Sewage sludge does not induce genotoxicity and carcinogenesis

Detalhes bibliográficos
Autor(a) principal: Silva, Paula Regina Pereira [UNESP]
Data de Publicação: 2012
Outros Autores: Barbisan, Luis Fernando [UNESP], Dagli, Maria Lúcia Zaidan, Saldiva, Paulo Hilário Nascimento
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1590/S1415-47572012005000054
http://hdl.handle.net/11449/12835
Resumo: Through a series of experiments, the genotoxic/mutagenic and carcinogenic potential of sewage sludge was assessed. Male Wistar rats were randomly assigned to four groups: Group 1 - negative control; Group 2 - liver carcinogenesis initiated by diethylnitrosamine (DEN; 200 mg/kg i.p.); Group 3 and G4- liver carcinogenesis initiated by DEN and fed 10,000 ppm or 50,000 ppm of sewage sludge. The animals were submitted to a 70% partial hepatectomy at the 3rd week. Livers were processed for routine histological analysis and immunohistochemistry, in order to detect glutathione S-transferase positive altered hepatocyte foci (GST-P+ AHF). Peripheral blood samples for the comet assay were obtained from the periorbital plexus immediately prior to sacrificing. Polychromatic erythrocytes (PCEs) were analyzed in femoral bone-marrow smears, and the frequencies of those micronucleated (MNPCEs) registered. There was no sewage-sludge-induced increase in frequency of either DNA damage in peripheral blood leucocytes, or MNPCEs in the femoral bone marrow. Also, there was no increase in the levels of DNA damage, in the frequency of MNPCEs, and in the development of GST-P AHF when compared with the respective control group.
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spelling Sewage sludge does not induce genotoxicity and carcinogenesiscomet assaymicronucleussewage genotoxicityThrough a series of experiments, the genotoxic/mutagenic and carcinogenic potential of sewage sludge was assessed. Male Wistar rats were randomly assigned to four groups: Group 1 - negative control; Group 2 - liver carcinogenesis initiated by diethylnitrosamine (DEN; 200 mg/kg i.p.); Group 3 and G4- liver carcinogenesis initiated by DEN and fed 10,000 ppm or 50,000 ppm of sewage sludge. The animals were submitted to a 70% partial hepatectomy at the 3rd week. Livers were processed for routine histological analysis and immunohistochemistry, in order to detect glutathione S-transferase positive altered hepatocyte foci (GST-P+ AHF). Peripheral blood samples for the comet assay were obtained from the periorbital plexus immediately prior to sacrificing. Polychromatic erythrocytes (PCEs) were analyzed in femoral bone-marrow smears, and the frequencies of those micronucleated (MNPCEs) registered. There was no sewage-sludge-induced increase in frequency of either DNA damage in peripheral blood leucocytes, or MNPCEs in the femoral bone marrow. Also, there was no increase in the levels of DNA damage, in the frequency of MNPCEs, and in the development of GST-P AHF when compared with the respective control group.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Centro de Avaliacao do Impacto Ambiental sobre a Saúde Humana (TOXICAN)Universidade Estadual Paulista Faculdade de Medicina Departamento de Patologia, Núcleo de Avaliação do Impacto Ambiental sobre a Saúde HumanaUniversidade de São Paulo Faculdade de Medicina Laboratório de Poluição do ArUniversidade Estadual Paulista 'Júlio Mesquita Filho' Instituto de Biociências Departamento de MorfologiaUniversidade de São Paulo Faculdade de Medicina Veterinária e Ciência Animal Departamento de PatologiaUniversidade Estadual Paulista Faculdade de Medicina Departamento de Patologia, Núcleo de Avaliação do Impacto Ambiental sobre a Saúde HumanaUniversidade Estadual Paulista 'Júlio Mesquita Filho' Instituto de Biociências Departamento de MorfologiaSociedade Brasileira de GenéticaUniversidade Estadual Paulista (Unesp)Universidade de São Paulo (USP)Silva, Paula Regina Pereira [UNESP]Barbisan, Luis Fernando [UNESP]Dagli, Maria Lúcia ZaidanSaldiva, Paulo Hilário Nascimento2014-05-20T13:37:09Z2014-05-20T13:37:09Z2012-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article657-663application/pdfhttp://dx.doi.org/10.1590/S1415-47572012005000054Genetics and Molecular Biology. Sociedade Brasileira de Genética, v. 35, n. 3, p. 657-663, 2012.1415-4757http://hdl.handle.net/11449/1283510.1590/S1415-47572012005000054S1415-47572012000400017WOS:000307726400017S1415-47572012000400017.pdf3278528112652257SciELOreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengGenetics and Molecular Biology1.4930,638info:eu-repo/semantics/openAccess2024-09-03T13:14:31Zoai:repositorio.unesp.br:11449/12835Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-03T13:14:31Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Sewage sludge does not induce genotoxicity and carcinogenesis
title Sewage sludge does not induce genotoxicity and carcinogenesis
spellingShingle Sewage sludge does not induce genotoxicity and carcinogenesis
Silva, Paula Regina Pereira [UNESP]
comet assay
micronucleus
sewage genotoxicity
title_short Sewage sludge does not induce genotoxicity and carcinogenesis
title_full Sewage sludge does not induce genotoxicity and carcinogenesis
title_fullStr Sewage sludge does not induce genotoxicity and carcinogenesis
title_full_unstemmed Sewage sludge does not induce genotoxicity and carcinogenesis
title_sort Sewage sludge does not induce genotoxicity and carcinogenesis
author Silva, Paula Regina Pereira [UNESP]
author_facet Silva, Paula Regina Pereira [UNESP]
Barbisan, Luis Fernando [UNESP]
Dagli, Maria Lúcia Zaidan
Saldiva, Paulo Hilário Nascimento
author_role author
author2 Barbisan, Luis Fernando [UNESP]
Dagli, Maria Lúcia Zaidan
Saldiva, Paulo Hilário Nascimento
author2_role author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
Universidade de São Paulo (USP)
dc.contributor.author.fl_str_mv Silva, Paula Regina Pereira [UNESP]
Barbisan, Luis Fernando [UNESP]
Dagli, Maria Lúcia Zaidan
Saldiva, Paulo Hilário Nascimento
dc.subject.por.fl_str_mv comet assay
micronucleus
sewage genotoxicity
topic comet assay
micronucleus
sewage genotoxicity
description Through a series of experiments, the genotoxic/mutagenic and carcinogenic potential of sewage sludge was assessed. Male Wistar rats were randomly assigned to four groups: Group 1 - negative control; Group 2 - liver carcinogenesis initiated by diethylnitrosamine (DEN; 200 mg/kg i.p.); Group 3 and G4- liver carcinogenesis initiated by DEN and fed 10,000 ppm or 50,000 ppm of sewage sludge. The animals were submitted to a 70% partial hepatectomy at the 3rd week. Livers were processed for routine histological analysis and immunohistochemistry, in order to detect glutathione S-transferase positive altered hepatocyte foci (GST-P+ AHF). Peripheral blood samples for the comet assay were obtained from the periorbital plexus immediately prior to sacrificing. Polychromatic erythrocytes (PCEs) were analyzed in femoral bone-marrow smears, and the frequencies of those micronucleated (MNPCEs) registered. There was no sewage-sludge-induced increase in frequency of either DNA damage in peripheral blood leucocytes, or MNPCEs in the femoral bone marrow. Also, there was no increase in the levels of DNA damage, in the frequency of MNPCEs, and in the development of GST-P AHF when compared with the respective control group.
publishDate 2012
dc.date.none.fl_str_mv 2012-01-01
2014-05-20T13:37:09Z
2014-05-20T13:37:09Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/S1415-47572012005000054
Genetics and Molecular Biology. Sociedade Brasileira de Genética, v. 35, n. 3, p. 657-663, 2012.
1415-4757
http://hdl.handle.net/11449/12835
10.1590/S1415-47572012005000054
S1415-47572012000400017
WOS:000307726400017
S1415-47572012000400017.pdf
3278528112652257
url http://dx.doi.org/10.1590/S1415-47572012005000054
http://hdl.handle.net/11449/12835
identifier_str_mv Genetics and Molecular Biology. Sociedade Brasileira de Genética, v. 35, n. 3, p. 657-663, 2012.
1415-4757
10.1590/S1415-47572012005000054
S1415-47572012000400017
WOS:000307726400017
S1415-47572012000400017.pdf
3278528112652257
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Genetics and Molecular Biology
1.493
0,638
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 657-663
application/pdf
dc.publisher.none.fl_str_mv Sociedade Brasileira de Genética
publisher.none.fl_str_mv Sociedade Brasileira de Genética
dc.source.none.fl_str_mv SciELO
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv repositoriounesp@unesp.br
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