Cues for cancer stem cells origin

Detalhes bibliográficos
Autor(a) principal: Gomes, Cátia Sofia Vicente
Data de Publicação: 2012
Tipo de documento: Dissertação
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/12439
Resumo: Dissertação para obtenção do Grau de Mestre em Genética Molecular e Biomedicina
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spelling Cues for cancer stem cells originAstrocytesNeural progenitor cellsNeural stem cellsGlioma cellsGliomagenesisTumour-related factorsDissertação para obtenção do Grau de Mestre em Genética Molecular e BiomedicinaNeural stem/progenitor cells (NSPC) can differentiate into neurons and glial cells in the central nervous system. Interestingly, NSPC biology is being applied to the study of human brain tumours, since these cells share some common features with glioma cells. However, it is not known the developmental stage with more similarities to glioma cells, or the most susceptible to malignant transformation. We aimed to identify the stage(s) in the NSPC differentiation process towards astrocytes where cells acquire phenotype characteristics comparable to glioma cells. NSPC that were obtained from E15 mouse brain, were grew as neurospheres (NS) and induced to astroglial differentiation until 7 days in vitro (DIV). After the cellular characterization of NS and differentiating cells, tumour-related factors were evaluated and their behavior compared to the one of GL261 mouse glioma cells. Astroglial differentiation led to a decrease in progenitor cells, as expected. Multidrug resistance-associated protein 1 expression decreased and autophagy marker increased with differentiation. The vascular endothelial growth factor (VEGF), matrix metalloproteinases and S100B protein increased until 2/3 DIV, while the 1 DIV cells showed the highest migratory potential towards the chemotactic VEGF or GL261-conditioned media. Comparison of data with glioma cells characteristics point to the first and second days of NSPC differentiation to astrocytes as the stages closing matching GL261 cells, and likely the most vulnerable to malignancy transformation.Faculdade de Ciências e TecnologiaBrites, DoraJesus, AnaRUNGomes, Cátia Sofia Vicente2014-07-09T15:45:07Z20122012-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfhttp://hdl.handle.net/10362/12439enginfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T03:47:20Zoai:run.unl.pt:10362/12439Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:20:53.829177Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Cues for cancer stem cells origin
title Cues for cancer stem cells origin
spellingShingle Cues for cancer stem cells origin
Gomes, Cátia Sofia Vicente
Astrocytes
Neural progenitor cells
Neural stem cells
Glioma cells
Gliomagenesis
Tumour-related factors
title_short Cues for cancer stem cells origin
title_full Cues for cancer stem cells origin
title_fullStr Cues for cancer stem cells origin
title_full_unstemmed Cues for cancer stem cells origin
title_sort Cues for cancer stem cells origin
author Gomes, Cátia Sofia Vicente
author_facet Gomes, Cátia Sofia Vicente
author_role author
dc.contributor.none.fl_str_mv Brites, Dora
Jesus, Ana
RUN
dc.contributor.author.fl_str_mv Gomes, Cátia Sofia Vicente
dc.subject.por.fl_str_mv Astrocytes
Neural progenitor cells
Neural stem cells
Glioma cells
Gliomagenesis
Tumour-related factors
topic Astrocytes
Neural progenitor cells
Neural stem cells
Glioma cells
Gliomagenesis
Tumour-related factors
description Dissertação para obtenção do Grau de Mestre em Genética Molecular e Biomedicina
publishDate 2012
dc.date.none.fl_str_mv 2012
2012-01-01T00:00:00Z
2014-07-09T15:45:07Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/12439
url http://hdl.handle.net/10362/12439
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Faculdade de Ciências e Tecnologia
publisher.none.fl_str_mv Faculdade de Ciências e Tecnologia
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
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reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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