Characterization of an in vitro fed-batch model to obtain cells released from S. epidermidis biofilms
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/41195 |
Resumo: | Both dynamic and fed-batch systems have been used for the study of biofilms. Dynamic systems, whose hallmark is the presence of continuous flow, have been considered the most appropriate for the study of the last stage of the biofilm lifecycle: biofilm disassembly. However, fed-batch is still the most used system in the biofilm research field. Hence, we have used a fed-batch system to collect cells released from Staphylococcus epidermidis biofilms, one of the most important etiological agents of medical device-associated biofilm infections. Herein, we showed that using this model it was possible to collect cells released from biofilms formed by 12 different S. epidermidis clinical and commensal isolates. In addition, our data indicated that biofilm disassembly occurred by both passive and active mechanisms, although the last occurred to a lesser extent. Moreover, it was observed that S. epidermidis biofilm-released cells presented higher tolerance to vancomycin and tetracycline, as well as a particular gene expression phenotype when compared with either biofilm or planktonic cells. Using this model, biofilm-released cells phenotype and their interaction with the host immune system could be studied in more detail, which could help providing significant insights into the pathophysiology of biofilm-related infections. |
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Characterization of an in vitro fed-batch model to obtain cells released from S. epidermidis biofilmsFed-batch systemsBiofilmsBiofilm-released cellsS. epidermidisAntibiotic-toleranceGene expressionCiências Médicas::Biotecnologia MédicaScience & TechnologyBoth dynamic and fed-batch systems have been used for the study of biofilms. Dynamic systems, whose hallmark is the presence of continuous flow, have been considered the most appropriate for the study of the last stage of the biofilm lifecycle: biofilm disassembly. However, fed-batch is still the most used system in the biofilm research field. Hence, we have used a fed-batch system to collect cells released from Staphylococcus epidermidis biofilms, one of the most important etiological agents of medical device-associated biofilm infections. Herein, we showed that using this model it was possible to collect cells released from biofilms formed by 12 different S. epidermidis clinical and commensal isolates. In addition, our data indicated that biofilm disassembly occurred by both passive and active mechanisms, although the last occurred to a lesser extent. Moreover, it was observed that S. epidermidis biofilm-released cells presented higher tolerance to vancomycin and tetracycline, as well as a particular gene expression phenotype when compared with either biofilm or planktonic cells. Using this model, biofilm-released cells phenotype and their interaction with the host immune system could be studied in more detail, which could help providing significant insights into the pathophysiology of biofilm-related infections.European Union funds (FEDER/COMPETE) and by national funds (Fundação para a Ciência e a Tecnologia-FCT) under the project with reference FCOMP-01-0124-FEDER-041246 (EXPL/BIA-MIC/0101/2013). The authors thank the FCT Strategic Project of UID/BIO/04469/2013 unit and the project FCOMP-01-0124-FEDER-027462 (RECI/BBB-EBI/0179/2012); SFRH/BPD/99961/2014 and SFRH/BD/78235/2011SpringerUniversidade do MinhoFrança, ÂngelaCarvalhais, Virgínia Maria DinisVilanova, ManuelPier, Gerald B.Cerca, Nuno2016-032016-03-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/41195engFrança, Ângela; Carvalhais, V.; Pier, G. B.; Vilanova, M.; Cerca N, Characterization of an in vitro fed-batch model to obtain cells released from S. epidermidis biofilms. AMB Express, 6(23), 20162191-08552191085510.1186/s13568-016-0197-9http://amb-express.springeropen.com/articles/10.1186/s13568-016-0197-9info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:33:21Zoai:repositorium.sdum.uminho.pt:1822/41195Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:28:51.683244Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Characterization of an in vitro fed-batch model to obtain cells released from S. epidermidis biofilms |
title |
Characterization of an in vitro fed-batch model to obtain cells released from S. epidermidis biofilms |
spellingShingle |
Characterization of an in vitro fed-batch model to obtain cells released from S. epidermidis biofilms França, Ângela Fed-batch systems Biofilms Biofilm-released cells S. epidermidis Antibiotic-tolerance Gene expression Ciências Médicas::Biotecnologia Médica Science & Technology |
title_short |
Characterization of an in vitro fed-batch model to obtain cells released from S. epidermidis biofilms |
title_full |
Characterization of an in vitro fed-batch model to obtain cells released from S. epidermidis biofilms |
title_fullStr |
Characterization of an in vitro fed-batch model to obtain cells released from S. epidermidis biofilms |
title_full_unstemmed |
Characterization of an in vitro fed-batch model to obtain cells released from S. epidermidis biofilms |
title_sort |
Characterization of an in vitro fed-batch model to obtain cells released from S. epidermidis biofilms |
author |
França, Ângela |
author_facet |
França, Ângela Carvalhais, Virgínia Maria Dinis Vilanova, Manuel Pier, Gerald B. Cerca, Nuno |
author_role |
author |
author2 |
Carvalhais, Virgínia Maria Dinis Vilanova, Manuel Pier, Gerald B. Cerca, Nuno |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
França, Ângela Carvalhais, Virgínia Maria Dinis Vilanova, Manuel Pier, Gerald B. Cerca, Nuno |
dc.subject.por.fl_str_mv |
Fed-batch systems Biofilms Biofilm-released cells S. epidermidis Antibiotic-tolerance Gene expression Ciências Médicas::Biotecnologia Médica Science & Technology |
topic |
Fed-batch systems Biofilms Biofilm-released cells S. epidermidis Antibiotic-tolerance Gene expression Ciências Médicas::Biotecnologia Médica Science & Technology |
description |
Both dynamic and fed-batch systems have been used for the study of biofilms. Dynamic systems, whose hallmark is the presence of continuous flow, have been considered the most appropriate for the study of the last stage of the biofilm lifecycle: biofilm disassembly. However, fed-batch is still the most used system in the biofilm research field. Hence, we have used a fed-batch system to collect cells released from Staphylococcus epidermidis biofilms, one of the most important etiological agents of medical device-associated biofilm infections. Herein, we showed that using this model it was possible to collect cells released from biofilms formed by 12 different S. epidermidis clinical and commensal isolates. In addition, our data indicated that biofilm disassembly occurred by both passive and active mechanisms, although the last occurred to a lesser extent. Moreover, it was observed that S. epidermidis biofilm-released cells presented higher tolerance to vancomycin and tetracycline, as well as a particular gene expression phenotype when compared with either biofilm or planktonic cells. Using this model, biofilm-released cells phenotype and their interaction with the host immune system could be studied in more detail, which could help providing significant insights into the pathophysiology of biofilm-related infections. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-03 2016-03-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/41195 |
url |
http://hdl.handle.net/1822/41195 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
França, Ângela; Carvalhais, V.; Pier, G. B.; Vilanova, M.; Cerca N, Characterization of an in vitro fed-batch model to obtain cells released from S. epidermidis biofilms. AMB Express, 6(23), 2016 2191-0855 21910855 10.1186/s13568-016-0197-9 http://amb-express.springeropen.com/articles/10.1186/s13568-016-0197-9 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Springer |
publisher.none.fl_str_mv |
Springer |
dc.source.none.fl_str_mv |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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