Culture media and sampling collection method for Aspergillus spp. assessment : tackling the gap between recommendations and the scientific evidence

Detalhes bibliográficos
Autor(a) principal: Viegas, Carla
Data de Publicação: 2020
Outros Autores: Dias, Marta, Carolino, Elisabete, Sabino, Raquel Filipa Pinheiro
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10451/45689
Resumo: Copyright: © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license.
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spelling Culture media and sampling collection method for Aspergillus spp. assessment : tackling the gap between recommendations and the scientific evidenceExposureMEADG18SamplingIndoorAspergillus spp.Copyright: © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license.Culturing is still the most widely used method for determining fungal growth. Thus, is important to identify the most suitable culture media to assess Aspergillus spp. The aim of this study was to analyze data obtained from previous studies, aiming at identifying the most suitable culture media (malt extract agar (MEA) or dichloran-glycerol agar (DG18) to assess Aspergillus spp. isolation and growth. This study was conducted by using environmental samples (n = 1153). Most of the active sampling methods (air samples) were impacted directly onto both culture media. As for passive sampling methods, fungi were extracted from environmental matrices inoculated onto both media. Overall, total Aspergillus counts were higher in MEA (n = 617, 53.5%) than in DG18 (n = 536, 46.5%). Regarding Aspergillus sections, significant associations were detected with the media (χ2 (7) = 241.118, p < 0.001), the sampling approach (p < 0.001, 95% CI = (0.3 × 10−4), and the indoor environment (p < 0.001, 95% CI = (0.3 × 10−4)). As such, sampling approach and the culture media should be accurately selected when dealing with Aspergillus spp. exposure assessment.This work was supported by FCT—Fundação para Ciência e Tecnologia for funding the project EXPOsE—Establishing protocols to assess occupational exposure to microbiota in clinical settings (02/SAICT/2016—Project n° 23222) and Instituto Politécnico de Lisboa, Lisbon, Portugal for funding the Project “Waste Workers’ Exposure to Bio-burden through Filtering Respiratory Protective Devices” (IPL/2018/WasteFRPD_ESTeSL) and the Project “Occupational exposure of ambulance drivers to bioburden” (IPL/2020/BIO-AmbuDrivers_ESTeSL). H&TRC authors gratefully acknowledge the FCT/MCTES national support through the UIDB/05608/2020 and UIDP/05608/2020.MDPIRepositório da Universidade de LisboaViegas, CarlaDias, MartaCarolino, ElisabeteSabino, Raquel Filipa Pinheiro2021-01-07T12:40:01Z20202020-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10451/45689engAtmosphere 2021, 12(1), 2310.3390/atmos120100232073-4433info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-08T16:47:39Zoai:repositorio.ul.pt:10451/45689Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T21:58:03.255376Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Culture media and sampling collection method for Aspergillus spp. assessment : tackling the gap between recommendations and the scientific evidence
title Culture media and sampling collection method for Aspergillus spp. assessment : tackling the gap between recommendations and the scientific evidence
spellingShingle Culture media and sampling collection method for Aspergillus spp. assessment : tackling the gap between recommendations and the scientific evidence
Viegas, Carla
Exposure
MEA
DG18
Sampling
Indoor
Aspergillus spp.
title_short Culture media and sampling collection method for Aspergillus spp. assessment : tackling the gap between recommendations and the scientific evidence
title_full Culture media and sampling collection method for Aspergillus spp. assessment : tackling the gap between recommendations and the scientific evidence
title_fullStr Culture media and sampling collection method for Aspergillus spp. assessment : tackling the gap between recommendations and the scientific evidence
title_full_unstemmed Culture media and sampling collection method for Aspergillus spp. assessment : tackling the gap between recommendations and the scientific evidence
title_sort Culture media and sampling collection method for Aspergillus spp. assessment : tackling the gap between recommendations and the scientific evidence
author Viegas, Carla
author_facet Viegas, Carla
Dias, Marta
Carolino, Elisabete
Sabino, Raquel Filipa Pinheiro
author_role author
author2 Dias, Marta
Carolino, Elisabete
Sabino, Raquel Filipa Pinheiro
author2_role author
author
author
dc.contributor.none.fl_str_mv Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Viegas, Carla
Dias, Marta
Carolino, Elisabete
Sabino, Raquel Filipa Pinheiro
dc.subject.por.fl_str_mv Exposure
MEA
DG18
Sampling
Indoor
Aspergillus spp.
topic Exposure
MEA
DG18
Sampling
Indoor
Aspergillus spp.
description Copyright: © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license.
publishDate 2020
dc.date.none.fl_str_mv 2020
2020-01-01T00:00:00Z
2021-01-07T12:40:01Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10451/45689
url http://hdl.handle.net/10451/45689
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Atmosphere 2021, 12(1), 23
10.3390/atmos12010023
2073-4433
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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