Nanoemulsions for enhancement of curcumin bioavailability and their safety evaluation: effect of emulsifier type
Autor(a) principal: | |
---|---|
Data de Publicação: | 2021 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/71133 |
Resumo: | This work aimed at evaluating the effects of different emulsifiers on curcumin-loaded nanoemulsions behavior during digestion, its safety and absorption, to develop nanoemulsions that provide safety and improved curcumin functionality. Nanoemulsions (NEs) were produced using two bio-based (lecithin (LEC) and rhamnolipids (RHAM)) and one synthetic (Tween®80 (TWE)) emulsifier at similar concentrations. Different NEs were subjected to in vitro digestion. The cytotoxicity and permeability tests were performed in Caco-2 cells. NE_TWE were stable during all phases of in vitro digestion, whereas NE_LEC and NE_RHAM were found to be unstable from the gastric phase. NE_TWE showed 100% of free fatty acids released, followed by NE_RHAM and NE_LEC. Curcumins bioaccessibility and stability increased in the following order: NE_LEC > NE_RHAM > NE_TWE. NE_LEC and NE_TWE did not show cytotoxic effects in any of the concentrations tested, while NE_RHAM presented high cytotoxicity in all concentrations tested. The apparent permeability coefficients were determined for NE_LEC and NE_TWE; however, the results were not statistically different. These results showed that the emulsifier used has a high impact on nanoemulsions behavior under the digestion process and on their cytotoxicity. This work contributed to the state-of-the-arts progress on the development of safer curcumin delivery systems with improved functionality, particularly regarding the proper selection of ingredients to produce said systems. |
id |
RCAP_c4158581e5fcdc76ec12a4194accf291 |
---|---|
oai_identifier_str |
oai:repositorium.sdum.uminho.pt:1822/71133 |
network_acronym_str |
RCAP |
network_name_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository_id_str |
7160 |
spelling |
Nanoemulsions for enhancement of curcumin bioavailability and their safety evaluation: effect of emulsifier typenanoemulsionslecithinrhamnolipidsTween® 80In vitro static digestioncytotoxicityTween(®)80Science & TechnologyThis work aimed at evaluating the effects of different emulsifiers on curcumin-loaded nanoemulsions behavior during digestion, its safety and absorption, to develop nanoemulsions that provide safety and improved curcumin functionality. Nanoemulsions (NEs) were produced using two bio-based (lecithin (LEC) and rhamnolipids (RHAM)) and one synthetic (Tween®80 (TWE)) emulsifier at similar concentrations. Different NEs were subjected to in vitro digestion. The cytotoxicity and permeability tests were performed in Caco-2 cells. NE_TWE were stable during all phases of in vitro digestion, whereas NE_LEC and NE_RHAM were found to be unstable from the gastric phase. NE_TWE showed 100% of free fatty acids released, followed by NE_RHAM and NE_LEC. Curcumins bioaccessibility and stability increased in the following order: NE_LEC > NE_RHAM > NE_TWE. NE_LEC and NE_TWE did not show cytotoxic effects in any of the concentrations tested, while NE_RHAM presented high cytotoxicity in all concentrations tested. The apparent permeability coefficients were determined for NE_LEC and NE_TWE; however, the results were not statistically different. These results showed that the emulsifier used has a high impact on nanoemulsions behavior under the digestion process and on their cytotoxicity. This work contributed to the state-of-the-arts progress on the development of safer curcumin delivery systems with improved functionality, particularly regarding the proper selection of ingredients to produce said systems.This study was supported by the Portuguese Foundation for Science and Technology (FCT) under the scope of the strategic funding of UIDB/04469/2020 unit and under the scope of project PTDC/AGR-TEC/5215/2014.info:eu-repo/semantics/publishedVersionMDPIUniversidade do MinhoGonçalves, Raquel Filipa SilvaMartins, Joana T.Abrunhosa, LuísVicente, A. A.Pinheiro, A. C.20212021-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/71133engGonçalves, Raquel F. S.; Martins, Joana T.; Abrunhosa, Luís; Vicente, António A.; Pinheiro, Ana Cristina, Nanoemulsions for enhancement of curcumin bioavailability and their safety evaluation: effect of emulsifier type. Nanomaterials, 11(3), 815, 20212079499110.3390/nano11030815https://www.mdpi.com/2079-4991/11/3/815info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:14:45Zoai:repositorium.sdum.uminho.pt:1822/71133Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:07:05.679595Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Nanoemulsions for enhancement of curcumin bioavailability and their safety evaluation: effect of emulsifier type |
title |
Nanoemulsions for enhancement of curcumin bioavailability and their safety evaluation: effect of emulsifier type |
spellingShingle |
Nanoemulsions for enhancement of curcumin bioavailability and their safety evaluation: effect of emulsifier type Gonçalves, Raquel Filipa Silva nanoemulsions lecithin rhamnolipids Tween® 80 In vitro static digestion cytotoxicity Tween(®) 80 Science & Technology |
title_short |
Nanoemulsions for enhancement of curcumin bioavailability and their safety evaluation: effect of emulsifier type |
title_full |
Nanoemulsions for enhancement of curcumin bioavailability and their safety evaluation: effect of emulsifier type |
title_fullStr |
Nanoemulsions for enhancement of curcumin bioavailability and their safety evaluation: effect of emulsifier type |
title_full_unstemmed |
Nanoemulsions for enhancement of curcumin bioavailability and their safety evaluation: effect of emulsifier type |
title_sort |
Nanoemulsions for enhancement of curcumin bioavailability and their safety evaluation: effect of emulsifier type |
author |
Gonçalves, Raquel Filipa Silva |
author_facet |
Gonçalves, Raquel Filipa Silva Martins, Joana T. Abrunhosa, Luís Vicente, A. A. Pinheiro, A. C. |
author_role |
author |
author2 |
Martins, Joana T. Abrunhosa, Luís Vicente, A. A. Pinheiro, A. C. |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Gonçalves, Raquel Filipa Silva Martins, Joana T. Abrunhosa, Luís Vicente, A. A. Pinheiro, A. C. |
dc.subject.por.fl_str_mv |
nanoemulsions lecithin rhamnolipids Tween® 80 In vitro static digestion cytotoxicity Tween(®) 80 Science & Technology |
topic |
nanoemulsions lecithin rhamnolipids Tween® 80 In vitro static digestion cytotoxicity Tween(®) 80 Science & Technology |
description |
This work aimed at evaluating the effects of different emulsifiers on curcumin-loaded nanoemulsions behavior during digestion, its safety and absorption, to develop nanoemulsions that provide safety and improved curcumin functionality. Nanoemulsions (NEs) were produced using two bio-based (lecithin (LEC) and rhamnolipids (RHAM)) and one synthetic (Tween®80 (TWE)) emulsifier at similar concentrations. Different NEs were subjected to in vitro digestion. The cytotoxicity and permeability tests were performed in Caco-2 cells. NE_TWE were stable during all phases of in vitro digestion, whereas NE_LEC and NE_RHAM were found to be unstable from the gastric phase. NE_TWE showed 100% of free fatty acids released, followed by NE_RHAM and NE_LEC. Curcumins bioaccessibility and stability increased in the following order: NE_LEC > NE_RHAM > NE_TWE. NE_LEC and NE_TWE did not show cytotoxic effects in any of the concentrations tested, while NE_RHAM presented high cytotoxicity in all concentrations tested. The apparent permeability coefficients were determined for NE_LEC and NE_TWE; however, the results were not statistically different. These results showed that the emulsifier used has a high impact on nanoemulsions behavior under the digestion process and on their cytotoxicity. This work contributed to the state-of-the-arts progress on the development of safer curcumin delivery systems with improved functionality, particularly regarding the proper selection of ingredients to produce said systems. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021 2021-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/71133 |
url |
http://hdl.handle.net/1822/71133 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Gonçalves, Raquel F. S.; Martins, Joana T.; Abrunhosa, Luís; Vicente, António A.; Pinheiro, Ana Cristina, Nanoemulsions for enhancement of curcumin bioavailability and their safety evaluation: effect of emulsifier type. Nanomaterials, 11(3), 815, 2021 20794991 10.3390/nano11030815 https://www.mdpi.com/2079-4991/11/3/815 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
MDPI |
publisher.none.fl_str_mv |
MDPI |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
|
_version_ |
1799132488727003136 |