Candida albicans adaptation on simulated human body fluids under different pH
Autor(a) principal: | |
---|---|
Data de Publicação: | 2020 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/65611 |
Resumo: | Candida albicans remains the most problematic of all Candida species, causing severe infections. Adaptation to different human body niches, such oral and urinary tracts, has been shown to be essential for survival and critical for virulence of C. albicans. Thus, the present work aimed to study the behaviour of C. albicans on simulated human body fluids (artificial saliva and urine) at different values of pH (pH 5.8 and 7) by determining its ability to develop two of the most important virulence factors: biofilms and filamentous forms. Under this study, it was demonstrated that C. albicans was able to grow as free cells and to develop biofilm communities composed of multiple cell types (yeast and elongated hyphal cells) on both simulated human body fluids and under different pH. It was interesting to note that the pH had little impact on C. albicans planktonic and biofilm growth, despite influencing the development of filamentous shapes in artificial saliva and urine. So, it was possible to infer that C. albicans presents a high plasticity and adaptability to different human body fluids, namely saliva and urine. These can be the justification for the high number of oral and urinary candidiasis in the whole world. |
id |
RCAP_ca9dfaf1be8ed00c84df546b1e284290 |
---|---|
oai_identifier_str |
oai:repositorium.sdum.uminho.pt:1822/65611 |
network_acronym_str |
RCAP |
network_name_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository_id_str |
7160 |
spelling |
Candida albicans adaptation on simulated human body fluids under different pHcandidiasispathogenicityenvironmental cuesvirulence factorScience & TechnologyCandida albicans remains the most problematic of all Candida species, causing severe infections. Adaptation to different human body niches, such oral and urinary tracts, has been shown to be essential for survival and critical for virulence of C. albicans. Thus, the present work aimed to study the behaviour of C. albicans on simulated human body fluids (artificial saliva and urine) at different values of pH (pH 5.8 and 7) by determining its ability to develop two of the most important virulence factors: biofilms and filamentous forms. Under this study, it was demonstrated that C. albicans was able to grow as free cells and to develop biofilm communities composed of multiple cell types (yeast and elongated hyphal cells) on both simulated human body fluids and under different pH. It was interesting to note that the pH had little impact on C. albicans planktonic and biofilm growth, despite influencing the development of filamentous shapes in artificial saliva and urine. So, it was possible to infer that C. albicans presents a high plasticity and adaptability to different human body fluids, namely saliva and urine. These can be the justification for the high number of oral and urinary candidiasis in the whole world.This study was supported by the Portuguese Foundation for Science and Technology (FCT) under the scope of the strategic funding of UIDB/04469/2020 unit and BioTecNorte operation (NORTE-01-0145-FEDER-000004) funded by the European Regional Development Fund under the scope of Norte2020—Programa Operacional Regional do Norte and Daniela Eira Araújo PhD grant (SFRH/BD/121417/2016). The authors also acknowledge the project funding by the “02/SAICT/2017—Projetos de Investigação Científica e Desenvolvimento Tecnológico (IC and DT)—POCI-01-0145-FEDER-028893”.info:eu-repo/semantics/publishedVersionMDPIUniversidade do MinhoBarbosa, AnaAraújo, DanielaRibeiro, EduardaHenriques, MarianaSilva, Sónia Carina2020-04-032020-04-03T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/65611engBarbosa, Ana; Araújo, Daniela; Ribeiro, Eduarda; Henriques, Mariana; Silva, Sónia Carina, Candida albicans adaptation on simulated human body fluids under different pH. Microorganisms, 8(4), 511, 20202076-26072076-260710.3390/microorganisms8040511https://www.mdpi.com/2076-2607/8/4/511info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:02:54Zoai:repositorium.sdum.uminho.pt:1822/65611Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:52:59.300691Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Candida albicans adaptation on simulated human body fluids under different pH |
title |
Candida albicans adaptation on simulated human body fluids under different pH |
spellingShingle |
Candida albicans adaptation on simulated human body fluids under different pH Barbosa, Ana candidiasis pathogenicity environmental cues virulence factor Science & Technology |
title_short |
Candida albicans adaptation on simulated human body fluids under different pH |
title_full |
Candida albicans adaptation on simulated human body fluids under different pH |
title_fullStr |
Candida albicans adaptation on simulated human body fluids under different pH |
title_full_unstemmed |
Candida albicans adaptation on simulated human body fluids under different pH |
title_sort |
Candida albicans adaptation on simulated human body fluids under different pH |
author |
Barbosa, Ana |
author_facet |
Barbosa, Ana Araújo, Daniela Ribeiro, Eduarda Henriques, Mariana Silva, Sónia Carina |
author_role |
author |
author2 |
Araújo, Daniela Ribeiro, Eduarda Henriques, Mariana Silva, Sónia Carina |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Barbosa, Ana Araújo, Daniela Ribeiro, Eduarda Henriques, Mariana Silva, Sónia Carina |
dc.subject.por.fl_str_mv |
candidiasis pathogenicity environmental cues virulence factor Science & Technology |
topic |
candidiasis pathogenicity environmental cues virulence factor Science & Technology |
description |
Candida albicans remains the most problematic of all Candida species, causing severe infections. Adaptation to different human body niches, such oral and urinary tracts, has been shown to be essential for survival and critical for virulence of C. albicans. Thus, the present work aimed to study the behaviour of C. albicans on simulated human body fluids (artificial saliva and urine) at different values of pH (pH 5.8 and 7) by determining its ability to develop two of the most important virulence factors: biofilms and filamentous forms. Under this study, it was demonstrated that C. albicans was able to grow as free cells and to develop biofilm communities composed of multiple cell types (yeast and elongated hyphal cells) on both simulated human body fluids and under different pH. It was interesting to note that the pH had little impact on C. albicans planktonic and biofilm growth, despite influencing the development of filamentous shapes in artificial saliva and urine. So, it was possible to infer that C. albicans presents a high plasticity and adaptability to different human body fluids, namely saliva and urine. These can be the justification for the high number of oral and urinary candidiasis in the whole world. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-04-03 2020-04-03T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/65611 |
url |
http://hdl.handle.net/1822/65611 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Barbosa, Ana; Araújo, Daniela; Ribeiro, Eduarda; Henriques, Mariana; Silva, Sónia Carina, Candida albicans adaptation on simulated human body fluids under different pH. Microorganisms, 8(4), 511, 2020 2076-2607 2076-2607 10.3390/microorganisms8040511 https://www.mdpi.com/2076-2607/8/4/511 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
MDPI |
publisher.none.fl_str_mv |
MDPI |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
|
_version_ |
1799132308129710080 |