Energy Dispersive X-ray Fluorescence Analysis Imaging—Development and Applications
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Tipo de documento: | Dissertação |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10362/135615 |
Resumo: | The Energy Dispersive X-ray Fluorescence (EDXRF) analysis technique presents unique features for elemental content evaluation of varied samples, such as its non-destructive nature, high sensitivity, or reduced costs. Many EDXRF studies have been focused on the determination of elemental concentration variations between normal and tumour tissues, to compare and correlate findings with factors such as the type and the stage of tumours, or the patients’ age and sex. Mapping elemental distributions may also provide insightful information on these types of samples, namely the identification of regions of elemental accumulation or depletion, and the demarcation of tumour margins, thus contributing to the unbiased differentiation between normal and abnormal tissues. The main goals of this work are to quantitatively determine the elemental content in paired samples of normal and tumour human tissues, and to map the corresponding elemental distributions. An improved methodology for the quantitative analysis of biological tissues is introduced, to surpass existing limitations (e.g., reduced number of tissue samples) that have lead to low-accuracy quantifications and statistically irrelevant conclusions. Moreover, a Full-field Energy Dispersive X-ray Fluorescence (FF-XRF) imaging spectrometer based on the 2D Thick-COBRA (2D-THCOBRA) micropattern gas detector is presented as a novel, cost-effective tool of simple instrumentation, for detection and mapping of elements in biological tissues. Different sample sets of normal and tumour human tissues were analysed, and it was possible to quantify concentration variations of light (e.g., K) and heavy elements (e.g., Fe and Zn). However, the contribution of these results to cancer studies is minimal due to the reduced number of analysed samples. To overcome this limitation, mirror samples of "fresh" and paraffin embedded tissues were analysed, and it was verified that the embedding process alters the elemental content of tissues. As such, the monitoring of the elemental content of tissues throughout the process is suggested. The FF-XRF imaging spectrometer based on the 2D-THCOBRA detector was used to map paired samples of normal and tumour human tissues. Elements present in small amounts (a few μg/g) were detected and the corresponding distributions were mapped. Overall, the detected elements are evenly distributed on the samples’ surface, with the exception of Ca in the lung tumour tissue. Throughout the analysed sample, calcium deposits, a possible malignancy indicator, were identified. The imaging spectrometer was also applied in the study of Zebrafish exposed to Mn, Se, and Pb. Heavy metal accumulation in the intestinal region was identified in all the analysed fish. Despite the suitability of the system for these studies, the need to improve detection efficiency remains. As such, the iFluX imaging spectrometer, with a large area X-ray panel based on a redesigned 2D-THCOBRA structure, is introduced. |
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Energy Dispersive X-ray Fluorescence Analysis Imaging—Development and ApplicationsEDXRFMicro-XRFFF-XRFMPGD2D-THCOBRAelemental quantitative analysisDomínio/Área Científica::Engenharia e Tecnologia::Outras Engenharias e TecnologiasThe Energy Dispersive X-ray Fluorescence (EDXRF) analysis technique presents unique features for elemental content evaluation of varied samples, such as its non-destructive nature, high sensitivity, or reduced costs. Many EDXRF studies have been focused on the determination of elemental concentration variations between normal and tumour tissues, to compare and correlate findings with factors such as the type and the stage of tumours, or the patients’ age and sex. Mapping elemental distributions may also provide insightful information on these types of samples, namely the identification of regions of elemental accumulation or depletion, and the demarcation of tumour margins, thus contributing to the unbiased differentiation between normal and abnormal tissues. The main goals of this work are to quantitatively determine the elemental content in paired samples of normal and tumour human tissues, and to map the corresponding elemental distributions. An improved methodology for the quantitative analysis of biological tissues is introduced, to surpass existing limitations (e.g., reduced number of tissue samples) that have lead to low-accuracy quantifications and statistically irrelevant conclusions. Moreover, a Full-field Energy Dispersive X-ray Fluorescence (FF-XRF) imaging spectrometer based on the 2D Thick-COBRA (2D-THCOBRA) micropattern gas detector is presented as a novel, cost-effective tool of simple instrumentation, for detection and mapping of elements in biological tissues. Different sample sets of normal and tumour human tissues were analysed, and it was possible to quantify concentration variations of light (e.g., K) and heavy elements (e.g., Fe and Zn). However, the contribution of these results to cancer studies is minimal due to the reduced number of analysed samples. To overcome this limitation, mirror samples of "fresh" and paraffin embedded tissues were analysed, and it was verified that the embedding process alters the elemental content of tissues. As such, the monitoring of the elemental content of tissues throughout the process is suggested. The FF-XRF imaging spectrometer based on the 2D-THCOBRA detector was used to map paired samples of normal and tumour human tissues. Elements present in small amounts (a few μg/g) were detected and the corresponding distributions were mapped. Overall, the detected elements are evenly distributed on the samples’ surface, with the exception of Ca in the lung tumour tissue. Throughout the analysed sample, calcium deposits, a possible malignancy indicator, were identified. The imaging spectrometer was also applied in the study of Zebrafish exposed to Mn, Se, and Pb. Heavy metal accumulation in the intestinal region was identified in all the analysed fish. Despite the suitability of the system for these studies, the need to improve detection efficiency remains. As such, the iFluX imaging spectrometer, with a large area X-ray panel based on a redesigned 2D-THCOBRA structure, is introduced.As técnicas de análise por Fluorescência de Raios-X Dispersiva em Energia (EDXRF) apresentam características únicas para a avaliação do conteúdo elemental de diversas amostras, tais como o seu carácter não destrutivo, elevada sensibilidade ou custo reduzido. Muitos estudos têm-se focado na determinação de variações da concentração elemental entre tecidos normais e tumorais, para comparar e correlacionar resultados com diversos factores, como o tipo e estádio de tumores ou a idade e sexo dos doentes. O mapeamento da distribuição elemental poderá também providenciar informações importantes sobre este tipo de amostras, que contribuam para a diferenciação entre tecido com e sem patologia, através da identificação de regiões de acumulação ou depleção de elementos e da demarcação de margens tumorais. Os principais objectivos deste trabalho são a determinação da concentração elemental em amostras pareadas de tecidos com e sem patologia tumoral, e o mapeamento da distribuição elemental correspondente. Para tal, é introduzida uma metodologia melhorada para a quantificação elemental em tecidos biológicos, que permite ultrapassar as limitações (e.g., o número reduzido de amostras estudadas) que têm levado à obtenção de resultados pouco precisos e estatisticamente irrelevantes. Apresenta-se também um sistema de imagem full-field EDXRF (FF-XRF) baseado no detector gasoso micro-estruturado 2D Thick-COBRA (2D-THCOBRA), como uma alternativa inovadora, de instrumentação simples e custo reduzido, para detecção e mapeamento elemental em amostras biológicas. Foram analisados diferentes conjuntos de amostras de tecidos com e sem patologia tumoral, tendo sido possível quantificar a variação de elementos leves (e.g., K) e pesados (e.g., Fe e Zn). No entanto, a contribuição destes resultados para estudos de patologias tumorais é mínima devido ao número reduzido de amostras. De modo a ultrapassar esta limitação, foram analisadas amostras espelhadas de tecidos "frescos" e preservados em parafina. Verificou-se que este processo de preservação altera o contéudo elemental dos tecidos, propondo-se a sua monitorização em cada estágio. O sistema de imagem FF-XRF baseado no detector 2D-THCOBRA foi utilizado no mapeamento de amostras pareadas de tecido normal e tumoral. Foram detectados elementos presentes em quantidades reduzidas (alguns μg/g) e a sua distribuição foi mapeada. Verificou-se a distribuição homogénea de todos os elementos, à excepção de Ca em tecido tumoral do pulmão; e foram identificados aglomerados de cálcio que poderão ser uma característica de tumores malignos. O sistema FF-XRF foi também aplicado no mapeamento de peixes Zebra expostos a metais pesados, tendo sido verificada a acumulação de Mn, Se e Pb na zona intestinal dos peixes analisados. Apesar da adequação do sistema para este tipo de aplicações, permanece a necessidade de aumentar a eficiência de detecção. Neste sentido, é introduzido o sistema de imagem full-field iFluX, com um painel detector de raios-X de grande área baseado numa estrutura 2D-THCOBRA redesenhada.Santos, JoséMoreno, AldaSilva, AnaRUNCarvalho, Patrícia Miguel da Silva2022-04-01T08:17:49Z2022-022022-02-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfhttp://hdl.handle.net/10362/135615enginfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T05:13:59Zoai:run.unl.pt:10362/135615Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:48:28.195906Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Energy Dispersive X-ray Fluorescence Analysis Imaging—Development and Applications |
title |
Energy Dispersive X-ray Fluorescence Analysis Imaging—Development and Applications |
spellingShingle |
Energy Dispersive X-ray Fluorescence Analysis Imaging—Development and Applications Carvalho, Patrícia Miguel da Silva EDXRF Micro-XRF FF-XRF MPGD 2D-THCOBRA elemental quantitative analysis Domínio/Área Científica::Engenharia e Tecnologia::Outras Engenharias e Tecnologias |
title_short |
Energy Dispersive X-ray Fluorescence Analysis Imaging—Development and Applications |
title_full |
Energy Dispersive X-ray Fluorescence Analysis Imaging—Development and Applications |
title_fullStr |
Energy Dispersive X-ray Fluorescence Analysis Imaging—Development and Applications |
title_full_unstemmed |
Energy Dispersive X-ray Fluorescence Analysis Imaging—Development and Applications |
title_sort |
Energy Dispersive X-ray Fluorescence Analysis Imaging—Development and Applications |
author |
Carvalho, Patrícia Miguel da Silva |
author_facet |
Carvalho, Patrícia Miguel da Silva |
author_role |
author |
dc.contributor.none.fl_str_mv |
Santos, José Moreno, Alda Silva, Ana RUN |
dc.contributor.author.fl_str_mv |
Carvalho, Patrícia Miguel da Silva |
dc.subject.por.fl_str_mv |
EDXRF Micro-XRF FF-XRF MPGD 2D-THCOBRA elemental quantitative analysis Domínio/Área Científica::Engenharia e Tecnologia::Outras Engenharias e Tecnologias |
topic |
EDXRF Micro-XRF FF-XRF MPGD 2D-THCOBRA elemental quantitative analysis Domínio/Área Científica::Engenharia e Tecnologia::Outras Engenharias e Tecnologias |
description |
The Energy Dispersive X-ray Fluorescence (EDXRF) analysis technique presents unique features for elemental content evaluation of varied samples, such as its non-destructive nature, high sensitivity, or reduced costs. Many EDXRF studies have been focused on the determination of elemental concentration variations between normal and tumour tissues, to compare and correlate findings with factors such as the type and the stage of tumours, or the patients’ age and sex. Mapping elemental distributions may also provide insightful information on these types of samples, namely the identification of regions of elemental accumulation or depletion, and the demarcation of tumour margins, thus contributing to the unbiased differentiation between normal and abnormal tissues. The main goals of this work are to quantitatively determine the elemental content in paired samples of normal and tumour human tissues, and to map the corresponding elemental distributions. An improved methodology for the quantitative analysis of biological tissues is introduced, to surpass existing limitations (e.g., reduced number of tissue samples) that have lead to low-accuracy quantifications and statistically irrelevant conclusions. Moreover, a Full-field Energy Dispersive X-ray Fluorescence (FF-XRF) imaging spectrometer based on the 2D Thick-COBRA (2D-THCOBRA) micropattern gas detector is presented as a novel, cost-effective tool of simple instrumentation, for detection and mapping of elements in biological tissues. Different sample sets of normal and tumour human tissues were analysed, and it was possible to quantify concentration variations of light (e.g., K) and heavy elements (e.g., Fe and Zn). However, the contribution of these results to cancer studies is minimal due to the reduced number of analysed samples. To overcome this limitation, mirror samples of "fresh" and paraffin embedded tissues were analysed, and it was verified that the embedding process alters the elemental content of tissues. As such, the monitoring of the elemental content of tissues throughout the process is suggested. The FF-XRF imaging spectrometer based on the 2D-THCOBRA detector was used to map paired samples of normal and tumour human tissues. Elements present in small amounts (a few μg/g) were detected and the corresponding distributions were mapped. Overall, the detected elements are evenly distributed on the samples’ surface, with the exception of Ca in the lung tumour tissue. Throughout the analysed sample, calcium deposits, a possible malignancy indicator, were identified. The imaging spectrometer was also applied in the study of Zebrafish exposed to Mn, Se, and Pb. Heavy metal accumulation in the intestinal region was identified in all the analysed fish. Despite the suitability of the system for these studies, the need to improve detection efficiency remains. As such, the iFluX imaging spectrometer, with a large area X-ray panel based on a redesigned 2D-THCOBRA structure, is introduced. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-04-01T08:17:49Z 2022-02 2022-02-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10362/135615 |
url |
http://hdl.handle.net/10362/135615 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799138086154665984 |