullStr Genomic Epidemiology and Phenotyping Reveal on-Farm Persistence and Cold Adaptation of Raw Milk Outbreak-Associated Yersinia pseudotuberculosis
title_full_unstemmed Genomic Epidemiology and Phenotyping Reveal on-Farm Persistence and Cold Adaptation of Raw Milk Outbreak-Associated Yersinia pseudotuberculosis
title_sort Genomic Epidemiology and Phenotyping Reveal on-Farm Persistence and Cold Adaptation of Raw Milk Outbreak-Associated Yersinia pseudotuberculosis
author Castro, Hanna
author_facet Castro, Hanna
Jaakkonen, Anniina
Hakakorpi, Anna
Hakkinen, Marjaana
Isidro, Joana
Korkeala, Hannu
Lindström, Miia
Hallanvuo, Saija
author_role author
author2 Jaakkonen, Anniina
Hakakorpi, Anna
Hakkinen, Marjaana
Isidro, Joana
Korkeala, Hannu
Lindström, Miia
Hallanvuo, Saija
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Repositório Científico do Instituto Nacional de Saúde
dc.contributor.author.fl_str_mv Castro, Hanna
Jaakkonen, Anniina
Hakakorpi, Anna
Hakkinen, Marjaana
Isidro, Joana
Korkeala, Hannu
Lindström, Miia
Hallanvuo, Saija
dc.subject.por.fl_str_mv Biofilm
Comparative Genomics
Food Safety
Growth Modeling
Phylogenomics
Raw Milk
Stress Tolerance
Whole Genome Sequencing
Infecções Gastrointestinais
topic Biofilm
Comparative Genomics
Food Safety
Growth Modeling
Phylogenomics
Raw Milk
Stress Tolerance
Whole Genome Sequencing
Infecções Gastrointestinais
description Free PMC article: https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/31156582/
publishDate 2019
dc.date.none.fl_str_mv 2019-05-14
2019-05-14T00:00:00Z
2020-05-09T11:35:10Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.18/6628
url http://hdl.handle.net/10400.18/6628
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Front Microbiol. 2019 May 14;10:1049. doi: 10.3389/fmicb.2019.01049. eCollection 2019
1664-302X
10.3389/fmicb.2019.01049
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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