One-pot low temperature synthesis and characterization of hybrid poly(2-pyrrolidone) microparticles suitable for protein immobilization

• Autor(a) principal: Dencheva, Nadya Vasileva
• Data de Publicação: 2018
• Outros Autores: Braz, Joana Filipa Barros, Nunes, Teresa G., Oliveira, Filipa D., Denchev, Z.
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
• Texto Completo: http://hdl.handle.net/1822/71582
• topic: Polyamide 4; 2-Pyrrolidone; Anionic ring-opening polymerization; Magnetic microparticles; Protein carriers; Science & Technology

Neat and hybrid poly(2-pyrrolidone), i.e. polyamide 4 (PA4) microparticles containing magnetic or conductive metal, metal oxide, or carbon nanotube fillers were prepared via environmentally-friendly solventless activated ring-opening polymerization of 2-pyrrolidone at 40 C. The method produces high porosity microparticles with diameters of 5e12mm and conversion to PA4 of 56e65%. Their structure and properties were characterized by light- and electron microscopy, thermal, spectral and X-ray diffraction techniques. Two crystalline polymorphs, namelya andb PА4, were found to coexist at room temperature by X-ray diffraction. The assessment of the adsorption capacity of the PA4 hybrid microparticles toward model protein showed up to 60% efficiency only after 1 h of incubation without any preliminary activation or functionalization.