Microevolution of a Mycobacteroides abscessus subsp. bolletii strain in a clinical persistent infection

Detalhes bibliográficos
Autor(a) principal: Santos, Andrea
Data de Publicação: 2023
Outros Autores: Pinto, Miguel, Carneiro, Sofia, Silva, Sónia, Rodrigues, Irene, Munhá, João, Gomes, João Paulo, Macedo, Rita
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/159840
Resumo: Publisher Copyright: © 2023 The Authors
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spelling Microevolution of a Mycobacteroides abscessus subsp. bolletii strain in a clinical persistent infectionGenomic evolutionMycobacterium abscessusMycobacterium bolletiiNontuberculous mycobacteriaPersistent infectionMicrobiologyEcology, Evolution, Behavior and SystematicsMolecular BiologyGeneticsMicrobiology (medical)Infectious DiseasesSDG 3 - Good Health and Well-beingPublisher Copyright: © 2023 The AuthorsMycobacteroides abscessus complex (MAB), a fast-growing nontuberculous mycobacterium, is emerging as a significant infectious disease threat, due to both intrinsic and acquired resistance mechanisms to antibiotics and disinfectants and the need for extensive and multidrug regimens for treatment. Despite the prolonged regimens, outcomes are poor and persistence cases have been reported. Here, we describe clinical, microbiologic and genomic features of a M. abscessus subsp. bolletii (M. bolletii) strain consecutively isolated from a patient within an eight-year infection period. From April 2014 to September 2021, the National Reference Laboratory for Mycobacteria received eight strains isolated from a male patient. Species identification, molecular resistance profile and phenotypic drug susceptibility were determined. Five of these isolates were recovered for further in-depth genomic analysis. Genomic analysis confirmed the multidrug resistant pattern of the strain and also other genetic changes associated with adaptation to environment and defence mechanisms. We highlight the identification of new mutations in locus MAB_1881c and in locus MAB_4099c (mps1 gene), already described as associated with macrolides resistance and morphotype switching, respectively. Additionally, we also observed the emergence and fixation of a mutation in locus MAB_0364c that appeared at a frequency of 36% for the 2014 isolate, 57% for the 2015 isolate and 100% for the 2017 and 2021 isolates, clearly illustrating a fixation process underlying a microevolution of the MAB strain within the patient. Altogether these results suggest that the observed genetic alterations are a reflection of the bacterial population's continuous adaptation and survival to the host environment during infection, contributing to persistence and treatment failure.DCV - Departamento de Ciências da VidaRUNSantos, AndreaPinto, MiguelCarneiro, SofiaSilva, SóniaRodrigues, IreneMunhá, JoãoGomes, João PauloMacedo, Rita2023-11-10T22:12:02Z2023-082023-08-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article6application/pdfhttp://hdl.handle.net/10362/159840engSantos, A., Pinto, M., Carneiro, S., Silva, S., Rodrigues, I., Munhá, J., Gomes, J. P., & Macedo, R. (2023). Microevolution of a Mycobacteroides abscessus subsp. bolletii strain in a clinical persistent infection. Infection, Genetics and Evolution, 112, [105437]. https://doi.org/10.1016/j.meegid.2023.1054371567-1348PURE: 75830518https://doi.org/10.1016/j.meegid.2023.105437info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T05:42:21Zoai:run.unl.pt:10362/159840Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:57:44.844279Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Microevolution of a Mycobacteroides abscessus subsp. bolletii strain in a clinical persistent infection
title Microevolution of a Mycobacteroides abscessus subsp. bolletii strain in a clinical persistent infection
spellingShingle Microevolution of a Mycobacteroides abscessus subsp. bolletii strain in a clinical persistent infection
Santos, Andrea
Genomic evolution
Mycobacterium abscessus
Mycobacterium bolletii
Nontuberculous mycobacteria
Persistent infection
Microbiology
Ecology, Evolution, Behavior and Systematics
Molecular Biology
Genetics
Microbiology (medical)
Infectious Diseases
SDG 3 - Good Health and Well-being
title_short Microevolution of a Mycobacteroides abscessus subsp. bolletii strain in a clinical persistent infection
title_full Microevolution of a Mycobacteroides abscessus subsp. bolletii strain in a clinical persistent infection
title_fullStr Microevolution of a Mycobacteroides abscessus subsp. bolletii strain in a clinical persistent infection
title_full_unstemmed Microevolution of a Mycobacteroides abscessus subsp. bolletii strain in a clinical persistent infection
title_sort Microevolution of a Mycobacteroides abscessus subsp. bolletii strain in a clinical persistent infection
author Santos, Andrea
author_facet Santos, Andrea
Pinto, Miguel
Carneiro, Sofia
Silva, Sónia
Rodrigues, Irene
Munhá, João
Gomes, João Paulo
Macedo, Rita
author_role author
author2 Pinto, Miguel
Carneiro, Sofia
Silva, Sónia
Rodrigues, Irene
Munhá, João
Gomes, João Paulo
Macedo, Rita
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv DCV - Departamento de Ciências da Vida
RUN
dc.contributor.author.fl_str_mv Santos, Andrea
Pinto, Miguel
Carneiro, Sofia
Silva, Sónia
Rodrigues, Irene
Munhá, João
Gomes, João Paulo
Macedo, Rita
dc.subject.por.fl_str_mv Genomic evolution
Mycobacterium abscessus
Mycobacterium bolletii
Nontuberculous mycobacteria
Persistent infection
Microbiology
Ecology, Evolution, Behavior and Systematics
Molecular Biology
Genetics
Microbiology (medical)
Infectious Diseases
SDG 3 - Good Health and Well-being
topic Genomic evolution
Mycobacterium abscessus
Mycobacterium bolletii
Nontuberculous mycobacteria
Persistent infection
Microbiology
Ecology, Evolution, Behavior and Systematics
Molecular Biology
Genetics
Microbiology (medical)
Infectious Diseases
SDG 3 - Good Health and Well-being
description Publisher Copyright: © 2023 The Authors
publishDate 2023
dc.date.none.fl_str_mv 2023-11-10T22:12:02Z
2023-08
2023-08-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/159840
url http://hdl.handle.net/10362/159840
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Santos, A., Pinto, M., Carneiro, S., Silva, S., Rodrigues, I., Munhá, J., Gomes, J. P., & Macedo, R. (2023). Microevolution of a Mycobacteroides abscessus subsp. bolletii strain in a clinical persistent infection. Infection, Genetics and Evolution, 112, [105437]. https://doi.org/10.1016/j.meegid.2023.105437
1567-1348
PURE: 75830518
https://doi.org/10.1016/j.meegid.2023.105437
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 6
application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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