Biofilm released cells can easily be obtained in a fed-batch system using ica+ but not with ica- isolates

Detalhes bibliográficos
Autor(a) principal: Gaio, Vânia Silva
Data de Publicação: 2020
Outros Autores: Cerca, Nuno
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/66966
Resumo: Supplemental information for this article can be found online at http://dx.doi.org/10.7717/peerj.9549#supplemental-information.
id RCAP_ea2b51e1f4781a46b172d1b3f4a54822
oai_identifier_str oai:repositorium.sdum.uminho.pt:1822/66966
network_acronym_str RCAP
network_name_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository_id_str 7160
spelling Biofilm released cells can easily be obtained in a fed-batch system using ica+ but not with ica- isolatesBiofilm-released cellsFed-batch systemsBiofilm disassemblyS. epidermidisScience & TechnologySupplemental information for this article can be found online at http://dx.doi.org/10.7717/peerj.9549#supplemental-information.Staphylococcus epidermidis is one of the major opportunistic bacterial pathogens in healthcare facilities, mainly due to its strong ability to form biofilms in the surface of indwelling medical devices. To study biofilms under in vitro conditions, both fed-batch and flow systems are widely used, with the first being the most frequent due to their low cost and ease of use. Aim To assess if a fed-batch system previously developed to obtain biofilm released cells (Brc) from strong biofilm producing S. epidermidis isolates could also be used to obtain and characterize Brc from isolates with lower abilities to form biofilms. Methodology The applicability of a fed-batch system to obtain Brc from biofilms of 3 ica+ and 3 ica isolates was assessed by quantifying the biofilm and Brc biomass by optical density (OD) and colony-forming units (CFU) measurements. The effect of media replacement procedures of fed-batch systems on the amount of biofilm was determined by quantifying the biofilm and biofilm bulk fluid, by CFU, after consecutive washing steps. Results The fed-batch model was appropriate to obtain Brc from ica+ isolates, that presented a greater ability to form biofilms and release cells. However, the same was not true for ica isolates, mainly because the washing procedure would physically remove a significant number of cells from the biofilm. Conclusions This study demonstrates that a fed-batch system is only feasible to be used to obtain Brc from S. epidermidis when studying strong and cohesive biofilm-forming isolates.This study was supported by the Portuguese Foundation for Science and Technology (FCT) under the scope of the strategic funding of UIDB/04469/2020 and by BioTecNorteoperation (NORTE-01-0145-FEDER-000004) funded by European Regional DevelopmentFund under the scope of Norte2020 - Programa Operacional Regional do Norte. Thefunders had no role in study design, data collection and analysis, decision to publish, orpreparation of the manuscript.info:eu-repo/semantics/publishedVersionPeerJ IncUniversidade do MinhoGaio, Vânia SilvaCerca, Nuno20202020-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/66966engGaio, Vania; Cerca, Nuno, Biofilm released cells can easily be obtained in a fed-batch system using ica+ but not with ica- isolates. PeerJ, 8(e9549), 2020. DOI: 10.7717/peerj.95492167835910.7717/peerj.9549https://peerj.com/articles/9549/info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:45:11Zoai:repositorium.sdum.uminho.pt:1822/66966Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:42:59.236163Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Biofilm released cells can easily be obtained in a fed-batch system using ica+ but not with ica- isolates
title Biofilm released cells can easily be obtained in a fed-batch system using ica+ but not with ica- isolates
spellingShingle Biofilm released cells can easily be obtained in a fed-batch system using ica+ but not with ica- isolates
Gaio, Vânia Silva
Biofilm-released cells
Fed-batch systems
Biofilm disassembly
S. epidermidis
Science & Technology
title_short Biofilm released cells can easily be obtained in a fed-batch system using ica+ but not with ica- isolates
title_full Biofilm released cells can easily be obtained in a fed-batch system using ica+ but not with ica- isolates
title_fullStr Biofilm released cells can easily be obtained in a fed-batch system using ica+ but not with ica- isolates
title_full_unstemmed Biofilm released cells can easily be obtained in a fed-batch system using ica+ but not with ica- isolates
title_sort Biofilm released cells can easily be obtained in a fed-batch system using ica+ but not with ica- isolates
author Gaio, Vânia Silva
author_facet Gaio, Vânia Silva
Cerca, Nuno
author_role author
author2 Cerca, Nuno
author2_role author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Gaio, Vânia Silva
Cerca, Nuno
dc.subject.por.fl_str_mv Biofilm-released cells
Fed-batch systems
Biofilm disassembly
S. epidermidis
Science & Technology
topic Biofilm-released cells
Fed-batch systems
Biofilm disassembly
S. epidermidis
Science & Technology
description Supplemental information for this article can be found online at http://dx.doi.org/10.7717/peerj.9549#supplemental-information.
publishDate 2020
dc.date.none.fl_str_mv 2020
2020-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/66966
url http://hdl.handle.net/1822/66966
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Gaio, Vania; Cerca, Nuno, Biofilm released cells can easily be obtained in a fed-batch system using ica+ but not with ica- isolates. PeerJ, 8(e9549), 2020. DOI: 10.7717/peerj.9549
21678359
10.7717/peerj.9549
https://peerj.com/articles/9549/
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv PeerJ Inc
publisher.none.fl_str_mv PeerJ Inc
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
_version_ 1799132985295896576

Registros relacionados