Diphasic dialysis extraction technique for clenbuterol determination in bovine retina by gas chromatography-mass spectrometry

Detalhes bibliográficos
Autor(a) principal: Ramos, F.
Data de Publicação: 1999
Outros Autores: Matos, A., Oliveira, A., Silveira, M. I. Noronha da
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10316/7939
https://doi.org/10.1007/BF02493629
Resumo: Summary A method is described for the extraction of clebuterol from bovine retina samples by diphasic dialysis using gas chromatography—mass spectrometry (GC-MS) as the procedure of determination. Following chemical digestion of retinal tissue in alkaline medium at 80C, clenbuterol is extracted by diphasic dialysis using diethyl ether as solvent at 37C, for 4 h at 150 rpm. Diethyl ether is evaporated to dryness and clenbuterol determined by GC-MS after butylboronic acid (BBA) derivatization. A detection limit of 2.5 ng g-1, repeatability of 9.0% and reproducibility of 22.9%, with recoveries always >86% (n=5), are achieved as validation criteria.
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spelling Diphasic dialysis extraction technique for clenbuterol determination in bovine retina by gas chromatography-mass spectrometrySummary A method is described for the extraction of clebuterol from bovine retina samples by diphasic dialysis using gas chromatography—mass spectrometry (GC-MS) as the procedure of determination. Following chemical digestion of retinal tissue in alkaline medium at 80C, clenbuterol is extracted by diphasic dialysis using diethyl ether as solvent at 37C, for 4 h at 150 rpm. Diethyl ether is evaporated to dryness and clenbuterol determined by GC-MS after butylboronic acid (BBA) derivatization. A detection limit of 2.5 ng g-1, repeatability of 9.0% and reproducibility of 22.9%, with recoveries always >86% (n=5), are achieved as validation criteria.1999info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://hdl.handle.net/10316/7939http://hdl.handle.net/10316/7939https://doi.org/10.1007/BF02493629engChromatographia. 50:1 (1999) 118-120Ramos, F.Matos, A.Oliveira, A.Silveira, M. I. Noronha dainfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2022-05-25T03:31:57Zoai:estudogeral.uc.pt:10316/7939Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T20:47:25.412869Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Diphasic dialysis extraction technique for clenbuterol determination in bovine retina by gas chromatography-mass spectrometry
title Diphasic dialysis extraction technique for clenbuterol determination in bovine retina by gas chromatography-mass spectrometry
spellingShingle Diphasic dialysis extraction technique for clenbuterol determination in bovine retina by gas chromatography-mass spectrometry
Ramos, F.
title_short Diphasic dialysis extraction technique for clenbuterol determination in bovine retina by gas chromatography-mass spectrometry
title_full Diphasic dialysis extraction technique for clenbuterol determination in bovine retina by gas chromatography-mass spectrometry
title_fullStr Diphasic dialysis extraction technique for clenbuterol determination in bovine retina by gas chromatography-mass spectrometry
title_full_unstemmed Diphasic dialysis extraction technique for clenbuterol determination in bovine retina by gas chromatography-mass spectrometry
title_sort Diphasic dialysis extraction technique for clenbuterol determination in bovine retina by gas chromatography-mass spectrometry
author Ramos, F.
author_facet Ramos, F.
Matos, A.
Oliveira, A.
Silveira, M. I. Noronha da
author_role author
author2 Matos, A.
Oliveira, A.
Silveira, M. I. Noronha da
author2_role author
author
author
dc.contributor.author.fl_str_mv Ramos, F.
Matos, A.
Oliveira, A.
Silveira, M. I. Noronha da
description Summary A method is described for the extraction of clebuterol from bovine retina samples by diphasic dialysis using gas chromatography—mass spectrometry (GC-MS) as the procedure of determination. Following chemical digestion of retinal tissue in alkaline medium at 80C, clenbuterol is extracted by diphasic dialysis using diethyl ether as solvent at 37C, for 4 h at 150 rpm. Diethyl ether is evaporated to dryness and clenbuterol determined by GC-MS after butylboronic acid (BBA) derivatization. A detection limit of 2.5 ng g-1, repeatability of 9.0% and reproducibility of 22.9%, with recoveries always >86% (n=5), are achieved as validation criteria.
publishDate 1999
dc.date.none.fl_str_mv 1999
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10316/7939
http://hdl.handle.net/10316/7939
https://doi.org/10.1007/BF02493629
url http://hdl.handle.net/10316/7939
https://doi.org/10.1007/BF02493629
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Chromatographia. 50:1 (1999) 118-120
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
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repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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