Culture media and sampling collection method for aspergillus spp. Assessment
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10362/111106 |
Resumo: | Culturing is still the most widely used method for determining fungal growth. Thus, is important to identify the most suitable culture media to assess Aspergillus spp. The aim of this study was to analyze data obtained from previous studies, aiming at identifying the most suitable culture media (malt extract agar (MEA) or dichloran-glycerol agar (DG18) to assess Aspergillus spp. isolation and growth. This study was conducted by using environmental samples (n = 1153). Most of the active sampling methods (air samples) were impacted directly onto both culture media. As for passive sampling methods, fungi were extracted from environmental matrices inoculated onto both media. Overall, total Aspergillus counts were higher in MEA (n = 617, 53.5%) than in DG18 (n = 536, 46.5%). Regarding Aspergillus sections, significant associations were detected with the media (χ2 (7) = 241.118, p < 0.001), the sampling approach (p < 0.001, 95% CI = (0.3 × 10−4 ), and the indoor environment (p < 0.001, 95% CI = (0.3 × 10−4 )). As such, sampling approach and the culture media should be accurately selected when dealing with Aspergillus spp. exposure assessment. |
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Culture media and sampling collection method for aspergillus spp. Assessmenttackling the gap between recommendations and the scientific evidenceAspergillus sppDG18ExposureIndoorMEASamplingEnvironmental Science (miscellaneous)Culturing is still the most widely used method for determining fungal growth. Thus, is important to identify the most suitable culture media to assess Aspergillus spp. The aim of this study was to analyze data obtained from previous studies, aiming at identifying the most suitable culture media (malt extract agar (MEA) or dichloran-glycerol agar (DG18) to assess Aspergillus spp. isolation and growth. This study was conducted by using environmental samples (n = 1153). Most of the active sampling methods (air samples) were impacted directly onto both culture media. As for passive sampling methods, fungi were extracted from environmental matrices inoculated onto both media. Overall, total Aspergillus counts were higher in MEA (n = 617, 53.5%) than in DG18 (n = 536, 46.5%). Regarding Aspergillus sections, significant associations were detected with the media (χ2 (7) = 241.118, p < 0.001), the sampling approach (p < 0.001, 95% CI = (0.3 × 10−4 ), and the indoor environment (p < 0.001, 95% CI = (0.3 × 10−4 )). As such, sampling approach and the culture media should be accurately selected when dealing with Aspergillus spp. exposure assessment.Comprehensive Health Research Centre (CHRC) - Pólo ENSPCentro de Investigação em Saúde Pública (CISP/PHRC)RUNViegas, CarlaDias, MartaCarolino, ElisabeteSabino, Raquel2021-02-01T23:31:58Z2021-012021-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article12application/pdfhttp://hdl.handle.net/10362/111106eng2073-4433PURE: 27817615https://doi.org/10.3390/atmos12010023info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T04:54:53Zoai:run.unl.pt:10362/111106Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:41:47.640339Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Culture media and sampling collection method for aspergillus spp. Assessment tackling the gap between recommendations and the scientific evidence |
title |
Culture media and sampling collection method for aspergillus spp. Assessment |
spellingShingle |
Culture media and sampling collection method for aspergillus spp. Assessment Viegas, Carla Aspergillus spp DG18 Exposure Indoor MEA Sampling Environmental Science (miscellaneous) |
title_short |
Culture media and sampling collection method for aspergillus spp. Assessment |
title_full |
Culture media and sampling collection method for aspergillus spp. Assessment |
title_fullStr |
Culture media and sampling collection method for aspergillus spp. Assessment |
title_full_unstemmed |
Culture media and sampling collection method for aspergillus spp. Assessment |
title_sort |
Culture media and sampling collection method for aspergillus spp. Assessment |
author |
Viegas, Carla |
author_facet |
Viegas, Carla Dias, Marta Carolino, Elisabete Sabino, Raquel |
author_role |
author |
author2 |
Dias, Marta Carolino, Elisabete Sabino, Raquel |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Comprehensive Health Research Centre (CHRC) - Pólo ENSP Centro de Investigação em Saúde Pública (CISP/PHRC) RUN |
dc.contributor.author.fl_str_mv |
Viegas, Carla Dias, Marta Carolino, Elisabete Sabino, Raquel |
dc.subject.por.fl_str_mv |
Aspergillus spp DG18 Exposure Indoor MEA Sampling Environmental Science (miscellaneous) |
topic |
Aspergillus spp DG18 Exposure Indoor MEA Sampling Environmental Science (miscellaneous) |
description |
Culturing is still the most widely used method for determining fungal growth. Thus, is important to identify the most suitable culture media to assess Aspergillus spp. The aim of this study was to analyze data obtained from previous studies, aiming at identifying the most suitable culture media (malt extract agar (MEA) or dichloran-glycerol agar (DG18) to assess Aspergillus spp. isolation and growth. This study was conducted by using environmental samples (n = 1153). Most of the active sampling methods (air samples) were impacted directly onto both culture media. As for passive sampling methods, fungi were extracted from environmental matrices inoculated onto both media. Overall, total Aspergillus counts were higher in MEA (n = 617, 53.5%) than in DG18 (n = 536, 46.5%). Regarding Aspergillus sections, significant associations were detected with the media (χ2 (7) = 241.118, p < 0.001), the sampling approach (p < 0.001, 95% CI = (0.3 × 10−4 ), and the indoor environment (p < 0.001, 95% CI = (0.3 × 10−4 )). As such, sampling approach and the culture media should be accurately selected when dealing with Aspergillus spp. exposure assessment. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-02-01T23:31:58Z 2021-01 2021-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10362/111106 |
url |
http://hdl.handle.net/10362/111106 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
2073-4433 PURE: 27817615 https://doi.org/10.3390/atmos12010023 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
12 application/pdf |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799138030930362368 |