Association of polymorphism of the ß(1, 4)-galactosyltransferase-I gene with milk production traits in Holsteins
Main Author: | |
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Publication Date: | 2012 |
Other Authors: | , |
Format: | Article |
Language: | eng |
Source: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Download full: | http://hdl.handle.net/10400.1/1140 |
Summary: | The b(1,4)-galactosyltransferase-I gene (b4galt1) encodes the catalytic part of the enzyme lactose synthase, responsible of lactose synthesis in the mammary gland. The complete coding region of the gene was screened for the presence of allelic variation among a sample of 1,200 Iranian Holstein cows, using PCR-SSCP technique followed by sequencing. Nine polymorphic nucleotide sites were identified- one in exons I and VI, two in exons II and III, and three in exon V. Altogether 18 different genotypes were assigned. Statistical analysis showed that the genotypes of B4GALT1 significantly affect milk, lactose, protein and total solid productions in both the first and second lactation (P\0.001). Variance component analysis considering restricted maximum likelihood showed that the major factor making differences in milk, lactose, protein and total solid productions among the studied cow is the b4galt1 genotype. We concluded that the b4galt1 gene is potentially associated with milk production traits in dairy cows and should be considered for further studies on genetics of the milk production traits. |
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Association of polymorphism of the ß(1, 4)-galactosyltransferase-I gene with milk production traits in HolsteinsGalactosyltransferaseLactose synthaseMilk production traitsCattleSNPMarker assisted selectionThe b(1,4)-galactosyltransferase-I gene (b4galt1) encodes the catalytic part of the enzyme lactose synthase, responsible of lactose synthesis in the mammary gland. The complete coding region of the gene was screened for the presence of allelic variation among a sample of 1,200 Iranian Holstein cows, using PCR-SSCP technique followed by sequencing. Nine polymorphic nucleotide sites were identified- one in exons I and VI, two in exons II and III, and three in exon V. Altogether 18 different genotypes were assigned. Statistical analysis showed that the genotypes of B4GALT1 significantly affect milk, lactose, protein and total solid productions in both the first and second lactation (P\0.001). Variance component analysis considering restricted maximum likelihood showed that the major factor making differences in milk, lactose, protein and total solid productions among the studied cow is the b4galt1 genotype. We concluded that the b4galt1 gene is potentially associated with milk production traits in dairy cows and should be considered for further studies on genetics of the milk production traits.SapientiaShahbazkia, Homayon RezaAminlari, MahmoudCravador, A.2012-05-11T15:04:26Z20122012-05-11T11:08:07Z2012-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.1/1140engShahbazkia, Homayon Reza; Aminlari, Mahmoud; Cravador, Alfredo. Association of polymorphism of the ß(1, 4)-galactosyltransferase-I gene with milk production traits in Holsteins. Molecular Biology Reports, 39, 6, 6715-6721, 2012.0301-4851AUT: HSH01524; ACR00659;info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-24T10:12:10Zoai:sapientia.ualg.pt:10400.1/1140Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:55:23.272962Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Association of polymorphism of the ß(1, 4)-galactosyltransferase-I gene with milk production traits in Holsteins |
title |
Association of polymorphism of the ß(1, 4)-galactosyltransferase-I gene with milk production traits in Holsteins |
spellingShingle |
Association of polymorphism of the ß(1, 4)-galactosyltransferase-I gene with milk production traits in Holsteins Shahbazkia, Homayon Reza Galactosyltransferase Lactose synthase Milk production traits Cattle SNP Marker assisted selection |
title_short |
Association of polymorphism of the ß(1, 4)-galactosyltransferase-I gene with milk production traits in Holsteins |
title_full |
Association of polymorphism of the ß(1, 4)-galactosyltransferase-I gene with milk production traits in Holsteins |
title_fullStr |
Association of polymorphism of the ß(1, 4)-galactosyltransferase-I gene with milk production traits in Holsteins |
title_full_unstemmed |
Association of polymorphism of the ß(1, 4)-galactosyltransferase-I gene with milk production traits in Holsteins |
title_sort |
Association of polymorphism of the ß(1, 4)-galactosyltransferase-I gene with milk production traits in Holsteins |
author |
Shahbazkia, Homayon Reza |
author_facet |
Shahbazkia, Homayon Reza Aminlari, Mahmoud Cravador, A. |
author_role |
author |
author2 |
Aminlari, Mahmoud Cravador, A. |
author2_role |
author author |
dc.contributor.none.fl_str_mv |
Sapientia |
dc.contributor.author.fl_str_mv |
Shahbazkia, Homayon Reza Aminlari, Mahmoud Cravador, A. |
dc.subject.por.fl_str_mv |
Galactosyltransferase Lactose synthase Milk production traits Cattle SNP Marker assisted selection |
topic |
Galactosyltransferase Lactose synthase Milk production traits Cattle SNP Marker assisted selection |
description |
The b(1,4)-galactosyltransferase-I gene (b4galt1) encodes the catalytic part of the enzyme lactose synthase, responsible of lactose synthesis in the mammary gland. The complete coding region of the gene was screened for the presence of allelic variation among a sample of 1,200 Iranian Holstein cows, using PCR-SSCP technique followed by sequencing. Nine polymorphic nucleotide sites were identified- one in exons I and VI, two in exons II and III, and three in exon V. Altogether 18 different genotypes were assigned. Statistical analysis showed that the genotypes of B4GALT1 significantly affect milk, lactose, protein and total solid productions in both the first and second lactation (P\0.001). Variance component analysis considering restricted maximum likelihood showed that the major factor making differences in milk, lactose, protein and total solid productions among the studied cow is the b4galt1 genotype. We concluded that the b4galt1 gene is potentially associated with milk production traits in dairy cows and should be considered for further studies on genetics of the milk production traits. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-05-11T15:04:26Z 2012 2012-05-11T11:08:07Z 2012-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.1/1140 |
url |
http://hdl.handle.net/10400.1/1140 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Shahbazkia, Homayon Reza; Aminlari, Mahmoud; Cravador, Alfredo. Association of polymorphism of the ß(1, 4)-galactosyltransferase-I gene with milk production traits in Holsteins. Molecular Biology Reports, 39, 6, 6715-6721, 2012. 0301-4851 AUT: HSH01524; ACR00659; |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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