Extracellular matrix and integrins influence in the regulation of myogenic precursor cells behaviour

Detalhes bibliográficos
Autor(a) principal: Vaz, Raquel Rodrigues
Data de Publicação: 2009
Tipo de documento: Dissertação
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10451/1505
Resumo: Tese de mestrado, Biologia (Biologia Molecular Humana), 2009, Universidade de Lisboa, Faculdade de Ciências
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spelling Extracellular matrix and integrins influence in the regulation of myogenic precursor cells behaviourBiologia celularMiogéneseBiologia do desenvolvimentoIntegrinasTeses de mestradoTese de mestrado, Biologia (Biologia Molecular Humana), 2009, Universidade de Lisboa, Faculdade de CiênciasMyogenesis is the process by which undifferentiated dermomyotomal cells are specified for myogenesis, move towards the myotome where they differentiate into skeletal muscle cells that fuse into myotubes and later in development form myofibers which will constitute the skeletal muscles of the adult. The muscle precursor cells arise from the dermomyotome, an epithelial-like structure that is the source for skeletal muscle and dorsal dermis cells. Some cells, called satellite cells, go throughout part of this differentiation process but remain in a quiescent undifferentiated state (although committed to skeletal muscle fate). These cells are activated in the adult in case of muscle injury or enhanced exercise, for example. In this work we used a satellite cell-derived cell line, C2C12, and the mouse embryo to study the extracellular matrix (ECM) and integrins influence in myogenic determination and differentiation. Integrins are heterodimeric ECM receptors constituted by an α and a ß subunit that can induce, for example, migration or differentiation. The integrin ligand specificity is acquired by the combination of both subunits. Our studies have addressed that laminin-α6ß1 integrin interaction may be coordinating with Notch signaling the maintenance of undifferentiated dermomyotomal cells. By inhibiting Notch signaling, we observed precocious myogenic differentiation of dermomyotomal cells (by Myf5 expression) and the assembly of a laminin matrix around these cells. This result suggests that Myf5 induces laminin assembly. In vitro, fibronectin enhances C2C12 myoblasts alignment and migration. When we observed the myotubes of cells grown on fibronectin, we believe that the enhanced cell alignment imposed by fibronectin-α5ß1 integrin interaction will facilitate cell fusion. In vivo, we found that fibronectin is important for dermomyotome epithelial-integrity, especially through the polarization of N-cadherin, and that α5ß1 integrin signaling may also contribute to myogenic repression in the dermomyotome. These observations show that the ECM and integrins are of paramount importance in myoblast cell behaviour.Resumo alargado em português disponível no documentoRodrigues, Maria GabrielaRepositório da Universidade de LisboaVaz, Raquel Rodrigues2010-07-27T08:58:45Z20092009-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfapplication/pdfapplication/octet-streamapplication/octet-streamapplication/octet-streamapplication/octet-streamtext/xmlhttp://hdl.handle.net/10451/1505enghttp://catalogo.ul.pt/F/?func=item-global&doc_library=ULB01&type=03&doc_number=000575304info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-08T15:41:15Zoai:repositorio.ul.pt:10451/1505Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T21:28:08.052999Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Extracellular matrix and integrins influence in the regulation of myogenic precursor cells behaviour
title Extracellular matrix and integrins influence in the regulation of myogenic precursor cells behaviour
spellingShingle Extracellular matrix and integrins influence in the regulation of myogenic precursor cells behaviour
Vaz, Raquel Rodrigues
Biologia celular
Miogénese
Biologia do desenvolvimento
Integrinas
Teses de mestrado
title_short Extracellular matrix and integrins influence in the regulation of myogenic precursor cells behaviour
title_full Extracellular matrix and integrins influence in the regulation of myogenic precursor cells behaviour
title_fullStr Extracellular matrix and integrins influence in the regulation of myogenic precursor cells behaviour
title_full_unstemmed Extracellular matrix and integrins influence in the regulation of myogenic precursor cells behaviour
title_sort Extracellular matrix and integrins influence in the regulation of myogenic precursor cells behaviour
author Vaz, Raquel Rodrigues
author_facet Vaz, Raquel Rodrigues
author_role author
dc.contributor.none.fl_str_mv Rodrigues, Maria Gabriela
Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Vaz, Raquel Rodrigues
dc.subject.por.fl_str_mv Biologia celular
Miogénese
Biologia do desenvolvimento
Integrinas
Teses de mestrado
topic Biologia celular
Miogénese
Biologia do desenvolvimento
Integrinas
Teses de mestrado
description Tese de mestrado, Biologia (Biologia Molecular Humana), 2009, Universidade de Lisboa, Faculdade de Ciências
publishDate 2009
dc.date.none.fl_str_mv 2009
2009-01-01T00:00:00Z
2010-07-27T08:58:45Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10451/1505
url http://hdl.handle.net/10451/1505
dc.language.iso.fl_str_mv eng
language eng
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dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
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