Synthetic oligonucleotides: AFM characterisation and electroanalytical studies
Autor(a) principal: | |
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Data de Publicação: | 2005 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10316/5100 https://doi.org/10.1016/j.bioelechem.2004.06.008 |
Resumo: | One of the most important steps in designing more sensitive and stable DNA based biosensors is the immobilisation procedure of the nucleic acid probes on the transducer surface, while maintaining their conformational flexibility. MAC Mode AFM images in air demonstrated that the oligonucleotide sequences adsorb spontaneously on the electrode surface, showing the existence of pores in the adsorbed layer that reveal big parts of the electrode surface, which enables non-specific adsorption of other molecules on the uncovered areas. The electrostatic immobilisation onto a glassy carbon electrode followed by hybridisation with a complementary sequence and control with a non-complementary sequence was studied using differential pulse voltammetry and electrochemical impedance spectroscopy. Changes in the oxidation currents of guanosine and adenosine were observed after hybridisation events as well as after control experiments. Modification of the double layer capacitance that took place after hybridisation or control experiments showed that non-specific adsorption of complementary or non-complementary sequences occur allowing the formation of a mixed multilayer. |
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Synthetic oligonucleotides: AFM characterisation and electroanalytical studiesDNA biosensorHybridisationAdsorptionNon-specific adsorptionOne of the most important steps in designing more sensitive and stable DNA based biosensors is the immobilisation procedure of the nucleic acid probes on the transducer surface, while maintaining their conformational flexibility. MAC Mode AFM images in air demonstrated that the oligonucleotide sequences adsorb spontaneously on the electrode surface, showing the existence of pores in the adsorbed layer that reveal big parts of the electrode surface, which enables non-specific adsorption of other molecules on the uncovered areas. The electrostatic immobilisation onto a glassy carbon electrode followed by hybridisation with a complementary sequence and control with a non-complementary sequence was studied using differential pulse voltammetry and electrochemical impedance spectroscopy. Changes in the oxidation currents of guanosine and adenosine were observed after hybridisation events as well as after control experiments. Modification of the double layer capacitance that took place after hybridisation or control experiments showed that non-specific adsorption of complementary or non-complementary sequences occur allowing the formation of a mixed multilayer.http://www.sciencedirect.com/science/article/B6W72-4GP1VK1-1/1/c44e3d2a4c722d5cec59a4d09d0a744c2005info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleaplication/PDFhttp://hdl.handle.net/10316/5100http://hdl.handle.net/10316/5100https://doi.org/10.1016/j.bioelechem.2004.06.008engBioelectrochemistry. 67:2 (2005) 181-190Brett, A. M. OliveiraPaquim, A. M. ChiorceaDiculescu, V.Oretskaya, T. S.info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2020-11-06T16:48:50Zoai:estudogeral.uc.pt:10316/5100Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T21:01:38.758641Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Synthetic oligonucleotides: AFM characterisation and electroanalytical studies |
title |
Synthetic oligonucleotides: AFM characterisation and electroanalytical studies |
spellingShingle |
Synthetic oligonucleotides: AFM characterisation and electroanalytical studies Brett, A. M. Oliveira DNA biosensor Hybridisation Adsorption Non-specific adsorption |
title_short |
Synthetic oligonucleotides: AFM characterisation and electroanalytical studies |
title_full |
Synthetic oligonucleotides: AFM characterisation and electroanalytical studies |
title_fullStr |
Synthetic oligonucleotides: AFM characterisation and electroanalytical studies |
title_full_unstemmed |
Synthetic oligonucleotides: AFM characterisation and electroanalytical studies |
title_sort |
Synthetic oligonucleotides: AFM characterisation and electroanalytical studies |
author |
Brett, A. M. Oliveira |
author_facet |
Brett, A. M. Oliveira Paquim, A. M. Chiorcea Diculescu, V. Oretskaya, T. S. |
author_role |
author |
author2 |
Paquim, A. M. Chiorcea Diculescu, V. Oretskaya, T. S. |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Brett, A. M. Oliveira Paquim, A. M. Chiorcea Diculescu, V. Oretskaya, T. S. |
dc.subject.por.fl_str_mv |
DNA biosensor Hybridisation Adsorption Non-specific adsorption |
topic |
DNA biosensor Hybridisation Adsorption Non-specific adsorption |
description |
One of the most important steps in designing more sensitive and stable DNA based biosensors is the immobilisation procedure of the nucleic acid probes on the transducer surface, while maintaining their conformational flexibility. MAC Mode AFM images in air demonstrated that the oligonucleotide sequences adsorb spontaneously on the electrode surface, showing the existence of pores in the adsorbed layer that reveal big parts of the electrode surface, which enables non-specific adsorption of other molecules on the uncovered areas. The electrostatic immobilisation onto a glassy carbon electrode followed by hybridisation with a complementary sequence and control with a non-complementary sequence was studied using differential pulse voltammetry and electrochemical impedance spectroscopy. Changes in the oxidation currents of guanosine and adenosine were observed after hybridisation events as well as after control experiments. Modification of the double layer capacitance that took place after hybridisation or control experiments showed that non-specific adsorption of complementary or non-complementary sequences occur allowing the formation of a mixed multilayer. |
publishDate |
2005 |
dc.date.none.fl_str_mv |
2005 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10316/5100 http://hdl.handle.net/10316/5100 https://doi.org/10.1016/j.bioelechem.2004.06.008 |
url |
http://hdl.handle.net/10316/5100 https://doi.org/10.1016/j.bioelechem.2004.06.008 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Bioelectrochemistry. 67:2 (2005) 181-190 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
aplication/PDF |
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reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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