A role for stargazin in experience-dependent plasticity

Detalhes bibliográficos
Autor(a) principal: Louros, Susana R.
Data de Publicação: 2014
Outros Autores: Hooks, Bryan M, Litvina, Liza, Carvalho, Ana Luísa, Chen, Chinfei
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10316/109384
https://doi.org/10.1016/j.celrep.2014.04.054
Resumo: During development, neurons are constantly refining their connections in response to changes in activity. Experience-dependent plasticity is a key form of synaptic plasticity, involving changes in α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor (AMPAR) accumulation at synapses. Here, we report a critical role for the AMPAR auxiliary subunit stargazin in this plasticity. We show that stargazin is functional at the retinogeniculate synapse and that in the absence of stargazin, the refinement of the retinogeniculate synapse is specifically disrupted during the experience-dependent phase. Importantly, we found that stargazin expression and phosphorylation increased with visual deprivation and led to reduced AMPAR rectification at the retinogeniculate synapse. To test whether stargazin plays a role in homeostatic plasticity, we turned to cultured neurons and found that stargazin phosphorylation is essential for synaptic scaling. Overall, our data reveal an important role for stargazin in regulating AMPAR abundance and composition at glutamatergic synapses during homeostatic and experience-dependent plasticity.
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spelling A role for stargazin in experience-dependent plasticityAnimalsCalcium ChannelsCells, CulturedGeniculate BodiesHomeostasisMiceNeuronsReceptors, AMPASynapsesLong-Term PotentiationDuring development, neurons are constantly refining their connections in response to changes in activity. Experience-dependent plasticity is a key form of synaptic plasticity, involving changes in α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor (AMPAR) accumulation at synapses. Here, we report a critical role for the AMPAR auxiliary subunit stargazin in this plasticity. We show that stargazin is functional at the retinogeniculate synapse and that in the absence of stargazin, the refinement of the retinogeniculate synapse is specifically disrupted during the experience-dependent phase. Importantly, we found that stargazin expression and phosphorylation increased with visual deprivation and led to reduced AMPAR rectification at the retinogeniculate synapse. To test whether stargazin plays a role in homeostatic plasticity, we turned to cultured neurons and found that stargazin phosphorylation is essential for synaptic scaling. Overall, our data reveal an important role for stargazin in regulating AMPAR abundance and composition at glutamatergic synapses during homeostatic and experience-dependent plasticity.2014-06-12info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://hdl.handle.net/10316/109384http://hdl.handle.net/10316/109384https://doi.org/10.1016/j.celrep.2014.04.054eng22111247Louros, Susana R.Hooks, Bryan MLitvina, LizaCarvalho, Ana LuísaChen, Chinfeiinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-10-12T08:28:44Zoai:estudogeral.uc.pt:10316/109384Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T21:25:34.979609Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv A role for stargazin in experience-dependent plasticity
title A role for stargazin in experience-dependent plasticity
spellingShingle A role for stargazin in experience-dependent plasticity
Louros, Susana R.
Animals
Calcium Channels
Cells, Cultured
Geniculate Bodies
Homeostasis
Mice
Neurons
Receptors, AMPA
Synapses
Long-Term Potentiation
title_short A role for stargazin in experience-dependent plasticity
title_full A role for stargazin in experience-dependent plasticity
title_fullStr A role for stargazin in experience-dependent plasticity
title_full_unstemmed A role for stargazin in experience-dependent plasticity
title_sort A role for stargazin in experience-dependent plasticity
author Louros, Susana R.
author_facet Louros, Susana R.
Hooks, Bryan M
Litvina, Liza
Carvalho, Ana Luísa
Chen, Chinfei
author_role author
author2 Hooks, Bryan M
Litvina, Liza
Carvalho, Ana Luísa
Chen, Chinfei
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Louros, Susana R.
Hooks, Bryan M
Litvina, Liza
Carvalho, Ana Luísa
Chen, Chinfei
dc.subject.por.fl_str_mv Animals
Calcium Channels
Cells, Cultured
Geniculate Bodies
Homeostasis
Mice
Neurons
Receptors, AMPA
Synapses
Long-Term Potentiation
topic Animals
Calcium Channels
Cells, Cultured
Geniculate Bodies
Homeostasis
Mice
Neurons
Receptors, AMPA
Synapses
Long-Term Potentiation
description During development, neurons are constantly refining their connections in response to changes in activity. Experience-dependent plasticity is a key form of synaptic plasticity, involving changes in α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor (AMPAR) accumulation at synapses. Here, we report a critical role for the AMPAR auxiliary subunit stargazin in this plasticity. We show that stargazin is functional at the retinogeniculate synapse and that in the absence of stargazin, the refinement of the retinogeniculate synapse is specifically disrupted during the experience-dependent phase. Importantly, we found that stargazin expression and phosphorylation increased with visual deprivation and led to reduced AMPAR rectification at the retinogeniculate synapse. To test whether stargazin plays a role in homeostatic plasticity, we turned to cultured neurons and found that stargazin phosphorylation is essential for synaptic scaling. Overall, our data reveal an important role for stargazin in regulating AMPAR abundance and composition at glutamatergic synapses during homeostatic and experience-dependent plasticity.
publishDate 2014
dc.date.none.fl_str_mv 2014-06-12
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10316/109384
http://hdl.handle.net/10316/109384
https://doi.org/10.1016/j.celrep.2014.04.054
url http://hdl.handle.net/10316/109384
https://doi.org/10.1016/j.celrep.2014.04.054
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 22111247
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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