Post-transcriptional silencing of Bos taurus prion family genes and its impact on granulosa cell steroidogenesis

Detalhes bibliográficos
Autor(a) principal: Pimenta, Jorge M.
Data de Publicação: 2022
Outros Autores: Pires, Virgínia M., Nolasco, Sofia, Castelo-Branco, Pedro, Marques, Carla C., Apolónio, Joana, Azevedo, Rita, Fernandes, Mónica T., Lopes-da-Costa, Luís, Prates, José, Pereira, Rosa M.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.21/14284
Resumo: FCT_UIDB/00276/2020
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spelling Post-transcriptional silencing of Bos taurus prion family genes and its impact on granulosa cell steroidogenesisBovine granulosa cellsPrion-like genessiRNASteroidogenesisFCT_UIDB/00276/2020ALT20-03-0246-FEDER-000021FCT_UIDB/00276/2020ALT20-03-0246-FEDER-000021Prion proteins constitute a major public health concern, which has partly overshadowed their physiological roles in several scenarios. Indeed, these proteins were implicated in male fertility but their role in female fertility is relatively less explored. This study was designed to evaluate the role of SPRN and PRNP prion family genes in bovine follicular steroidogenesis pathways. Post-transcriptional SPRN and PRNP silencing with siRNAs was established in bovine granulosa cell (GC) in vitro culture, and gene expression and progesterone and estradiol concentrations were evaluated. SPRN knockdown, led to a downregulation of CYP11A1 mRNA levels (2.1-fold), and PRNP knockdown led to an upregulation of SPRN mRNA levels (2.3-fold). CYP19A1 expression and estradiol synthesis was not detected in any experimental group. Finally, SPRN knockdown led to a mild reduction in progesterone production in GCs and this was the only experimental group that did not exhibit an increment in progesterone levels after 48 h of culture. As a conclusion, it was possible to detect the expression of the SPRN gene in bovine GCs, a potential interaction between SPRN and PRNP regulation, and the impact of SPRN expression on CYP11A1 and progesterone levels. These findings bring new insights into the role of these genes in ovarian steroidogenesis and female reproductive physiology.ElsevierRCIPLPimenta, Jorge M.Pires, Virgínia M.Nolasco, SofiaCastelo-Branco, PedroMarques, Carla C.Apolónio, JoanaAzevedo, RitaFernandes, Mónica T.Lopes-da-Costa, LuísPrates, JoséPereira, Rosa M.2022-042022-04-01T00:00:00Z2024-04-30T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.21/14284engPimenta JM, Pires MR, Nolasco S, Castelo-Branco P, Marques CC, Apolónio J, et al. Post-transcriptional silencing of Bos taurus prion family genes and its impact on granulosa cell steroidogenesis. Biochem Biophys Res Commun. 2022;598:95-9.10.1016/j.bbrc.2022.02.012info:eu-repo/semantics/embargoedAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-08-03T10:10:13Zoai:repositorio.ipl.pt:10400.21/14284Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T20:22:06.923512Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Post-transcriptional silencing of Bos taurus prion family genes and its impact on granulosa cell steroidogenesis
title Post-transcriptional silencing of Bos taurus prion family genes and its impact on granulosa cell steroidogenesis
spellingShingle Post-transcriptional silencing of Bos taurus prion family genes and its impact on granulosa cell steroidogenesis
Pimenta, Jorge M.
Bovine granulosa cells
Prion-like genes
siRNA
Steroidogenesis
FCT_UIDB/00276/2020
ALT20-03-0246-FEDER-000021
title_short Post-transcriptional silencing of Bos taurus prion family genes and its impact on granulosa cell steroidogenesis
title_full Post-transcriptional silencing of Bos taurus prion family genes and its impact on granulosa cell steroidogenesis
title_fullStr Post-transcriptional silencing of Bos taurus prion family genes and its impact on granulosa cell steroidogenesis
title_full_unstemmed Post-transcriptional silencing of Bos taurus prion family genes and its impact on granulosa cell steroidogenesis
title_sort Post-transcriptional silencing of Bos taurus prion family genes and its impact on granulosa cell steroidogenesis
author Pimenta, Jorge M.
author_facet Pimenta, Jorge M.
Pires, Virgínia M.
Nolasco, Sofia
Castelo-Branco, Pedro
Marques, Carla C.
Apolónio, Joana
Azevedo, Rita
Fernandes, Mónica T.
Lopes-da-Costa, Luís
Prates, José
Pereira, Rosa M.
author_role author
author2 Pires, Virgínia M.
Nolasco, Sofia
Castelo-Branco, Pedro
Marques, Carla C.
Apolónio, Joana
Azevedo, Rita
Fernandes, Mónica T.
Lopes-da-Costa, Luís
Prates, José
Pereira, Rosa M.
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv RCIPL
dc.contributor.author.fl_str_mv Pimenta, Jorge M.
Pires, Virgínia M.
Nolasco, Sofia
Castelo-Branco, Pedro
Marques, Carla C.
Apolónio, Joana
Azevedo, Rita
Fernandes, Mónica T.
Lopes-da-Costa, Luís
Prates, José
Pereira, Rosa M.
dc.subject.por.fl_str_mv Bovine granulosa cells
Prion-like genes
siRNA
Steroidogenesis
FCT_UIDB/00276/2020
ALT20-03-0246-FEDER-000021
topic Bovine granulosa cells
Prion-like genes
siRNA
Steroidogenesis
FCT_UIDB/00276/2020
ALT20-03-0246-FEDER-000021
description FCT_UIDB/00276/2020
publishDate 2022
dc.date.none.fl_str_mv 2022-04
2022-04-01T00:00:00Z
2024-04-30T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.21/14284
url http://hdl.handle.net/10400.21/14284
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Pimenta JM, Pires MR, Nolasco S, Castelo-Branco P, Marques CC, Apolónio J, et al. Post-transcriptional silencing of Bos taurus prion family genes and its impact on granulosa cell steroidogenesis. Biochem Biophys Res Commun. 2022;598:95-9.
10.1016/j.bbrc.2022.02.012
dc.rights.driver.fl_str_mv info:eu-repo/semantics/embargoedAccess
eu_rights_str_mv embargoedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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