A role for stargazin in experience-dependent plasticity
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10316/109384 https://doi.org/10.1016/j.celrep.2014.04.054 |
Resumo: | During development, neurons are constantly refining their connections in response to changes in activity. Experience-dependent plasticity is a key form of synaptic plasticity, involving changes in α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor (AMPAR) accumulation at synapses. Here, we report a critical role for the AMPAR auxiliary subunit stargazin in this plasticity. We show that stargazin is functional at the retinogeniculate synapse and that in the absence of stargazin, the refinement of the retinogeniculate synapse is specifically disrupted during the experience-dependent phase. Importantly, we found that stargazin expression and phosphorylation increased with visual deprivation and led to reduced AMPAR rectification at the retinogeniculate synapse. To test whether stargazin plays a role in homeostatic plasticity, we turned to cultured neurons and found that stargazin phosphorylation is essential for synaptic scaling. Overall, our data reveal an important role for stargazin in regulating AMPAR abundance and composition at glutamatergic synapses during homeostatic and experience-dependent plasticity. |
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A role for stargazin in experience-dependent plasticityAnimalsCalcium ChannelsCells, CulturedGeniculate BodiesHomeostasisMiceNeuronsReceptors, AMPASynapsesLong-Term PotentiationDuring development, neurons are constantly refining their connections in response to changes in activity. Experience-dependent plasticity is a key form of synaptic plasticity, involving changes in α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor (AMPAR) accumulation at synapses. Here, we report a critical role for the AMPAR auxiliary subunit stargazin in this plasticity. We show that stargazin is functional at the retinogeniculate synapse and that in the absence of stargazin, the refinement of the retinogeniculate synapse is specifically disrupted during the experience-dependent phase. Importantly, we found that stargazin expression and phosphorylation increased with visual deprivation and led to reduced AMPAR rectification at the retinogeniculate synapse. To test whether stargazin plays a role in homeostatic plasticity, we turned to cultured neurons and found that stargazin phosphorylation is essential for synaptic scaling. Overall, our data reveal an important role for stargazin in regulating AMPAR abundance and composition at glutamatergic synapses during homeostatic and experience-dependent plasticity.2014-06-12info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://hdl.handle.net/10316/109384http://hdl.handle.net/10316/109384https://doi.org/10.1016/j.celrep.2014.04.054eng22111247Louros, Susana R.Hooks, Bryan MLitvina, LizaCarvalho, Ana LuísaChen, Chinfeiinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-10-12T08:28:44Zoai:estudogeral.uc.pt:10316/109384Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T21:25:34.979609Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
A role for stargazin in experience-dependent plasticity |
title |
A role for stargazin in experience-dependent plasticity |
spellingShingle |
A role for stargazin in experience-dependent plasticity Louros, Susana R. Animals Calcium Channels Cells, Cultured Geniculate Bodies Homeostasis Mice Neurons Receptors, AMPA Synapses Long-Term Potentiation |
title_short |
A role for stargazin in experience-dependent plasticity |
title_full |
A role for stargazin in experience-dependent plasticity |
title_fullStr |
A role for stargazin in experience-dependent plasticity |
title_full_unstemmed |
A role for stargazin in experience-dependent plasticity |
title_sort |
A role for stargazin in experience-dependent plasticity |
author |
Louros, Susana R. |
author_facet |
Louros, Susana R. Hooks, Bryan M Litvina, Liza Carvalho, Ana Luísa Chen, Chinfei |
author_role |
author |
author2 |
Hooks, Bryan M Litvina, Liza Carvalho, Ana Luísa Chen, Chinfei |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Louros, Susana R. Hooks, Bryan M Litvina, Liza Carvalho, Ana Luísa Chen, Chinfei |
dc.subject.por.fl_str_mv |
Animals Calcium Channels Cells, Cultured Geniculate Bodies Homeostasis Mice Neurons Receptors, AMPA Synapses Long-Term Potentiation |
topic |
Animals Calcium Channels Cells, Cultured Geniculate Bodies Homeostasis Mice Neurons Receptors, AMPA Synapses Long-Term Potentiation |
description |
During development, neurons are constantly refining their connections in response to changes in activity. Experience-dependent plasticity is a key form of synaptic plasticity, involving changes in α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor (AMPAR) accumulation at synapses. Here, we report a critical role for the AMPAR auxiliary subunit stargazin in this plasticity. We show that stargazin is functional at the retinogeniculate synapse and that in the absence of stargazin, the refinement of the retinogeniculate synapse is specifically disrupted during the experience-dependent phase. Importantly, we found that stargazin expression and phosphorylation increased with visual deprivation and led to reduced AMPAR rectification at the retinogeniculate synapse. To test whether stargazin plays a role in homeostatic plasticity, we turned to cultured neurons and found that stargazin phosphorylation is essential for synaptic scaling. Overall, our data reveal an important role for stargazin in regulating AMPAR abundance and composition at glutamatergic synapses during homeostatic and experience-dependent plasticity. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-06-12 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10316/109384 http://hdl.handle.net/10316/109384 https://doi.org/10.1016/j.celrep.2014.04.054 |
url |
http://hdl.handle.net/10316/109384 https://doi.org/10.1016/j.celrep.2014.04.054 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
22111247 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1817553079316250624 |