Integrin-specific hydrogels for growth factor-free vasculogenesis

Detalhes bibliográficos
Autor(a) principal: Moreira, Helena R.
Data de Publicação: 2022
Outros Autores: Rodrigues, Daniel Barreira, Ribeiro, Sara Freitas, Silva, Lucília Pereira, Morais, Alain da S., Jarnalo, Mariana, Horta, Ricardo, Reis, R. L., Pirraco, Rogério P., Marques, A. P.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: https://hdl.handle.net/1822/81629
Resumo: Integrin-binding biomaterials have been extensively evaluated for their capacity to enable de novo formation of capillary-like structures/vessels, ultimately supporting neovascularization in vivo. Yet, the role of integrins as vascular initiators in engineered materials is still not well understood. Here, we show that αvβ3 integrin-specific 3D matrices were able to retain PECAM1+ cells from the stromal vascular fraction (SVF) of adipose tissue, triggering vasculogenesis in vitro in the absence of extrinsic growth factors. Our results suggest that αvβ3-RGD-driven signaling in the formation of capillary-like structures prevents the activation of the caspase 8 pathway and activates the FAK/paxillin pathway, both responsible for endothelial cells (ECs) survival and migration. We also show that prevascularized αvβ3 integrin-specific constructs inosculate with the host vascular system fostering in vivo neovascularization. Overall, this work demonstrates the ability of the biomaterial to trigger vasculogenesis in an integrin-specific manner, by activating essential pathways for EC survival and migration within a self-regulatory growth factor microenvironment. This strategy represents an improvement to current vascularization routes for Tissue Engineering constructs, potentially enhancing their clinical applicability.
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spelling Integrin-specific hydrogels for growth factor-free vasculogenesisBiomaterialsStromal vascular fractionVascularizationVasculogenesisScience & TechnologyIntegrin-binding biomaterials have been extensively evaluated for their capacity to enable de novo formation of capillary-like structures/vessels, ultimately supporting neovascularization in vivo. Yet, the role of integrins as vascular initiators in engineered materials is still not well understood. Here, we show that αvβ3 integrin-specific 3D matrices were able to retain PECAM1+ cells from the stromal vascular fraction (SVF) of adipose tissue, triggering vasculogenesis in vitro in the absence of extrinsic growth factors. Our results suggest that αvβ3-RGD-driven signaling in the formation of capillary-like structures prevents the activation of the caspase 8 pathway and activates the FAK/paxillin pathway, both responsible for endothelial cells (ECs) survival and migration. We also show that prevascularized αvβ3 integrin-specific constructs inosculate with the host vascular system fostering in vivo neovascularization. Overall, this work demonstrates the ability of the biomaterial to trigger vasculogenesis in an integrin-specific manner, by activating essential pathways for EC survival and migration within a self-regulatory growth factor microenvironment. This strategy represents an improvement to current vascularization routes for Tissue Engineering constructs, potentially enhancing their clinical applicability.The authors would like to acknowledge the financial support from the Consolidator Grant “ECM_INK” (ERC-2016-COG-726061) and the Starting Grant “CapBed” (ERC2018-STG-805411), to the FSE/POCH (Fundo Social Europeu através do Programa Operacional do Capital Humano) under the scope of the PD/169/2013, NORTE-08- 5369-FSE-000037 (H.R.M.), and to FCT/MCTES (Fundação para a Ciência e a Tecnologia/ Ministério da Ciência, Tecnologia, e Ensino Superior) through the grants SFRH/BD/119756/2016 (D.B.R.), Ph.D. grant PD/BD/135252/2017 (S.F.R.) and IF/00347/ 2015 (R.P.P.).Springer NatureUniversidade do MinhoMoreira, Helena R.Rodrigues, Daniel BarreiraRibeiro, Sara FreitasSilva, Lucília PereiraMorais, Alain da S.Jarnalo, MarianaHorta, RicardoReis, R. L.Pirraco, Rogério P.Marques, A. P.2022-092022-09-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/1822/81629engMoreira H. R., Rodrigues D. B., Freitas-Ribeiro S., da Silva L. P., Morais A., Jalano M., Horta R., Reis R. L., Pirraco R. P., Marques A. P. Integrin-specific hydrogels for growth factor-free vasculogenesis, npj Regenerative Medicine, Vol. 7, pp. 57, doi:10.1038/s41536-022-00253-4, 20222057-399510.1038/s41536-022-00253-457https://www.nature.com/articles/s41536-022-00253-4info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:44:20Zoai:repositorium.sdum.uminho.pt:1822/81629Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:42:00.618019Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Integrin-specific hydrogels for growth factor-free vasculogenesis
title Integrin-specific hydrogels for growth factor-free vasculogenesis
spellingShingle Integrin-specific hydrogels for growth factor-free vasculogenesis
Moreira, Helena R.
Biomaterials
Stromal vascular fraction
Vascularization
Vasculogenesis
Science & Technology
title_short Integrin-specific hydrogels for growth factor-free vasculogenesis
title_full Integrin-specific hydrogels for growth factor-free vasculogenesis
title_fullStr Integrin-specific hydrogels for growth factor-free vasculogenesis
title_full_unstemmed Integrin-specific hydrogels for growth factor-free vasculogenesis
title_sort Integrin-specific hydrogels for growth factor-free vasculogenesis
author Moreira, Helena R.
author_facet Moreira, Helena R.
Rodrigues, Daniel Barreira
Ribeiro, Sara Freitas
Silva, Lucília Pereira
Morais, Alain da S.
Jarnalo, Mariana
Horta, Ricardo
Reis, R. L.
Pirraco, Rogério P.
Marques, A. P.
author_role author
author2 Rodrigues, Daniel Barreira
Ribeiro, Sara Freitas
Silva, Lucília Pereira
Morais, Alain da S.
Jarnalo, Mariana
Horta, Ricardo
Reis, R. L.
Pirraco, Rogério P.
Marques, A. P.
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Moreira, Helena R.
Rodrigues, Daniel Barreira
Ribeiro, Sara Freitas
Silva, Lucília Pereira
Morais, Alain da S.
Jarnalo, Mariana
Horta, Ricardo
Reis, R. L.
Pirraco, Rogério P.
Marques, A. P.
dc.subject.por.fl_str_mv Biomaterials
Stromal vascular fraction
Vascularization
Vasculogenesis
Science & Technology
topic Biomaterials
Stromal vascular fraction
Vascularization
Vasculogenesis
Science & Technology
description Integrin-binding biomaterials have been extensively evaluated for their capacity to enable de novo formation of capillary-like structures/vessels, ultimately supporting neovascularization in vivo. Yet, the role of integrins as vascular initiators in engineered materials is still not well understood. Here, we show that αvβ3 integrin-specific 3D matrices were able to retain PECAM1+ cells from the stromal vascular fraction (SVF) of adipose tissue, triggering vasculogenesis in vitro in the absence of extrinsic growth factors. Our results suggest that αvβ3-RGD-driven signaling in the formation of capillary-like structures prevents the activation of the caspase 8 pathway and activates the FAK/paxillin pathway, both responsible for endothelial cells (ECs) survival and migration. We also show that prevascularized αvβ3 integrin-specific constructs inosculate with the host vascular system fostering in vivo neovascularization. Overall, this work demonstrates the ability of the biomaterial to trigger vasculogenesis in an integrin-specific manner, by activating essential pathways for EC survival and migration within a self-regulatory growth factor microenvironment. This strategy represents an improvement to current vascularization routes for Tissue Engineering constructs, potentially enhancing their clinical applicability.
publishDate 2022
dc.date.none.fl_str_mv 2022-09
2022-09-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://hdl.handle.net/1822/81629
url https://hdl.handle.net/1822/81629
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Moreira H. R., Rodrigues D. B., Freitas-Ribeiro S., da Silva L. P., Morais A., Jalano M., Horta R., Reis R. L., Pirraco R. P., Marques A. P. Integrin-specific hydrogels for growth factor-free vasculogenesis, npj Regenerative Medicine, Vol. 7, pp. 57, doi:10.1038/s41536-022-00253-4, 2022
2057-3995
10.1038/s41536-022-00253-4
57
https://www.nature.com/articles/s41536-022-00253-4
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Springer Nature
publisher.none.fl_str_mv Springer Nature
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
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