Quantitative detection of soybean in meat products by a TaqMan real-time PCR assay

• Autor(a) principal: Soares, Sónia
• Data de Publicação: 2014
• Outros Autores: Amaral, Joana S., Oliveira, Beatriz, Mafra, Isabel
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
• Texto Completo: http://hdl.handle.net/10198/16218
• topic: Adulteration; Food allergen; Meat products; Real-time PCR; Soybean detection

In the present work, we propose a normalised real-time quantitative PCR assay to determine the addition of soybean to meat products. The method proved to be a powerful tool for the quantification of soybean protein (dry basis) in the range of 0.01% to 6%, being successfully in-house validated. Its application was effective in the analysis of several meat products, indicating 2% of non-compliance with the food allergen labelling legislation, and some inconsistencies when comparing the declared with estimated amounts of soybean. This work highlights the importance of efficient tools to assess labelling statements of meat products, avoiding fraudulent practices.