Sewage sludge does not induce genotoxicity and carcinogenesis
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/S1415-47572012005000054 http://hdl.handle.net/11449/12835 |
Resumo: | Through a series of experiments, the genotoxic/mutagenic and carcinogenic potential of sewage sludge was assessed. Male Wistar rats were randomly assigned to four groups: Group 1 - negative control; Group 2 - liver carcinogenesis initiated by diethylnitrosamine (DEN; 200 mg/kg i.p.); Group 3 and G4- liver carcinogenesis initiated by DEN and fed 10,000 ppm or 50,000 ppm of sewage sludge. The animals were submitted to a 70% partial hepatectomy at the 3rd week. Livers were processed for routine histological analysis and immunohistochemistry, in order to detect glutathione S-transferase positive altered hepatocyte foci (GST-P+ AHF). Peripheral blood samples for the comet assay were obtained from the periorbital plexus immediately prior to sacrificing. Polychromatic erythrocytes (PCEs) were analyzed in femoral bone-marrow smears, and the frequencies of those micronucleated (MNPCEs) registered. There was no sewage-sludge-induced increase in frequency of either DNA damage in peripheral blood leucocytes, or MNPCEs in the femoral bone marrow. Also, there was no increase in the levels of DNA damage, in the frequency of MNPCEs, and in the development of GST-P AHF when compared with the respective control group. |
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Sewage sludge does not induce genotoxicity and carcinogenesiscomet assaymicronucleussewage genotoxicityThrough a series of experiments, the genotoxic/mutagenic and carcinogenic potential of sewage sludge was assessed. Male Wistar rats were randomly assigned to four groups: Group 1 - negative control; Group 2 - liver carcinogenesis initiated by diethylnitrosamine (DEN; 200 mg/kg i.p.); Group 3 and G4- liver carcinogenesis initiated by DEN and fed 10,000 ppm or 50,000 ppm of sewage sludge. The animals were submitted to a 70% partial hepatectomy at the 3rd week. Livers were processed for routine histological analysis and immunohistochemistry, in order to detect glutathione S-transferase positive altered hepatocyte foci (GST-P+ AHF). Peripheral blood samples for the comet assay were obtained from the periorbital plexus immediately prior to sacrificing. Polychromatic erythrocytes (PCEs) were analyzed in femoral bone-marrow smears, and the frequencies of those micronucleated (MNPCEs) registered. There was no sewage-sludge-induced increase in frequency of either DNA damage in peripheral blood leucocytes, or MNPCEs in the femoral bone marrow. Also, there was no increase in the levels of DNA damage, in the frequency of MNPCEs, and in the development of GST-P AHF when compared with the respective control group.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Centro de Avaliacao do Impacto Ambiental sobre a Saúde Humana (TOXICAN)Universidade Estadual Paulista Faculdade de Medicina Departamento de Patologia, Núcleo de Avaliação do Impacto Ambiental sobre a Saúde HumanaUniversidade de São Paulo Faculdade de Medicina Laboratório de Poluição do ArUniversidade Estadual Paulista 'Júlio Mesquita Filho' Instituto de Biociências Departamento de MorfologiaUniversidade de São Paulo Faculdade de Medicina Veterinária e Ciência Animal Departamento de PatologiaUniversidade Estadual Paulista Faculdade de Medicina Departamento de Patologia, Núcleo de Avaliação do Impacto Ambiental sobre a Saúde HumanaUniversidade Estadual Paulista 'Júlio Mesquita Filho' Instituto de Biociências Departamento de MorfologiaSociedade Brasileira de GenéticaUniversidade Estadual Paulista (Unesp)Universidade de São Paulo (USP)Silva, Paula Regina Pereira [UNESP]Barbisan, Luis Fernando [UNESP]Dagli, Maria Lúcia ZaidanSaldiva, Paulo Hilário Nascimento2014-05-20T13:37:09Z2014-05-20T13:37:09Z2012-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article657-663application/pdfhttp://dx.doi.org/10.1590/S1415-47572012005000054Genetics and Molecular Biology. Sociedade Brasileira de Genética, v. 35, n. 3, p. 657-663, 2012.1415-4757http://hdl.handle.net/11449/1283510.1590/S1415-47572012005000054S1415-47572012000400017WOS:000307726400017S1415-47572012000400017.pdf3278528112652257SciELOreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengGenetics and Molecular Biology1.4930,638info:eu-repo/semantics/openAccess2024-09-03T13:14:31Zoai:repositorio.unesp.br:11449/12835Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-03T13:14:31Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Sewage sludge does not induce genotoxicity and carcinogenesis |
title |
Sewage sludge does not induce genotoxicity and carcinogenesis |
spellingShingle |
Sewage sludge does not induce genotoxicity and carcinogenesis Silva, Paula Regina Pereira [UNESP] comet assay micronucleus sewage genotoxicity |
title_short |
Sewage sludge does not induce genotoxicity and carcinogenesis |
title_full |
Sewage sludge does not induce genotoxicity and carcinogenesis |
title_fullStr |
Sewage sludge does not induce genotoxicity and carcinogenesis |
title_full_unstemmed |
Sewage sludge does not induce genotoxicity and carcinogenesis |
title_sort |
Sewage sludge does not induce genotoxicity and carcinogenesis |
author |
Silva, Paula Regina Pereira [UNESP] |
author_facet |
Silva, Paula Regina Pereira [UNESP] Barbisan, Luis Fernando [UNESP] Dagli, Maria Lúcia Zaidan Saldiva, Paulo Hilário Nascimento |
author_role |
author |
author2 |
Barbisan, Luis Fernando [UNESP] Dagli, Maria Lúcia Zaidan Saldiva, Paulo Hilário Nascimento |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade de São Paulo (USP) |
dc.contributor.author.fl_str_mv |
Silva, Paula Regina Pereira [UNESP] Barbisan, Luis Fernando [UNESP] Dagli, Maria Lúcia Zaidan Saldiva, Paulo Hilário Nascimento |
dc.subject.por.fl_str_mv |
comet assay micronucleus sewage genotoxicity |
topic |
comet assay micronucleus sewage genotoxicity |
description |
Through a series of experiments, the genotoxic/mutagenic and carcinogenic potential of sewage sludge was assessed. Male Wistar rats were randomly assigned to four groups: Group 1 - negative control; Group 2 - liver carcinogenesis initiated by diethylnitrosamine (DEN; 200 mg/kg i.p.); Group 3 and G4- liver carcinogenesis initiated by DEN and fed 10,000 ppm or 50,000 ppm of sewage sludge. The animals were submitted to a 70% partial hepatectomy at the 3rd week. Livers were processed for routine histological analysis and immunohistochemistry, in order to detect glutathione S-transferase positive altered hepatocyte foci (GST-P+ AHF). Peripheral blood samples for the comet assay were obtained from the periorbital plexus immediately prior to sacrificing. Polychromatic erythrocytes (PCEs) were analyzed in femoral bone-marrow smears, and the frequencies of those micronucleated (MNPCEs) registered. There was no sewage-sludge-induced increase in frequency of either DNA damage in peripheral blood leucocytes, or MNPCEs in the femoral bone marrow. Also, there was no increase in the levels of DNA damage, in the frequency of MNPCEs, and in the development of GST-P AHF when compared with the respective control group. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-01-01 2014-05-20T13:37:09Z 2014-05-20T13:37:09Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/S1415-47572012005000054 Genetics and Molecular Biology. Sociedade Brasileira de Genética, v. 35, n. 3, p. 657-663, 2012. 1415-4757 http://hdl.handle.net/11449/12835 10.1590/S1415-47572012005000054 S1415-47572012000400017 WOS:000307726400017 S1415-47572012000400017.pdf 3278528112652257 |
url |
http://dx.doi.org/10.1590/S1415-47572012005000054 http://hdl.handle.net/11449/12835 |
identifier_str_mv |
Genetics and Molecular Biology. Sociedade Brasileira de Genética, v. 35, n. 3, p. 657-663, 2012. 1415-4757 10.1590/S1415-47572012005000054 S1415-47572012000400017 WOS:000307726400017 S1415-47572012000400017.pdf 3278528112652257 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Genetics and Molecular Biology 1.493 0,638 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
657-663 application/pdf |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Genética |
publisher.none.fl_str_mv |
Sociedade Brasileira de Genética |
dc.source.none.fl_str_mv |
SciELO reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
repositoriounesp@unesp.br |
_version_ |
1810021362725552128 |