Cells released from S. epidermidis biofilms present increased antibiotic tolerance to multiple antibiotics
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/60420 |
Resumo: | Biofilm released cells (Brc) are thought to present an intermediary phenotype between biofilm and planktonic cells and this has the potential of affecting their antimicrobial tolerance. Aim Compare the antimicrobial tolerance profiles of Brc, planktonic or biofilm cultures of S. epidermidis. Methodology Planktonic, biofilm cultures or Brc from 11 isolates were exposed to peak serum concentrations (PSC) of antibiotics. The antimicrobial killing effect in the three populations was determined by CFU. Results Increased Brc tolerance to vancomycin, teicoplanin, rifampicin, erythromycin, and tetracycline was confirmed in model strain 9142. Furthermore, significant differences in the susceptibility of Brc to vancomycin were further found in 10 other clinical isolates. Conclusions Brc from distinct clinical isolates presented a decreased susceptibility to most antibiotics tested and maintained that enhanced tolerance despite growing planktonically for up to 6 h. Our data suggest that Brc maintain the typical enhanced antibiotic tolerance of biofilm populations, further suggesting that addressing antimicrobial susceptibility in planktonic cultures might not reflect the full potential of biofilm-associated bacteria to survive therapy. |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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7160 |
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Cells released from S. epidermidis biofilms present increased antibiotic tolerance to multiple antibioticsBiofilm disassemblyS. epidermidisBiofilm-released cellsAntimicrobial toleranceScience & TechnologyBiofilm released cells (Brc) are thought to present an intermediary phenotype between biofilm and planktonic cells and this has the potential of affecting their antimicrobial tolerance. Aim Compare the antimicrobial tolerance profiles of Brc, planktonic or biofilm cultures of S. epidermidis. Methodology Planktonic, biofilm cultures or Brc from 11 isolates were exposed to peak serum concentrations (PSC) of antibiotics. The antimicrobial killing effect in the three populations was determined by CFU. Results Increased Brc tolerance to vancomycin, teicoplanin, rifampicin, erythromycin, and tetracycline was confirmed in model strain 9142. Furthermore, significant differences in the susceptibility of Brc to vancomycin were further found in 10 other clinical isolates. Conclusions Brc from distinct clinical isolates presented a decreased susceptibility to most antibiotics tested and maintained that enhanced tolerance despite growing planktonically for up to 6 h. Our data suggest that Brc maintain the typical enhanced antibiotic tolerance of biofilm populations, further suggesting that addressing antimicrobial susceptibility in planktonic cultures might not reflect the full potential of biofilm-associated bacteria to survive therapy.This study was supported by the Portuguese Foundation for Science and Technology (FCT) under the scope of the strategic funding of UID/BIO/04469/2019 and by BioTecNorte operation(NORTE-01-0145-FEDER-000004)fundedbyEuropeanRegionalDevelopment Fund under the scope of Norte2020 - Programa Operacional Regional do Norte. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.info:eu-repo/semantics/publishedVersionPeerJ IncUniversidade do MinhoGaio, Vânia SilvaCerca, Nuno2019-052019-05-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/60420engGaio, Vania; Cerca, Nuno, Cells released from S. epidermidis biofilms present increased antibiotic tolerance to multiple antibiotics. PeerJ, 7(e6884), 20192167-835910.7717/peerj.6884https://peerj.com/info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:53:44Zoai:repositorium.sdum.uminho.pt:1822/60420Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:53:11.404163Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Cells released from S. epidermidis biofilms present increased antibiotic tolerance to multiple antibiotics |
title |
Cells released from S. epidermidis biofilms present increased antibiotic tolerance to multiple antibiotics |
spellingShingle |
Cells released from S. epidermidis biofilms present increased antibiotic tolerance to multiple antibiotics Gaio, Vânia Silva Biofilm disassembly S. epidermidis Biofilm-released cells Antimicrobial tolerance Science & Technology |
title_short |
Cells released from S. epidermidis biofilms present increased antibiotic tolerance to multiple antibiotics |
title_full |
Cells released from S. epidermidis biofilms present increased antibiotic tolerance to multiple antibiotics |
title_fullStr |
Cells released from S. epidermidis biofilms present increased antibiotic tolerance to multiple antibiotics |
title_full_unstemmed |
Cells released from S. epidermidis biofilms present increased antibiotic tolerance to multiple antibiotics |
title_sort |
Cells released from S. epidermidis biofilms present increased antibiotic tolerance to multiple antibiotics |
author |
Gaio, Vânia Silva |
author_facet |
Gaio, Vânia Silva Cerca, Nuno |
author_role |
author |
author2 |
Cerca, Nuno |
author2_role |
author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Gaio, Vânia Silva Cerca, Nuno |
dc.subject.por.fl_str_mv |
Biofilm disassembly S. epidermidis Biofilm-released cells Antimicrobial tolerance Science & Technology |
topic |
Biofilm disassembly S. epidermidis Biofilm-released cells Antimicrobial tolerance Science & Technology |
description |
Biofilm released cells (Brc) are thought to present an intermediary phenotype between biofilm and planktonic cells and this has the potential of affecting their antimicrobial tolerance. Aim Compare the antimicrobial tolerance profiles of Brc, planktonic or biofilm cultures of S. epidermidis. Methodology Planktonic, biofilm cultures or Brc from 11 isolates were exposed to peak serum concentrations (PSC) of antibiotics. The antimicrobial killing effect in the three populations was determined by CFU. Results Increased Brc tolerance to vancomycin, teicoplanin, rifampicin, erythromycin, and tetracycline was confirmed in model strain 9142. Furthermore, significant differences in the susceptibility of Brc to vancomycin were further found in 10 other clinical isolates. Conclusions Brc from distinct clinical isolates presented a decreased susceptibility to most antibiotics tested and maintained that enhanced tolerance despite growing planktonically for up to 6 h. Our data suggest that Brc maintain the typical enhanced antibiotic tolerance of biofilm populations, further suggesting that addressing antimicrobial susceptibility in planktonic cultures might not reflect the full potential of biofilm-associated bacteria to survive therapy. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-05 2019-05-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/60420 |
url |
http://hdl.handle.net/1822/60420 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Gaio, Vania; Cerca, Nuno, Cells released from S. epidermidis biofilms present increased antibiotic tolerance to multiple antibiotics. PeerJ, 7(e6884), 2019 2167-8359 10.7717/peerj.6884 https://peerj.com/ |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
PeerJ Inc |
publisher.none.fl_str_mv |
PeerJ Inc |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799133127002554368 |